Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptrA and recB overlap by 8 nucleotides recB and recD overlap by 1 nucleotides
BNILDI_10410: ptrA - pitrilysin, at 2,097,036 to 2,099,924
ptrA
BNILDI_10415: recB - exodeoxyribonuclease V subunit beta, at 2,099,917 to 2,103,459
recB
BNILDI_10420: recD - exodeoxyribonuclease V subunit alpha, at 2,103,459 to 2,105,285
recD
Position (kb)
2099
2100
2101
2102
2103
2104 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2099.082 kb on - strand, within ptrA at 2099.141 kb on + strand, within ptrA at 2099.142 kb on - strand, within ptrA at 2099.289 kb on - strand, within ptrA at 2099.344 kb on - strand, within ptrA at 2099.358 kb on + strand, within ptrA at 2099.361 kb on - strand, within ptrA at 2099.361 kb on - strand, within ptrA at 2099.384 kb on - strand, within ptrA at 2099.663 kb on - strand at 2099.663 kb on - strand at 2099.687 kb on + strand at 2099.709 kb on - strand at 2099.838 kb on + strand at 2099.839 kb on - strand at 2099.839 kb on - strand at 2099.839 kb on - strand at 2099.940 kb on - strand at 2100.025 kb on + strand at 2101.173 kb on - strand, within recB at 2101.207 kb on - strand, within recB at 2101.464 kb on + strand, within recB at 2102.595 kb on - strand, within recB at 2103.584 kb on - strand at 2103.638 kb on + strand at 2103.638 kb on + strand at 2103.781 kb on - strand, within recD at 2103.800 kb on + strand, within recD at 2103.801 kb on - strand, within recD at 2103.911 kb on + strand, within recD at 2103.986 kb on + strand, within recD at 2104.021 kb on + strand, within recD at 2104.021 kb on + strand, within recD at 2104.127 kb on - strand, within recD at 2104.127 kb on - strand, within recD at 2104.289 kb on + strand, within recD at 2104.404 kb on + strand, within recD at 2104.404 kb on + strand, within recD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,099,082 - ptrA BNILDI_10410 0.71 -0.4 2,099,141 + ptrA BNILDI_10410 0.73 +0.1 2,099,142 - ptrA BNILDI_10410 0.73 +0.5 2,099,289 - ptrA BNILDI_10410 0.78 +0.1 2,099,344 - ptrA BNILDI_10410 0.80 +0.0 2,099,358 + ptrA BNILDI_10410 0.80 +0.5 2,099,361 - ptrA BNILDI_10410 0.80 -0.3 2,099,361 - ptrA BNILDI_10410 0.80 +0.1 2,099,384 - ptrA BNILDI_10410 0.81 +0.5 2,099,663 - -2.0 2,099,663 - -0.1 2,099,687 + -0.8 2,099,709 - -0.6 2,099,838 + +0.1 2,099,839 - -0.6 2,099,839 - -0.6 2,099,839 - -0.2 2,099,940 - -1.1 2,100,025 + -0.3 2,101,173 - recB BNILDI_10415 0.35 +0.4 2,101,207 - recB BNILDI_10415 0.36 -0.6 2,101,464 + recB BNILDI_10415 0.44 -2.2 2,102,595 - recB BNILDI_10415 0.76 -3.8 2,103,584 - -1.1 2,103,638 + +1.0 2,103,638 + +0.2 2,103,781 - recD BNILDI_10420 0.18 -1.7 2,103,800 + recD BNILDI_10420 0.19 -0.6 2,103,801 - recD BNILDI_10420 0.19 +1.2 2,103,911 + recD BNILDI_10420 0.25 +0.7 2,103,986 + recD BNILDI_10420 0.29 +0.5 2,104,021 + recD BNILDI_10420 0.31 -0.6 2,104,021 + recD BNILDI_10420 0.31 -0.1 2,104,127 - recD BNILDI_10420 0.37 +0.3 2,104,127 - recD BNILDI_10420 0.37 -0.2 2,104,289 + recD BNILDI_10420 0.45 +0.1 2,104,404 + recD BNILDI_10420 0.52 +0.1 2,104,404 + recD BNILDI_10420 0.52 -0.1
Or see this region's nucleotide sequence