Strain Fitness in Escherichia coli ECRC62 around BNILDI_12215

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaB and guaA are separated by 68 nucleotidesguaA and yfgI are separated by 94 nucleotidesyfgI and yfgH are separated by 15 nucleotides BNILDI_12210: guaB - IMP dehydrogenase, at 2,451,013 to 2,452,479 guaB BNILDI_12215: guaA - glutamine-hydrolyzing GMP synthase, at 2,452,548 to 2,454,125 guaA BNILDI_12220: yfgI - Uncharacterized protein YfgI, at 2,454,220 to 2,454,759 yfgI BNILDI_12225: yfgH - Uncharacterized lipoprotein YfgH, at 2,454,775 to 2,455,290 yfgH Position (kb) 2452 2453 2454 2455Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2451.810 kb on + strand, within guaBat 2451.921 kb on + strand, within guaBat 2451.921 kb on + strand, within guaBat 2451.922 kb on - strand, within guaBat 2451.927 kb on + strand, within guaBat 2451.942 kb on + strand, within guaBat 2452.142 kb on - strand, within guaBat 2452.275 kb on - strand, within guaBat 2452.279 kb on + strand, within guaBat 2452.281 kb on + strand, within guaBat 2452.282 kb on - strand, within guaBat 2452.335 kb on + strandat 2452.356 kb on + strandat 2452.359 kb on + strandat 2452.378 kb on - strandat 2452.391 kb on + strandat 2452.391 kb on + strandat 2452.392 kb on - strandat 2452.446 kb on + strandat 2452.447 kb on - strandat 2452.603 kb on - strandat 2452.946 kb on + strand, within guaAat 2453.200 kb on + strand, within guaAat 2453.320 kb on + strand, within guaAat 2453.372 kb on + strand, within guaAat 2453.373 kb on - strand, within guaAat 2453.500 kb on + strand, within guaAat 2453.742 kb on + strand, within guaAat 2453.855 kb on + strand, within guaAat 2453.908 kb on + strand, within guaAat 2453.939 kb on + strand, within guaAat 2453.940 kb on - strand, within guaAat 2454.445 kb on + strand, within yfgIat 2454.446 kb on - strand, within yfgIat 2454.722 kb on - strandat 2454.722 kb on - strandat 2454.774 kb on - strandat 2454.777 kb on - strandat 2454.824 kb on + strandat 2454.824 kb on + strandat 2454.825 kb on - strandat 2454.838 kb on - strand, within yfgHat 2454.893 kb on + strand, within yfgHat 2454.894 kb on - strand, within yfgHat 2454.894 kb on - strand, within yfgHat 2454.912 kb on + strand, within yfgHat 2454.912 kb on + strand, within yfgHat 2454.912 kb on + strand, within yfgHat 2454.914 kb on + strand, within yfgHat 2454.938 kb on - strand, within yfgHat 2454.957 kb on + strand, within yfgHat 2454.958 kb on - strand, within yfgH

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,451,810 + guaB BNILDI_12210 0.54 -2.5
2,451,921 + guaB BNILDI_12210 0.62 -4.2
2,451,921 + guaB BNILDI_12210 0.62 -2.6
2,451,922 - guaB BNILDI_12210 0.62 -0.9
2,451,927 + guaB BNILDI_12210 0.62 -0.9
2,451,942 + guaB BNILDI_12210 0.63 -2.4
2,452,142 - guaB BNILDI_12210 0.77 -1.9
2,452,275 - guaB BNILDI_12210 0.86 -2.7
2,452,279 + guaB BNILDI_12210 0.86 -3.7
2,452,281 + guaB BNILDI_12210 0.86 -2.0
2,452,282 - guaB BNILDI_12210 0.87 -2.2
2,452,335 + -2.0
2,452,356 + -4.8
2,452,359 + -4.4
2,452,378 - -2.3
2,452,391 + -1.7
2,452,391 + -3.4
2,452,392 - -0.1
2,452,446 + -0.9
2,452,447 - -2.4
2,452,603 - -0.2
2,452,946 + guaA BNILDI_12215 0.25 -0.7
2,453,200 + guaA BNILDI_12215 0.41 -4.2
2,453,320 + guaA BNILDI_12215 0.49 -3.2
2,453,372 + guaA BNILDI_12215 0.52 -2.0
2,453,373 - guaA BNILDI_12215 0.52 -2.2
2,453,500 + guaA BNILDI_12215 0.60 -2.1
2,453,742 + guaA BNILDI_12215 0.76 -5.2
2,453,855 + guaA BNILDI_12215 0.83 -1.1
2,453,908 + guaA BNILDI_12215 0.86 -1.8
2,453,939 + guaA BNILDI_12215 0.88 -2.5
2,453,940 - guaA BNILDI_12215 0.88 -3.1
2,454,445 + yfgI BNILDI_12220 0.42 -2.1
2,454,446 - yfgI BNILDI_12220 0.42 -1.7
2,454,722 - +0.0
2,454,722 - -0.2
2,454,774 - -0.0
2,454,777 - +1.0
2,454,824 + +0.3
2,454,824 + -0.1
2,454,825 - -0.1
2,454,838 - yfgH BNILDI_12225 0.12 +0.7
2,454,893 + yfgH BNILDI_12225 0.23 +0.8
2,454,894 - yfgH BNILDI_12225 0.23 -0.2
2,454,894 - yfgH BNILDI_12225 0.23 -1.3
2,454,912 + yfgH BNILDI_12225 0.27 -0.9
2,454,912 + yfgH BNILDI_12225 0.27 -0.7
2,454,912 + yfgH BNILDI_12225 0.27 +1.2
2,454,914 + yfgH BNILDI_12225 0.27 -0.1
2,454,938 - yfgH BNILDI_12225 0.32 +2.2
2,454,957 + yfgH BNILDI_12225 0.35 +0.6
2,454,958 - yfgH BNILDI_12225 0.35 +0.5

Or see this region's nucleotide sequence