Strain Fitness in Escherichia coli ECRC62 around BNILDI_10745

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysI and cysH are separated by 73 nucleotidescysH and hokG are separated by 264 nucleotideshokG and cas3 are separated by 193 nucleotides BNILDI_10740: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 2,172,812 to 2,174,524 cysI BNILDI_10745: cysH - phosphoadenosine phosphosulfate reductase, at 2,174,598 to 2,175,332 cysH BNILDI_10755: hokG - Putative protein HokG, at 2,175,597 to 2,175,749 hokG BNILDI_10760: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 2,175,943 to 2,178,642 cas3 Position (kb) 2174 2175 2176Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2173.655 kb on - strand, within cysIat 2173.655 kb on - strand, within cysIat 2173.737 kb on - strand, within cysIat 2173.737 kb on - strand, within cysIat 2173.820 kb on + strand, within cysIat 2173.874 kb on - strand, within cysIat 2174.189 kb on - strand, within cysIat 2174.193 kb on + strand, within cysIat 2174.351 kb on - strand, within cysIat 2174.543 kb on - strandat 2174.597 kb on + strandat 2174.598 kb on - strandat 2174.642 kb on - strandat 2174.740 kb on - strand, within cysHat 2174.740 kb on - strand, within cysHat 2174.743 kb on + strand, within cysHat 2174.744 kb on - strand, within cysHat 2174.744 kb on - strand, within cysHat 2174.744 kb on - strand, within cysHat 2174.988 kb on + strand, within cysHat 2175.128 kb on + strand, within cysHat 2175.541 kb on + strandat 2175.541 kb on + strandat 2175.541 kb on + strandat 2175.569 kb on + strandat 2175.610 kb on - strandat 2175.632 kb on - strand, within hokGat 2175.641 kb on + strand, within hokGat 2175.642 kb on - strand, within hokGat 2175.642 kb on - strand, within hokGat 2175.667 kb on + strand, within hokGat 2175.667 kb on + strand, within hokGat 2175.668 kb on - strand, within hokGat 2176.331 kb on - strand, within cas3

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,173,655 - cysI BNILDI_10740 0.49 +0.8
2,173,655 - cysI BNILDI_10740 0.49 -1.6
2,173,737 - cysI BNILDI_10740 0.54 -3.1
2,173,737 - cysI BNILDI_10740 0.54 -4.2
2,173,820 + cysI BNILDI_10740 0.59 -2.2
2,173,874 - cysI BNILDI_10740 0.62 -0.7
2,174,189 - cysI BNILDI_10740 0.80 -1.9
2,174,193 + cysI BNILDI_10740 0.81 -2.1
2,174,351 - cysI BNILDI_10740 0.90 -2.2
2,174,543 - -1.8
2,174,597 + -2.0
2,174,598 - -4.2
2,174,642 - -1.5
2,174,740 - cysH BNILDI_10745 0.19 -1.6
2,174,740 - cysH BNILDI_10745 0.19 -2.3
2,174,743 + cysH BNILDI_10745 0.20 -3.8
2,174,744 - cysH BNILDI_10745 0.20 -4.6
2,174,744 - cysH BNILDI_10745 0.20 +1.4
2,174,744 - cysH BNILDI_10745 0.20 -2.4
2,174,988 + cysH BNILDI_10745 0.53 -5.7
2,175,128 + cysH BNILDI_10745 0.72 -4.0
2,175,541 + +0.5
2,175,541 + +0.7
2,175,541 + +1.0
2,175,569 + -0.1
2,175,610 - +0.8
2,175,632 - hokG BNILDI_10755 0.23 -0.6
2,175,641 + hokG BNILDI_10755 0.29 +0.2
2,175,642 - hokG BNILDI_10755 0.29 +0.2
2,175,642 - hokG BNILDI_10755 0.29 +0.2
2,175,667 + hokG BNILDI_10755 0.46 +0.1
2,175,667 + hokG BNILDI_10755 0.46 +0.5
2,175,668 - hokG BNILDI_10755 0.46 -0.3
2,176,331 - cas3 BNILDI_10760 0.14 -0.1

Or see this region's nucleotide sequence