Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzzB and hisIE are separated by 95 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides
BNILDI_14705: wzzB - LPS O-antigen chain length determinant protein WzzB, at 2,971,772 to 2,972,749
wzzB
BNILDI_14710: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,972,845 to 2,973,456
hisIE
BNILDI_14715: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 2,973,450 to 2,974,226
hisF
BNILDI_14720: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 2,974,208 to 2,974,945
hisA
Position (kb)
2972
2973
2974 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2971.890 kb on + strand, within wzzB at 2972.010 kb on + strand, within wzzB at 2972.010 kb on + strand, within wzzB at 2972.010 kb on + strand, within wzzB at 2972.010 kb on + strand, within wzzB at 2972.012 kb on + strand, within wzzB at 2972.012 kb on + strand, within wzzB at 2972.012 kb on + strand, within wzzB at 2972.013 kb on - strand, within wzzB at 2972.055 kb on + strand, within wzzB at 2972.055 kb on + strand, within wzzB at 2972.056 kb on - strand, within wzzB at 2972.177 kb on + strand, within wzzB at 2972.178 kb on - strand, within wzzB at 2972.178 kb on - strand, within wzzB at 2972.223 kb on - strand, within wzzB at 2972.259 kb on + strand, within wzzB at 2972.449 kb on + strand, within wzzB at 2972.449 kb on + strand, within wzzB at 2972.450 kb on - strand, within wzzB at 2972.452 kb on + strand, within wzzB at 2972.452 kb on + strand, within wzzB at 2972.453 kb on - strand, within wzzB at 2972.531 kb on + strand, within wzzB at 2972.606 kb on + strand, within wzzB at 2972.607 kb on - strand, within wzzB at 2972.629 kb on + strand, within wzzB at 2972.659 kb on + strand at 2972.659 kb on + strand at 2972.735 kb on + strand at 2972.735 kb on + strand at 2972.924 kb on + strand, within hisIE at 2972.924 kb on + strand, within hisIE at 2972.925 kb on - strand, within hisIE at 2972.925 kb on - strand, within hisIE at 2972.968 kb on + strand, within hisIE at 2972.968 kb on + strand, within hisIE at 2973.057 kb on - strand, within hisIE at 2973.228 kb on + strand, within hisIE at 2973.236 kb on - strand, within hisIE at 2973.348 kb on + strand, within hisIE at 2973.349 kb on - strand, within hisIE at 2973.351 kb on - strand, within hisIE at 2973.487 kb on + strand at 2973.496 kb on - strand at 2973.809 kb on - strand, within hisF at 2973.813 kb on - strand, within hisF at 2973.854 kb on + strand, within hisF at 2973.854 kb on + strand, within hisF at 2973.891 kb on + strand, within hisF at 2973.978 kb on - strand, within hisF at 2974.073 kb on + strand, within hisF at 2974.165 kb on - strand at 2974.165 kb on - strand at 2974.322 kb on - strand, within hisA at 2974.386 kb on + strand, within hisA at 2974.387 kb on - strand, within hisA at 2974.442 kb on + strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,971,890 + wzzB BNILDI_14705 0.12 +1.1 2,972,010 + wzzB BNILDI_14705 0.24 +0.3 2,972,010 + wzzB BNILDI_14705 0.24 +0.3 2,972,010 + wzzB BNILDI_14705 0.24 -3.8 2,972,010 + wzzB BNILDI_14705 0.24 +1.5 2,972,012 + wzzB BNILDI_14705 0.25 +0.3 2,972,012 + wzzB BNILDI_14705 0.25 +1.5 2,972,012 + wzzB BNILDI_14705 0.25 -0.7 2,972,013 - wzzB BNILDI_14705 0.25 -0.5 2,972,055 + wzzB BNILDI_14705 0.29 +1.7 2,972,055 + wzzB BNILDI_14705 0.29 +0.5 2,972,056 - wzzB BNILDI_14705 0.29 +0.2 2,972,177 + wzzB BNILDI_14705 0.41 +0.2 2,972,178 - wzzB BNILDI_14705 0.42 +0.3 2,972,178 - wzzB BNILDI_14705 0.42 +1.7 2,972,223 - wzzB BNILDI_14705 0.46 -0.5 2,972,259 + wzzB BNILDI_14705 0.50 +1.8 2,972,449 + wzzB BNILDI_14705 0.69 -0.3 2,972,449 + wzzB BNILDI_14705 0.69 -0.8 2,972,450 - wzzB BNILDI_14705 0.69 -0.4 2,972,452 + wzzB BNILDI_14705 0.70 +0.5 2,972,452 + wzzB BNILDI_14705 0.70 -0.4 2,972,453 - wzzB BNILDI_14705 0.70 +0.6 2,972,531 + wzzB BNILDI_14705 0.78 -1.1 2,972,606 + wzzB BNILDI_14705 0.85 +0.8 2,972,607 - wzzB BNILDI_14705 0.85 +0.0 2,972,629 + wzzB BNILDI_14705 0.88 +0.1 2,972,659 + -3.7 2,972,659 + -0.2 2,972,735 + +0.3 2,972,735 + -2.2 2,972,924 + hisIE BNILDI_14710 0.13 -1.8 2,972,924 + hisIE BNILDI_14710 0.13 +0.1 2,972,925 - hisIE BNILDI_14710 0.13 -2.3 2,972,925 - hisIE BNILDI_14710 0.13 -1.9 2,972,968 + hisIE BNILDI_14710 0.20 -2.8 2,972,968 + hisIE BNILDI_14710 0.20 -1.4 2,973,057 - hisIE BNILDI_14710 0.35 -2.0 2,973,228 + hisIE BNILDI_14710 0.63 -3.1 2,973,236 - hisIE BNILDI_14710 0.64 -3.4 2,973,348 + hisIE BNILDI_14710 0.82 -3.9 2,973,349 - hisIE BNILDI_14710 0.82 -5.6 2,973,351 - hisIE BNILDI_14710 0.83 -4.6 2,973,487 + -3.8 2,973,496 - -2.8 2,973,809 - hisF BNILDI_14715 0.46 -4.9 2,973,813 - hisF BNILDI_14715 0.47 -2.9 2,973,854 + hisF BNILDI_14715 0.52 -3.6 2,973,854 + hisF BNILDI_14715 0.52 -2.5 2,973,891 + hisF BNILDI_14715 0.57 -1.9 2,973,978 - hisF BNILDI_14715 0.68 -4.5 2,974,073 + hisF BNILDI_14715 0.80 +0.2 2,974,165 - -4.6 2,974,165 - -5.0 2,974,322 - hisA BNILDI_14720 0.15 -2.8 2,974,386 + hisA BNILDI_14720 0.24 -3.1 2,974,387 - hisA BNILDI_14720 0.24 -2.5 2,974,442 + hisA BNILDI_14720 0.32 -3.2
Or see this region's nucleotide sequence