Strain Fitness in Escherichia coli ECRC62 around BNILDI_10025

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygfX and fldB are separated by 39 nucleotidesfldB and xerD are separated by 111 nucleotidesxerD and dsbC are separated by 24 nucleotidesdsbC and recJ are separated by 5 nucleotides BNILDI_10015: ygfX - Inner membrane protein YgfX, at 2,010,899 to 2,011,306 ygfX BNILDI_10020: fldB - flavodoxin FldB, at 2,011,346 to 2,011,867 fldB BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875 xerD BNILDI_10030: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 2,012,900 to 2,013,610 dsbC BNILDI_10035: recJ - single-stranded-DNA-specific exonuclease RecJ, at 2,013,616 to 2,015,349 recJ Position (kb) 2011 2012 2013Strain fitness (log2 ratio) -3 -2 -1 0 1at 2010.982 kb on + strand, within ygfXat 2010.988 kb on - strand, within ygfXat 2010.988 kb on - strand, within ygfXat 2011.011 kb on - strand, within ygfXat 2011.076 kb on - strand, within ygfXat 2011.078 kb on + strand, within ygfXat 2011.078 kb on + strand, within ygfXat 2011.172 kb on - strand, within ygfXat 2011.172 kb on - strand, within ygfXat 2011.194 kb on + strand, within ygfXat 2011.194 kb on + strand, within ygfXat 2011.224 kb on + strand, within ygfXat 2011.224 kb on + strand, within ygfXat 2011.225 kb on - strand, within ygfXat 2011.323 kb on + strandat 2011.324 kb on - strandat 2011.353 kb on + strandat 2011.353 kb on + strandat 2011.399 kb on + strand, within fldBat 2011.400 kb on - strand, within fldBat 2011.427 kb on - strand, within fldBat 2011.731 kb on + strand, within fldBat 2011.732 kb on - strand, within fldBat 2011.742 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.773 kb on - strand, within fldBat 2011.796 kb on - strand, within fldBat 2011.831 kb on - strandat 2011.861 kb on + strandat 2011.905 kb on + strandat 2011.906 kb on - strandat 2011.910 kb on + strandat 2011.924 kb on + strandat 2011.946 kb on - strandat 2011.967 kb on + strandat 2012.050 kb on + strandat 2012.405 kb on + strand, within xerDat 2012.405 kb on + strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.739 kb on + strand, within xerDat 2012.914 kb on + strandat 2012.914 kb on + strandat 2012.915 kb on - strandat 2012.915 kb on - strandat 2013.071 kb on + strand, within dsbCat 2013.072 kb on - strand, within dsbCat 2013.072 kb on - strand, within dsbCat 2013.094 kb on + strand, within dsbCat 2013.095 kb on - strand, within dsbCat 2013.095 kb on - strand, within dsbCat 2013.113 kb on + strand, within dsbCat 2013.113 kb on + strand, within dsbCat 2013.114 kb on - strand, within dsbCat 2013.244 kb on + strand, within dsbCat 2013.245 kb on - strand, within dsbCat 2013.247 kb on + strand, within dsbCat 2013.248 kb on - strand, within dsbCat 2013.292 kb on + strand, within dsbCat 2013.373 kb on + strand, within dsbCat 2013.424 kb on + strand, within dsbCat 2013.425 kb on - strand, within dsbCat 2013.457 kb on + strand, within dsbCat 2013.630 kb on - strandat 2013.757 kb on - strandat 2013.757 kb on - strandat 2013.808 kb on + strand, within recJat 2013.808 kb on + strand, within recJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,010,982 + ygfX BNILDI_10015 0.20 -0.6
2,010,988 - ygfX BNILDI_10015 0.22 +0.8
2,010,988 - ygfX BNILDI_10015 0.22 -0.1
2,011,011 - ygfX BNILDI_10015 0.27 +0.8
2,011,076 - ygfX BNILDI_10015 0.43 -1.7
2,011,078 + ygfX BNILDI_10015 0.44 +0.8
2,011,078 + ygfX BNILDI_10015 0.44 -0.1
2,011,172 - ygfX BNILDI_10015 0.67 -2.1
2,011,172 - ygfX BNILDI_10015 0.67 +1.0
2,011,194 + ygfX BNILDI_10015 0.72 -0.0
2,011,194 + ygfX BNILDI_10015 0.72 +0.5
2,011,224 + ygfX BNILDI_10015 0.80 -0.7
2,011,224 + ygfX BNILDI_10015 0.80 -1.9
2,011,225 - ygfX BNILDI_10015 0.80 -0.8
2,011,323 + +0.1
2,011,324 - +0.2
2,011,353 + -0.1
2,011,353 + +1.1
2,011,399 + fldB BNILDI_10020 0.10 -0.5
2,011,400 - fldB BNILDI_10020 0.10 -0.9
2,011,427 - fldB BNILDI_10020 0.16 -0.7
2,011,731 + fldB BNILDI_10020 0.74 -0.7
2,011,732 - fldB BNILDI_10020 0.74 -2.1
2,011,742 + fldB BNILDI_10020 0.76 -0.5
2,011,772 + fldB BNILDI_10020 0.82 -0.6
2,011,772 + fldB BNILDI_10020 0.82 -1.5
2,011,772 + fldB BNILDI_10020 0.82 +0.1
2,011,772 + fldB BNILDI_10020 0.82 -0.1
2,011,772 + fldB BNILDI_10020 0.82 -0.7
2,011,773 - fldB BNILDI_10020 0.82 -1.9
2,011,796 - fldB BNILDI_10020 0.86 -0.4
2,011,831 - -0.6
2,011,861 + -0.3
2,011,905 + +1.1
2,011,906 - -1.5
2,011,910 + -0.3
2,011,924 + +0.2
2,011,946 - +0.6
2,011,967 + +0.2
2,012,050 + +1.4
2,012,405 + xerD BNILDI_10025 0.47 -0.2
2,012,405 + xerD BNILDI_10025 0.47 -0.3
2,012,406 - xerD BNILDI_10025 0.48 +0.7
2,012,406 - xerD BNILDI_10025 0.48 -1.2
2,012,406 - xerD BNILDI_10025 0.48 -1.1
2,012,739 + xerD BNILDI_10025 0.85 -2.8
2,012,914 + +0.6
2,012,914 + +1.1
2,012,915 - -0.6
2,012,915 - -0.1
2,013,071 + dsbC BNILDI_10030 0.24 -0.3
2,013,072 - dsbC BNILDI_10030 0.24 +0.8
2,013,072 - dsbC BNILDI_10030 0.24 +0.5
2,013,094 + dsbC BNILDI_10030 0.27 -0.6
2,013,095 - dsbC BNILDI_10030 0.27 +0.1
2,013,095 - dsbC BNILDI_10030 0.27 -0.1
2,013,113 + dsbC BNILDI_10030 0.30 +0.1
2,013,113 + dsbC BNILDI_10030 0.30 -0.2
2,013,114 - dsbC BNILDI_10030 0.30 +0.0
2,013,244 + dsbC BNILDI_10030 0.48 -0.2
2,013,245 - dsbC BNILDI_10030 0.49 -0.3
2,013,247 + dsbC BNILDI_10030 0.49 -0.4
2,013,248 - dsbC BNILDI_10030 0.49 -0.1
2,013,292 + dsbC BNILDI_10030 0.55 +0.2
2,013,373 + dsbC BNILDI_10030 0.67 -0.2
2,013,424 + dsbC BNILDI_10030 0.74 +0.5
2,013,425 - dsbC BNILDI_10030 0.74 +0.3
2,013,457 + dsbC BNILDI_10030 0.78 +1.0
2,013,630 - -0.3
2,013,757 - +0.1
2,013,757 - +0.9
2,013,808 + recJ BNILDI_10035 0.11 -0.2
2,013,808 + recJ BNILDI_10035 0.11 +0.2

Or see this region's nucleotide sequence