Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysH are separated by 73 nucleotides cysH and hokG are separated by 264 nucleotides hokG and cas3 are separated by 193 nucleotides
BNILDI_10740: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 2,172,812 to 2,174,524
cysI
BNILDI_10745: cysH - phosphoadenosine phosphosulfate reductase, at 2,174,598 to 2,175,332
cysH
BNILDI_10755: hokG - Putative protein HokG, at 2,175,597 to 2,175,749
hokG
BNILDI_10760: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 2,175,943 to 2,178,642
cas3
Position (kb)
2174
2175
2176 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2173.655 kb on - strand, within cysI at 2173.655 kb on - strand, within cysI at 2173.737 kb on - strand, within cysI at 2173.737 kb on - strand, within cysI at 2173.820 kb on + strand, within cysI at 2173.874 kb on - strand, within cysI at 2174.189 kb on - strand, within cysI at 2174.193 kb on + strand, within cysI at 2174.351 kb on - strand, within cysI at 2174.543 kb on - strand at 2174.597 kb on + strand at 2174.598 kb on - strand at 2174.642 kb on - strand at 2174.740 kb on - strand, within cysH at 2174.740 kb on - strand, within cysH at 2174.743 kb on + strand, within cysH at 2174.744 kb on - strand, within cysH at 2174.744 kb on - strand, within cysH at 2174.744 kb on - strand, within cysH at 2174.988 kb on + strand, within cysH at 2175.128 kb on + strand, within cysH at 2175.541 kb on + strand at 2175.541 kb on + strand at 2175.541 kb on + strand at 2175.569 kb on + strand at 2175.610 kb on - strand at 2175.632 kb on - strand, within hokG at 2175.641 kb on + strand, within hokG at 2175.642 kb on - strand, within hokG at 2175.642 kb on - strand, within hokG at 2175.667 kb on + strand, within hokG at 2175.667 kb on + strand, within hokG at 2175.668 kb on - strand, within hokG at 2176.331 kb on - strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,173,655 - cysI BNILDI_10740 0.49 +0.8 2,173,655 - cysI BNILDI_10740 0.49 -1.6 2,173,737 - cysI BNILDI_10740 0.54 -3.1 2,173,737 - cysI BNILDI_10740 0.54 -4.2 2,173,820 + cysI BNILDI_10740 0.59 -2.2 2,173,874 - cysI BNILDI_10740 0.62 -0.7 2,174,189 - cysI BNILDI_10740 0.80 -1.9 2,174,193 + cysI BNILDI_10740 0.81 -2.1 2,174,351 - cysI BNILDI_10740 0.90 -2.2 2,174,543 - -1.8 2,174,597 + -2.0 2,174,598 - -4.2 2,174,642 - -1.5 2,174,740 - cysH BNILDI_10745 0.19 -1.6 2,174,740 - cysH BNILDI_10745 0.19 -2.3 2,174,743 + cysH BNILDI_10745 0.20 -3.8 2,174,744 - cysH BNILDI_10745 0.20 -4.6 2,174,744 - cysH BNILDI_10745 0.20 +1.4 2,174,744 - cysH BNILDI_10745 0.20 -2.4 2,174,988 + cysH BNILDI_10745 0.53 -5.7 2,175,128 + cysH BNILDI_10745 0.72 -4.0 2,175,541 + +0.5 2,175,541 + +0.7 2,175,541 + +1.0 2,175,569 + -0.1 2,175,610 - +0.8 2,175,632 - hokG BNILDI_10755 0.23 -0.6 2,175,641 + hokG BNILDI_10755 0.29 +0.2 2,175,642 - hokG BNILDI_10755 0.29 +0.2 2,175,642 - hokG BNILDI_10755 0.29 +0.2 2,175,667 + hokG BNILDI_10755 0.46 +0.1 2,175,667 + hokG BNILDI_10755 0.46 +0.5 2,175,668 - hokG BNILDI_10755 0.46 -0.3 2,176,331 - cas3 BNILDI_10760 0.14 -0.1
Or see this region's nucleotide sequence