Strain Fitness in Escherichia coli ECRC62 around BNILDI_10540

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfucI and fucP are separated by 32 nucleotidesfucP and fucA are separated by 546 nucleotides BNILDI_10535: fucI - L-fucose isomerase, at 2,124,235 to 2,126,010 fucI BNILDI_10540: fucP - L-fucose:H+ symporter permease, at 2,126,043 to 2,127,359 fucP BNILDI_10545: fucA - L-fuculose-phosphate aldolase, at 2,127,906 to 2,128,553 fucA Position (kb) 2126 2127 2128Strain fitness (log2 ratio) -3 -2 -1 0 1at 2125.481 kb on + strand, within fucIat 2125.481 kb on + strand, within fucIat 2125.482 kb on - strand, within fucIat 2125.488 kb on + strand, within fucIat 2125.489 kb on - strand, within fucIat 2125.489 kb on - strand, within fucIat 2125.827 kb on + strand, within fucIat 2125.915 kb on - strandat 2126.028 kb on - strandat 2126.049 kb on - strandat 2126.196 kb on - strand, within fucPat 2126.216 kb on - strand, within fucPat 2126.241 kb on - strand, within fucPat 2126.280 kb on - strand, within fucPat 2126.292 kb on + strand, within fucPat 2126.292 kb on + strand, within fucPat 2126.304 kb on + strand, within fucPat 2126.305 kb on - strand, within fucPat 2126.369 kb on - strand, within fucPat 2126.461 kb on + strand, within fucPat 2126.461 kb on + strand, within fucPat 2126.462 kb on - strand, within fucPat 2126.502 kb on + strand, within fucPat 2126.503 kb on - strand, within fucPat 2126.503 kb on - strand, within fucPat 2126.509 kb on + strand, within fucPat 2126.522 kb on - strand, within fucPat 2126.522 kb on - strand, within fucPat 2126.565 kb on + strand, within fucPat 2126.568 kb on + strand, within fucPat 2126.649 kb on - strand, within fucPat 2126.700 kb on + strand, within fucPat 2126.723 kb on + strand, within fucPat 2126.723 kb on + strand, within fucPat 2126.724 kb on - strand, within fucPat 2126.725 kb on + strand, within fucPat 2126.725 kb on + strand, within fucPat 2126.726 kb on - strand, within fucPat 2126.726 kb on - strand, within fucPat 2126.737 kb on - strand, within fucPat 2126.759 kb on + strand, within fucPat 2126.759 kb on + strand, within fucPat 2127.315 kb on - strandat 2127.385 kb on - strandat 2127.547 kb on - strandat 2127.904 kb on + strandat 2127.918 kb on - strandat 2128.001 kb on - strand, within fucAat 2128.068 kb on - strand, within fucAat 2128.243 kb on - strand, within fucAat 2128.243 kb on - strand, within fucAat 2128.266 kb on - strand, within fucAat 2128.266 kb on - strand, within fucAat 2128.271 kb on + strand, within fucA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,125,481 + fucI BNILDI_10535 0.70 -0.3
2,125,481 + fucI BNILDI_10535 0.70 -0.6
2,125,482 - fucI BNILDI_10535 0.70 +0.7
2,125,488 + fucI BNILDI_10535 0.71 +1.1
2,125,489 - fucI BNILDI_10535 0.71 +1.3
2,125,489 - fucI BNILDI_10535 0.71 -0.8
2,125,827 + fucI BNILDI_10535 0.90 +0.3
2,125,915 - +0.4
2,126,028 - -3.0
2,126,049 - +0.5
2,126,196 - fucP BNILDI_10540 0.12 -0.9
2,126,216 - fucP BNILDI_10540 0.13 +0.7
2,126,241 - fucP BNILDI_10540 0.15 -0.8
2,126,280 - fucP BNILDI_10540 0.18 +0.0
2,126,292 + fucP BNILDI_10540 0.19 -0.5
2,126,292 + fucP BNILDI_10540 0.19 +0.3
2,126,304 + fucP BNILDI_10540 0.20 -0.2
2,126,305 - fucP BNILDI_10540 0.20 +0.5
2,126,369 - fucP BNILDI_10540 0.25 +0.0
2,126,461 + fucP BNILDI_10540 0.32 -0.2
2,126,461 + fucP BNILDI_10540 0.32 -0.3
2,126,462 - fucP BNILDI_10540 0.32 -0.3
2,126,502 + fucP BNILDI_10540 0.35 +1.0
2,126,503 - fucP BNILDI_10540 0.35 +0.3
2,126,503 - fucP BNILDI_10540 0.35 -1.1
2,126,509 + fucP BNILDI_10540 0.35 +0.7
2,126,522 - fucP BNILDI_10540 0.36 -0.0
2,126,522 - fucP BNILDI_10540 0.36 -0.3
2,126,565 + fucP BNILDI_10540 0.40 -0.9
2,126,568 + fucP BNILDI_10540 0.40 +0.3
2,126,649 - fucP BNILDI_10540 0.46 +0.2
2,126,700 + fucP BNILDI_10540 0.50 -2.7
2,126,723 + fucP BNILDI_10540 0.52 -0.0
2,126,723 + fucP BNILDI_10540 0.52 +1.1
2,126,724 - fucP BNILDI_10540 0.52 +0.7
2,126,725 + fucP BNILDI_10540 0.52 +0.1
2,126,725 + fucP BNILDI_10540 0.52 +1.1
2,126,726 - fucP BNILDI_10540 0.52 +1.7
2,126,726 - fucP BNILDI_10540 0.52 -0.7
2,126,737 - fucP BNILDI_10540 0.53 -0.9
2,126,759 + fucP BNILDI_10540 0.54 +0.5
2,126,759 + fucP BNILDI_10540 0.54 +0.1
2,127,315 - +0.7
2,127,385 - +1.2
2,127,547 - +0.1
2,127,904 + -0.7
2,127,918 - +0.0
2,128,001 - fucA BNILDI_10545 0.15 -2.3
2,128,068 - fucA BNILDI_10545 0.25 -0.5
2,128,243 - fucA BNILDI_10545 0.52 +1.3
2,128,243 - fucA BNILDI_10545 0.52 +0.5
2,128,266 - fucA BNILDI_10545 0.56 -0.8
2,128,266 - fucA BNILDI_10545 0.56 -1.9
2,128,271 + fucA BNILDI_10545 0.56 +0.6

Or see this region's nucleotide sequence