Strain Fitness in Escherichia coli ECRC62 around BNILDI_14710

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzzB and hisIE are separated by 95 nucleotideshisIE and hisF overlap by 7 nucleotideshisF and hisA overlap by 19 nucleotides BNILDI_14705: wzzB - LPS O-antigen chain length determinant protein WzzB, at 2,971,772 to 2,972,749 wzzB BNILDI_14710: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,972,845 to 2,973,456 hisIE BNILDI_14715: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 2,973,450 to 2,974,226 hisF BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945 hisA Position (kb) 2972 2973 2974Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2971.890 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.010 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.012 kb on + strand, within wzzBat 2972.013 kb on - strand, within wzzBat 2972.055 kb on + strand, within wzzBat 2972.055 kb on + strand, within wzzBat 2972.056 kb on - strand, within wzzBat 2972.177 kb on + strand, within wzzBat 2972.178 kb on - strand, within wzzBat 2972.178 kb on - strand, within wzzBat 2972.223 kb on - strand, within wzzBat 2972.259 kb on + strand, within wzzBat 2972.449 kb on + strand, within wzzBat 2972.449 kb on + strand, within wzzBat 2972.450 kb on - strand, within wzzBat 2972.452 kb on + strand, within wzzBat 2972.452 kb on + strand, within wzzBat 2972.453 kb on - strand, within wzzBat 2972.531 kb on + strand, within wzzBat 2972.606 kb on + strand, within wzzBat 2972.607 kb on - strand, within wzzBat 2972.629 kb on + strand, within wzzBat 2972.659 kb on + strandat 2972.659 kb on + strandat 2972.735 kb on + strandat 2972.735 kb on + strandat 2972.924 kb on + strand, within hisIEat 2972.924 kb on + strand, within hisIEat 2972.925 kb on - strand, within hisIEat 2972.925 kb on - strand, within hisIEat 2972.968 kb on + strand, within hisIEat 2972.968 kb on + strand, within hisIEat 2973.057 kb on - strand, within hisIEat 2973.228 kb on + strand, within hisIEat 2973.236 kb on - strand, within hisIEat 2973.348 kb on + strand, within hisIEat 2973.349 kb on - strand, within hisIEat 2973.351 kb on - strand, within hisIEat 2973.487 kb on + strandat 2973.496 kb on - strandat 2973.809 kb on - strand, within hisFat 2973.813 kb on - strand, within hisFat 2973.854 kb on + strand, within hisFat 2973.854 kb on + strand, within hisFat 2973.891 kb on + strand, within hisFat 2973.978 kb on - strand, within hisFat 2974.073 kb on + strand, within hisFat 2974.165 kb on - strandat 2974.165 kb on - strandat 2974.322 kb on - strand, within hisAat 2974.386 kb on + strand, within hisAat 2974.387 kb on - strand, within hisAat 2974.442 kb on + strand, within hisA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,971,890 + wzzB BNILDI_14705 0.12 +1.1
2,972,010 + wzzB BNILDI_14705 0.24 +0.3
2,972,010 + wzzB BNILDI_14705 0.24 +0.3
2,972,010 + wzzB BNILDI_14705 0.24 -3.8
2,972,010 + wzzB BNILDI_14705 0.24 +1.5
2,972,012 + wzzB BNILDI_14705 0.25 +0.3
2,972,012 + wzzB BNILDI_14705 0.25 +1.5
2,972,012 + wzzB BNILDI_14705 0.25 -0.7
2,972,013 - wzzB BNILDI_14705 0.25 -0.5
2,972,055 + wzzB BNILDI_14705 0.29 +1.7
2,972,055 + wzzB BNILDI_14705 0.29 +0.5
2,972,056 - wzzB BNILDI_14705 0.29 +0.2
2,972,177 + wzzB BNILDI_14705 0.41 +0.2
2,972,178 - wzzB BNILDI_14705 0.42 +0.3
2,972,178 - wzzB BNILDI_14705 0.42 +1.7
2,972,223 - wzzB BNILDI_14705 0.46 -0.5
2,972,259 + wzzB BNILDI_14705 0.50 +1.8
2,972,449 + wzzB BNILDI_14705 0.69 -0.3
2,972,449 + wzzB BNILDI_14705 0.69 -0.8
2,972,450 - wzzB BNILDI_14705 0.69 -0.4
2,972,452 + wzzB BNILDI_14705 0.70 +0.5
2,972,452 + wzzB BNILDI_14705 0.70 -0.4
2,972,453 - wzzB BNILDI_14705 0.70 +0.6
2,972,531 + wzzB BNILDI_14705 0.78 -1.1
2,972,606 + wzzB BNILDI_14705 0.85 +0.8
2,972,607 - wzzB BNILDI_14705 0.85 +0.0
2,972,629 + wzzB BNILDI_14705 0.88 +0.1
2,972,659 + -3.7
2,972,659 + -0.2
2,972,735 + +0.3
2,972,735 + -2.2
2,972,924 + hisIE BNILDI_14710 0.13 -1.8
2,972,924 + hisIE BNILDI_14710 0.13 +0.1
2,972,925 - hisIE BNILDI_14710 0.13 -2.3
2,972,925 - hisIE BNILDI_14710 0.13 -1.9
2,972,968 + hisIE BNILDI_14710 0.20 -2.8
2,972,968 + hisIE BNILDI_14710 0.20 -1.4
2,973,057 - hisIE BNILDI_14710 0.35 -2.0
2,973,228 + hisIE BNILDI_14710 0.63 -3.1
2,973,236 - hisIE BNILDI_14710 0.64 -3.4
2,973,348 + hisIE BNILDI_14710 0.82 -3.9
2,973,349 - hisIE BNILDI_14710 0.82 -5.6
2,973,351 - hisIE BNILDI_14710 0.83 -4.6
2,973,487 + -3.8
2,973,496 - -2.8
2,973,809 - hisF BNILDI_14715 0.46 -4.9
2,973,813 - hisF BNILDI_14715 0.47 -2.9
2,973,854 + hisF BNILDI_14715 0.52 -3.6
2,973,854 + hisF BNILDI_14715 0.52 -2.5
2,973,891 + hisF BNILDI_14715 0.57 -1.9
2,973,978 - hisF BNILDI_14715 0.68 -4.5
2,974,073 + hisF BNILDI_14715 0.80 +0.2
2,974,165 - -4.6
2,974,165 - -5.0
2,974,322 - hisA BNILDI_14720 0.15 -2.8
2,974,386 + hisA BNILDI_14720 0.24 -3.1
2,974,387 - hisA BNILDI_14720 0.24 -2.5
2,974,442 + hisA BNILDI_14720 0.32 -3.2

Or see this region's nucleotide sequence