Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygcN and queD are separated by 77 nucleotides queD and cysJ are separated by 315 nucleotides cysJ and cysI overlap by 1 nucleotides
BNILDI_10725: ygcN - putative electron transfer flavoprotein-quinone oxidoreductase YgcN, at 2,168,983 to 2,170,254
ygcN
BNILDI_10730: queD - 6-carboxytetrahydropterin synthase QueD, at 2,170,332 to 2,170,697
queD
BNILDI_10735: cysJ - NADPH-dependent assimilatory sulfite reductase flavoprotein subunit, at 2,171,013 to 2,172,812
cysJ
BNILDI_10740: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 2,172,812 to 2,174,524
cysI
Position (kb)
2171
2172
2173 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2170.104 kb on + strand, within ygcN at 2170.104 kb on + strand, within ygcN at 2170.105 kb on - strand, within ygcN at 2170.105 kb on - strand, within ygcN at 2170.106 kb on + strand, within ygcN at 2170.106 kb on + strand, within ygcN at 2170.106 kb on + strand, within ygcN at 2170.107 kb on - strand, within ygcN at 2170.107 kb on - strand, within ygcN at 2170.107 kb on - strand, within ygcN at 2170.108 kb on + strand, within ygcN at 2170.108 kb on + strand, within ygcN at 2170.109 kb on - strand, within ygcN at 2170.228 kb on - strand at 2170.228 kb on - strand at 2170.231 kb on - strand at 2170.231 kb on - strand at 2170.231 kb on - strand at 2170.231 kb on - strand at 2170.233 kb on + strand at 2170.234 kb on - strand at 2170.331 kb on - strand at 2170.331 kb on - strand at 2170.331 kb on - strand at 2170.374 kb on + strand, within queD at 2170.389 kb on + strand, within queD at 2170.390 kb on - strand, within queD at 2170.390 kb on - strand, within queD at 2170.390 kb on - strand, within queD at 2170.390 kb on - strand, within queD at 2170.390 kb on - strand, within queD at 2170.480 kb on + strand, within queD at 2170.501 kb on + strand, within queD at 2170.502 kb on - strand, within queD at 2170.502 kb on - strand, within queD at 2170.512 kb on - strand, within queD at 2170.593 kb on - strand, within queD at 2170.622 kb on + strand, within queD at 2170.623 kb on - strand, within queD at 2170.680 kb on + strand at 2170.681 kb on - strand at 2170.697 kb on + strand at 2170.728 kb on - strand at 2170.758 kb on - strand at 2170.894 kb on - strand at 2170.943 kb on - strand at 2170.960 kb on - strand at 2171.005 kb on - strand at 2171.203 kb on + strand, within cysJ at 2171.204 kb on - strand, within cysJ at 2171.207 kb on + strand, within cysJ at 2171.207 kb on + strand, within cysJ at 2171.208 kb on - strand, within cysJ at 2171.270 kb on - strand, within cysJ at 2171.273 kb on - strand, within cysJ at 2171.408 kb on + strand, within cysJ at 2171.409 kb on - strand, within cysJ at 2171.580 kb on + strand, within cysJ at 2171.669 kb on + strand, within cysJ at 2171.763 kb on - strand, within cysJ at 2171.763 kb on - strand, within cysJ at 2171.801 kb on - strand, within cysJ at 2171.801 kb on - strand, within cysJ at 2172.065 kb on - strand, within cysJ at 2172.214 kb on + strand, within cysJ at 2172.215 kb on - strand, within cysJ at 2172.336 kb on - strand, within cysJ at 2172.342 kb on - strand, within cysJ at 2172.381 kb on - strand, within cysJ at 2172.381 kb on - strand, within cysJ at 2172.422 kb on + strand, within cysJ at 2172.525 kb on + strand, within cysJ at 2172.525 kb on + strand, within cysJ at 2172.551 kb on + strand, within cysJ at 2172.596 kb on - strand, within cysJ at 2172.660 kb on + strand at 2172.660 kb on + strand at 2172.661 kb on - strand at 2172.661 kb on - strand at 2172.781 kb on + strand at 2172.808 kb on - strand at 2172.810 kb on + strand at 2172.811 kb on - strand at 2172.811 kb on - strand at 2172.890 kb on + strand at 2172.890 kb on + strand at 2172.891 kb on - strand at 2172.983 kb on + strand at 2172.984 kb on - strand, within cysI at 2172.984 kb on - strand, within cysI at 2172.984 kb on - strand, within cysI at 2172.984 kb on - strand, within cysI at 2173.136 kb on + strand, within cysI at 2173.136 kb on + strand, within cysI at 2173.136 kb on + strand, within cysI at 2173.137 kb on - strand, within cysI at 2173.137 kb on - strand, within cysI at 2173.137 kb on - strand, within cysI at 2173.271 kb on - strand, within cysI at 2173.276 kb on - strand, within cysI at 2173.276 kb on - strand, within cysI at 2173.276 kb on - strand, within cysI at 2173.411 kb on + strand, within cysI at 2173.471 kb on - strand, within cysI at 2173.586 kb on - strand, within cysI at 2173.655 kb on - strand, within cysI at 2173.655 kb on - strand, within cysI at 2173.737 kb on - strand, within cysI at 2173.737 kb on - strand, within cysI
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,170,104 + ygcN BNILDI_10725 0.88 -0.1 2,170,104 + ygcN BNILDI_10725 0.88 +0.8 2,170,105 - ygcN BNILDI_10725 0.88 +0.9 2,170,105 - ygcN BNILDI_10725 0.88 -0.0 2,170,106 + ygcN BNILDI_10725 0.88 +2.3 2,170,106 + ygcN BNILDI_10725 0.88 +0.1 2,170,106 + ygcN BNILDI_10725 0.88 -1.4 2,170,107 - ygcN BNILDI_10725 0.88 -1.5 2,170,107 - ygcN BNILDI_10725 0.88 +0.0 2,170,107 - ygcN BNILDI_10725 0.88 +0.8 2,170,108 + ygcN BNILDI_10725 0.88 -0.4 2,170,108 + ygcN BNILDI_10725 0.88 +0.2 2,170,109 - ygcN BNILDI_10725 0.89 -0.4 2,170,228 - -0.4 2,170,228 - -0.4 2,170,231 - +0.9 2,170,231 - -0.6 2,170,231 - +0.8 2,170,231 - -0.9 2,170,233 + +1.0 2,170,234 - -2.9 2,170,331 - +2.3 2,170,331 - +0.1 2,170,331 - +0.9 2,170,374 + queD BNILDI_10730 0.11 -0.2 2,170,389 + queD BNILDI_10730 0.16 +0.1 2,170,390 - queD BNILDI_10730 0.16 +2.5 2,170,390 - queD BNILDI_10730 0.16 +1.2 2,170,390 - queD BNILDI_10730 0.16 +0.2 2,170,390 - queD BNILDI_10730 0.16 -0.1 2,170,390 - queD BNILDI_10730 0.16 +0.1 2,170,480 + queD BNILDI_10730 0.40 -0.6 2,170,501 + queD BNILDI_10730 0.46 -0.1 2,170,502 - queD BNILDI_10730 0.46 +0.2 2,170,502 - queD BNILDI_10730 0.46 +0.3 2,170,512 - queD BNILDI_10730 0.49 -1.5 2,170,593 - queD BNILDI_10730 0.71 -3.3 2,170,622 + queD BNILDI_10730 0.79 -1.0 2,170,623 - queD BNILDI_10730 0.80 +0.2 2,170,680 + -0.8 2,170,681 - +0.2 2,170,697 + +0.1 2,170,728 - +2.3 2,170,758 - -1.3 2,170,894 - -4.2 2,170,943 - -1.0 2,170,960 - -3.4 2,171,005 - -3.4 2,171,203 + cysJ BNILDI_10735 0.11 -1.0 2,171,204 - cysJ BNILDI_10735 0.11 -2.5 2,171,207 + cysJ BNILDI_10735 0.11 -2.4 2,171,207 + cysJ BNILDI_10735 0.11 -1.3 2,171,208 - cysJ BNILDI_10735 0.11 -3.7 2,171,270 - cysJ BNILDI_10735 0.14 -2.5 2,171,273 - cysJ BNILDI_10735 0.14 -3.4 2,171,408 + cysJ BNILDI_10735 0.22 -2.7 2,171,409 - cysJ BNILDI_10735 0.22 -3.5 2,171,580 + cysJ BNILDI_10735 0.32 -2.6 2,171,669 + cysJ BNILDI_10735 0.36 -3.5 2,171,763 - cysJ BNILDI_10735 0.42 -3.5 2,171,763 - cysJ BNILDI_10735 0.42 -3.0 2,171,801 - cysJ BNILDI_10735 0.44 -3.2 2,171,801 - cysJ BNILDI_10735 0.44 -3.1 2,172,065 - cysJ BNILDI_10735 0.58 -1.8 2,172,214 + cysJ BNILDI_10735 0.67 -3.8 2,172,215 - cysJ BNILDI_10735 0.67 -4.0 2,172,336 - cysJ BNILDI_10735 0.73 -2.1 2,172,342 - cysJ BNILDI_10735 0.74 -3.0 2,172,381 - cysJ BNILDI_10735 0.76 -0.9 2,172,381 - cysJ BNILDI_10735 0.76 -2.6 2,172,422 + cysJ BNILDI_10735 0.78 -2.3 2,172,525 + cysJ BNILDI_10735 0.84 -2.4 2,172,525 + cysJ BNILDI_10735 0.84 -1.8 2,172,551 + cysJ BNILDI_10735 0.85 -2.6 2,172,596 - cysJ BNILDI_10735 0.88 -1.6 2,172,660 + -2.3 2,172,660 + -1.4 2,172,661 - -4.1 2,172,661 - -2.5 2,172,781 + -1.9 2,172,808 - -4.7 2,172,810 + -0.4 2,172,811 - -1.9 2,172,811 - -2.3 2,172,890 + -3.4 2,172,890 + -2.8 2,172,891 - -1.4 2,172,983 + -2.2 2,172,984 - cysI BNILDI_10740 0.10 -3.9 2,172,984 - cysI BNILDI_10740 0.10 +0.1 2,172,984 - cysI BNILDI_10740 0.10 -2.4 2,172,984 - cysI BNILDI_10740 0.10 -3.4 2,173,136 + cysI BNILDI_10740 0.19 -2.4 2,173,136 + cysI BNILDI_10740 0.19 -2.0 2,173,136 + cysI BNILDI_10740 0.19 -2.6 2,173,137 - cysI BNILDI_10740 0.19 -3.7 2,173,137 - cysI BNILDI_10740 0.19 -3.1 2,173,137 - cysI BNILDI_10740 0.19 -3.5 2,173,271 - cysI BNILDI_10740 0.27 -2.0 2,173,276 - cysI BNILDI_10740 0.27 -2.1 2,173,276 - cysI BNILDI_10740 0.27 -3.8 2,173,276 - cysI BNILDI_10740 0.27 -1.6 2,173,411 + cysI BNILDI_10740 0.35 -1.3 2,173,471 - cysI BNILDI_10740 0.38 -3.1 2,173,586 - cysI BNILDI_10740 0.45 -1.9 2,173,655 - cysI BNILDI_10740 0.49 +0.8 2,173,655 - cysI BNILDI_10740 0.49 -1.6 2,173,737 - cysI BNILDI_10740 0.54 -3.1 2,173,737 - cysI BNILDI_10740 0.54 -4.2
Or see this region's nucleotide sequence