Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt arnB and ais are separated by 289 nucleotides ais and nudI are separated by 38 nucleotides nudI and yfaZ are separated by 278 nucleotides
BNILDI_13535: arnB - UDP-4-amino-4-deoxy-L-arabinose aminotransferase, at 2,713,574 to 2,714,731
arnB
BNILDI_13540: ais - Lipopolysaccharide core heptose(II)-phosphate phosphatase, at 2,715,021 to 2,715,623
ais
BNILDI_13545: nudI - nucleoside triphosphatase NudI, at 2,715,662 to 2,716,087
nudI
BNILDI_13550: yfaZ - Outer membrane protein YfaZ, at 2,716,366 to 2,716,908
yfaZ
Position (kb)
2715
2716 Strain fitness (log2 ratio)
-2
-1
0
1 at 2714.068 kb on - strand, within arnB at 2714.135 kb on + strand, within arnB at 2714.165 kb on - strand, within arnB at 2714.192 kb on - strand, within arnB at 2714.212 kb on + strand, within arnB at 2714.212 kb on + strand, within arnB at 2714.227 kb on - strand, within arnB at 2714.229 kb on + strand, within arnB at 2714.229 kb on + strand, within arnB at 2714.325 kb on + strand, within arnB at 2714.325 kb on + strand, within arnB at 2714.325 kb on + strand, within arnB at 2714.325 kb on + strand, within arnB at 2714.450 kb on - strand, within arnB at 2714.482 kb on + strand, within arnB at 2714.527 kb on + strand, within arnB at 2714.528 kb on - strand, within arnB at 2714.750 kb on + strand at 2715.073 kb on + strand at 2715.141 kb on - strand, within ais at 2715.149 kb on + strand, within ais at 2715.255 kb on - strand, within ais at 2715.257 kb on + strand, within ais at 2715.263 kb on + strand, within ais at 2715.339 kb on - strand, within ais at 2715.342 kb on - strand, within ais at 2715.397 kb on + strand, within ais at 2715.510 kb on + strand, within ais at 2715.510 kb on + strand, within ais at 2715.513 kb on - strand, within ais at 2715.811 kb on - strand, within nudI at 2715.846 kb on + strand, within nudI at 2716.192 kb on - strand at 2716.459 kb on + strand, within yfaZ at 2716.460 kb on - strand, within yfaZ at 2716.460 kb on - strand, within yfaZ at 2716.527 kb on + strand, within yfaZ at 2716.528 kb on - strand, within yfaZ at 2716.618 kb on + strand, within yfaZ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,714,068 - arnB BNILDI_13535 0.43 +0.6 2,714,135 + arnB BNILDI_13535 0.48 +0.0 2,714,165 - arnB BNILDI_13535 0.51 +0.2 2,714,192 - arnB BNILDI_13535 0.53 -0.2 2,714,212 + arnB BNILDI_13535 0.55 +0.3 2,714,212 + arnB BNILDI_13535 0.55 +0.1 2,714,227 - arnB BNILDI_13535 0.56 +0.9 2,714,229 + arnB BNILDI_13535 0.57 -0.5 2,714,229 + arnB BNILDI_13535 0.57 +0.1 2,714,325 + arnB BNILDI_13535 0.65 +0.5 2,714,325 + arnB BNILDI_13535 0.65 +0.1 2,714,325 + arnB BNILDI_13535 0.65 -0.1 2,714,325 + arnB BNILDI_13535 0.65 -0.5 2,714,450 - arnB BNILDI_13535 0.76 +1.5 2,714,482 + arnB BNILDI_13535 0.78 -0.4 2,714,527 + arnB BNILDI_13535 0.82 +0.1 2,714,528 - arnB BNILDI_13535 0.82 -0.9 2,714,750 + -2.4 2,715,073 + -0.3 2,715,141 - ais BNILDI_13540 0.20 +0.2 2,715,149 + ais BNILDI_13540 0.21 +1.6 2,715,255 - ais BNILDI_13540 0.39 +0.8 2,715,257 + ais BNILDI_13540 0.39 -0.4 2,715,263 + ais BNILDI_13540 0.40 +0.4 2,715,339 - ais BNILDI_13540 0.53 -1.0 2,715,342 - ais BNILDI_13540 0.53 -0.7 2,715,397 + ais BNILDI_13540 0.62 -0.2 2,715,510 + ais BNILDI_13540 0.81 -0.4 2,715,510 + ais BNILDI_13540 0.81 +0.3 2,715,513 - ais BNILDI_13540 0.82 +0.2 2,715,811 - nudI BNILDI_13545 0.35 -0.2 2,715,846 + nudI BNILDI_13545 0.43 -0.3 2,716,192 - -0.4 2,716,459 + yfaZ BNILDI_13550 0.17 -0.0 2,716,460 - yfaZ BNILDI_13550 0.17 -0.7 2,716,460 - yfaZ BNILDI_13550 0.17 -0.8 2,716,527 + yfaZ BNILDI_13550 0.30 -0.2 2,716,528 - yfaZ BNILDI_13550 0.30 -1.5 2,716,618 + yfaZ BNILDI_13550 0.46 -1.1
Or see this region's nucleotide sequence