Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppx and ppk1 are separated by 4 nucleotides ppk1 and purN are separated by 170 nucleotides purN and purM overlap by 1 nucleotides
BNILDI_12255: ppx - exopolyphosphatase, at 2,459,690 to 2,461,231
ppx
BNILDI_12260: ppk1 - polyphosphate kinase 1, at 2,461,236 to 2,463,302
ppk1
BNILDI_12265: purN - phosphoribosylglycinamide formyltransferase, at 2,463,473 to 2,464,111
purN
BNILDI_12270: purM - Phosphoribosylformylglycinamidine cyclo-ligase, at 2,464,111 to 2,465,148
purM
Position (kb)
2461
2462
2463
2464 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2460.325 kb on - strand, within ppx at 2460.342 kb on - strand, within ppx at 2460.377 kb on + strand, within ppx at 2460.706 kb on - strand, within ppx at 2460.706 kb on - strand, within ppx at 2460.755 kb on - strand, within ppx at 2460.838 kb on + strand, within ppx at 2460.838 kb on + strand, within ppx at 2460.974 kb on + strand, within ppx at 2460.975 kb on - strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.066 kb on + strand, within ppx at 2461.069 kb on + strand, within ppx at 2461.069 kb on + strand, within ppx at 2461.140 kb on - strand at 2461.152 kb on - strand at 2461.152 kb on - strand at 2461.216 kb on + strand at 2461.265 kb on - strand at 2461.270 kb on + strand at 2461.270 kb on + strand at 2461.271 kb on - strand at 2461.271 kb on - strand at 2461.374 kb on + strand at 2461.375 kb on - strand at 2461.375 kb on - strand at 2461.375 kb on - strand at 2461.538 kb on - strand, within ppk1 at 2461.550 kb on + strand, within ppk1 at 2461.655 kb on - strand, within ppk1 at 2461.688 kb on + strand, within ppk1 at 2461.689 kb on - strand, within ppk1 at 2461.693 kb on - strand, within ppk1 at 2461.693 kb on - strand, within ppk1 at 2461.749 kb on + strand, within ppk1 at 2461.750 kb on - strand, within ppk1 at 2461.760 kb on - strand, within ppk1 at 2461.794 kb on + strand, within ppk1 at 2461.795 kb on - strand, within ppk1 at 2461.838 kb on + strand, within ppk1 at 2462.027 kb on + strand, within ppk1 at 2462.028 kb on - strand, within ppk1 at 2462.073 kb on - strand, within ppk1 at 2462.124 kb on - strand, within ppk1 at 2462.130 kb on - strand, within ppk1 at 2462.264 kb on - strand, within ppk1 at 2462.272 kb on + strand, within ppk1 at 2462.340 kb on - strand, within ppk1 at 2462.340 kb on - strand, within ppk1 at 2462.403 kb on - strand, within ppk1 at 2462.459 kb on + strand, within ppk1 at 2462.538 kb on + strand, within ppk1 at 2462.599 kb on - strand, within ppk1 at 2462.635 kb on + strand, within ppk1 at 2462.635 kb on + strand, within ppk1 at 2462.842 kb on + strand, within ppk1 at 2462.843 kb on - strand, within ppk1 at 2462.843 kb on - strand, within ppk1 at 2462.914 kb on + strand, within ppk1 at 2463.010 kb on - strand, within ppk1 at 2463.019 kb on + strand, within ppk1 at 2463.019 kb on + strand, within ppk1 at 2463.154 kb on + strand at 2463.155 kb on - strand at 2463.178 kb on + strand at 2463.283 kb on + strand at 2463.283 kb on + strand at 2463.284 kb on - strand at 2463.286 kb on - strand at 2463.313 kb on - strand at 2463.344 kb on + strand at 2463.344 kb on + strand at 2463.347 kb on + strand at 2463.377 kb on + strand at 2463.464 kb on + strand at 2463.568 kb on + strand, within purN at 2463.712 kb on + strand, within purN at 2463.768 kb on + strand, within purN at 2463.879 kb on + strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.880 kb on - strand, within purN at 2463.913 kb on - strand, within purN at 2464.042 kb on + strand, within purN at 2464.061 kb on - strand at 2464.093 kb on + strand at 2464.093 kb on + strand at 2464.094 kb on - strand at 2464.106 kb on - strand at 2464.266 kb on - strand, within purM at 2464.288 kb on - strand, within purM
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,460,325 - ppx BNILDI_12255 0.41 +1.0 2,460,342 - ppx BNILDI_12255 0.42 -0.4 2,460,377 + ppx BNILDI_12255 0.45 -0.5 2,460,706 - ppx BNILDI_12255 0.66 -1.0 2,460,706 - ppx BNILDI_12255 0.66 -0.1 2,460,755 - ppx BNILDI_12255 0.69 -0.9 2,460,838 + ppx BNILDI_12255 0.74 +0.1 2,460,838 + ppx BNILDI_12255 0.74 -0.4 2,460,974 + ppx BNILDI_12255 0.83 -0.1 2,460,975 - ppx BNILDI_12255 0.83 +1.1 2,461,066 + ppx BNILDI_12255 0.89 -1.4 2,461,066 + ppx BNILDI_12255 0.89 +2.0 2,461,066 + ppx BNILDI_12255 0.89 -0.4 2,461,069 + ppx BNILDI_12255 0.89 +1.1 2,461,069 + ppx BNILDI_12255 0.89 -0.8 2,461,140 - -2.2 2,461,152 - -1.7 2,461,152 - -0.4 2,461,216 + -0.8 2,461,265 - -0.2 2,461,270 + -0.4 2,461,270 + -0.6 2,461,271 - -0.8 2,461,271 - -0.9 2,461,374 + +0.8 2,461,375 - -0.5 2,461,375 - +0.0 2,461,375 - -0.4 2,461,538 - ppk1 BNILDI_12260 0.15 -0.6 2,461,550 + ppk1 BNILDI_12260 0.15 -0.5 2,461,655 - ppk1 BNILDI_12260 0.20 -0.6 2,461,688 + ppk1 BNILDI_12260 0.22 -0.6 2,461,689 - ppk1 BNILDI_12260 0.22 -0.2 2,461,693 - ppk1 BNILDI_12260 0.22 -0.1 2,461,693 - ppk1 BNILDI_12260 0.22 -1.1 2,461,749 + ppk1 BNILDI_12260 0.25 -0.8 2,461,750 - ppk1 BNILDI_12260 0.25 -0.8 2,461,760 - ppk1 BNILDI_12260 0.25 -0.1 2,461,794 + ppk1 BNILDI_12260 0.27 -0.5 2,461,795 - ppk1 BNILDI_12260 0.27 -1.3 2,461,838 + ppk1 BNILDI_12260 0.29 -3.4 2,462,027 + ppk1 BNILDI_12260 0.38 -1.8 2,462,028 - ppk1 BNILDI_12260 0.38 +0.5 2,462,073 - ppk1 BNILDI_12260 0.40 -1.7 2,462,124 - ppk1 BNILDI_12260 0.43 -0.8 2,462,130 - ppk1 BNILDI_12260 0.43 -0.5 2,462,264 - ppk1 BNILDI_12260 0.50 -0.2 2,462,272 + ppk1 BNILDI_12260 0.50 +0.7 2,462,340 - ppk1 BNILDI_12260 0.53 +0.9 2,462,340 - ppk1 BNILDI_12260 0.53 -0.6 2,462,403 - ppk1 BNILDI_12260 0.56 -0.3 2,462,459 + ppk1 BNILDI_12260 0.59 -0.1 2,462,538 + ppk1 BNILDI_12260 0.63 -0.7 2,462,599 - ppk1 BNILDI_12260 0.66 -0.1 2,462,635 + ppk1 BNILDI_12260 0.68 +0.5 2,462,635 + ppk1 BNILDI_12260 0.68 -0.5 2,462,842 + ppk1 BNILDI_12260 0.78 -0.5 2,462,843 - ppk1 BNILDI_12260 0.78 -0.6 2,462,843 - ppk1 BNILDI_12260 0.78 +0.4 2,462,914 + ppk1 BNILDI_12260 0.81 -1.1 2,463,010 - ppk1 BNILDI_12260 0.86 +0.2 2,463,019 + ppk1 BNILDI_12260 0.86 -0.8 2,463,019 + ppk1 BNILDI_12260 0.86 +2.1 2,463,154 + -1.1 2,463,155 - -2.4 2,463,178 + +0.3 2,463,283 + +1.6 2,463,283 + -0.3 2,463,284 - -0.3 2,463,286 - +0.8 2,463,313 - -0.2 2,463,344 + -0.5 2,463,344 + -0.4 2,463,347 + +0.1 2,463,377 + +0.7 2,463,464 + +0.4 2,463,568 + purN BNILDI_12265 0.15 -3.5 2,463,712 + purN BNILDI_12265 0.37 -0.0 2,463,768 + purN BNILDI_12265 0.46 -1.4 2,463,879 + purN BNILDI_12265 0.64 -2.6 2,463,880 - purN BNILDI_12265 0.64 -0.3 2,463,880 - purN BNILDI_12265 0.64 -2.3 2,463,880 - purN BNILDI_12265 0.64 -1.9 2,463,880 - purN BNILDI_12265 0.64 -2.5 2,463,880 - purN BNILDI_12265 0.64 -1.0 2,463,913 - purN BNILDI_12265 0.69 -0.4 2,464,042 + purN BNILDI_12265 0.89 -0.1 2,464,061 - -0.9 2,464,093 + +0.2 2,464,093 + -1.5 2,464,094 - +1.1 2,464,106 - -0.1 2,464,266 - purM BNILDI_12270 0.15 -1.7 2,464,288 - purM BNILDI_12270 0.17 -3.5
Or see this region's nucleotide sequence