Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaA and yfgI are separated by 94 nucleotides yfgI and yfgH are separated by 15 nucleotides yfgH and tnp-IS3 are separated by 380 nucleotides
BNILDI_12215: guaA - glutamine-hydrolyzing GMP synthase, at 2,452,548 to 2,454,125
guaA
BNILDI_12220: yfgI - Uncharacterized protein YfgI, at 2,454,220 to 2,454,759
yfgI
BNILDI_12225: yfgH - Uncharacterized lipoprotein YfgH, at 2,454,775 to 2,455,290
yfgH
BNILDI_12230: tnp-IS3 - IS3 family transposase, at 2,455,671 to 2,456,537
tnp-IS3
Position (kb)
2454
2455 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2453.320 kb on + strand, within guaA at 2453.372 kb on + strand, within guaA at 2453.373 kb on - strand, within guaA at 2453.500 kb on + strand, within guaA at 2453.742 kb on + strand, within guaA at 2453.855 kb on + strand, within guaA at 2453.908 kb on + strand, within guaA at 2453.939 kb on + strand, within guaA at 2453.940 kb on - strand, within guaA at 2454.445 kb on + strand, within yfgI at 2454.446 kb on - strand, within yfgI at 2454.722 kb on - strand at 2454.722 kb on - strand at 2454.774 kb on - strand at 2454.777 kb on - strand at 2454.824 kb on + strand at 2454.824 kb on + strand at 2454.825 kb on - strand at 2454.838 kb on - strand, within yfgH at 2454.893 kb on + strand, within yfgH at 2454.894 kb on - strand, within yfgH at 2454.894 kb on - strand, within yfgH at 2454.912 kb on + strand, within yfgH at 2454.912 kb on + strand, within yfgH at 2454.912 kb on + strand, within yfgH at 2454.914 kb on + strand, within yfgH at 2454.938 kb on - strand, within yfgH at 2454.957 kb on + strand, within yfgH at 2454.958 kb on - strand, within yfgH at 2455.143 kb on + strand, within yfgH at 2455.648 kb on - strand at 2455.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,453,320 + guaA BNILDI_12215 0.49 -3.2 2,453,372 + guaA BNILDI_12215 0.52 -2.0 2,453,373 - guaA BNILDI_12215 0.52 -2.2 2,453,500 + guaA BNILDI_12215 0.60 -2.1 2,453,742 + guaA BNILDI_12215 0.76 -5.2 2,453,855 + guaA BNILDI_12215 0.83 -1.1 2,453,908 + guaA BNILDI_12215 0.86 -1.8 2,453,939 + guaA BNILDI_12215 0.88 -2.5 2,453,940 - guaA BNILDI_12215 0.88 -3.1 2,454,445 + yfgI BNILDI_12220 0.42 -2.1 2,454,446 - yfgI BNILDI_12220 0.42 -1.7 2,454,722 - +0.0 2,454,722 - -0.2 2,454,774 - -0.0 2,454,777 - +1.0 2,454,824 + +0.3 2,454,824 + -0.1 2,454,825 - -0.1 2,454,838 - yfgH BNILDI_12225 0.12 +0.7 2,454,893 + yfgH BNILDI_12225 0.23 +0.8 2,454,894 - yfgH BNILDI_12225 0.23 -0.2 2,454,894 - yfgH BNILDI_12225 0.23 -1.3 2,454,912 + yfgH BNILDI_12225 0.27 -0.9 2,454,912 + yfgH BNILDI_12225 0.27 -0.7 2,454,912 + yfgH BNILDI_12225 0.27 +1.2 2,454,914 + yfgH BNILDI_12225 0.27 -0.1 2,454,938 - yfgH BNILDI_12225 0.32 +2.2 2,454,957 + yfgH BNILDI_12225 0.35 +0.6 2,454,958 - yfgH BNILDI_12225 0.35 +0.5 2,455,143 + yfgH BNILDI_12225 0.71 +0.2 2,455,648 - -0.1 2,455,656 - -1.8
Or see this region's nucleotide sequence