Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfX and fldB are separated by 39 nucleotides fldB and xerD are separated by 111 nucleotides xerD and dsbC are separated by 24 nucleotides dsbC and recJ are separated by 5 nucleotides
BNILDI_10015: ygfX - Inner membrane protein YgfX, at 2,010,899 to 2,011,306
ygfX
BNILDI_10020: fldB - flavodoxin FldB, at 2,011,346 to 2,011,867
fldB
BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875
xerD
BNILDI_10030: dsbC - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 2,012,900 to 2,013,610
dsbC
BNILDI_10035: recJ - single-stranded-DNA-specific exonuclease RecJ, at 2,013,616 to 2,015,349
recJ
Position (kb)
2011
2012
2013 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2010.982 kb on + strand, within ygfX at 2010.988 kb on - strand, within ygfX at 2010.988 kb on - strand, within ygfX at 2011.011 kb on - strand, within ygfX at 2011.076 kb on - strand, within ygfX at 2011.078 kb on + strand, within ygfX at 2011.078 kb on + strand, within ygfX at 2011.172 kb on - strand, within ygfX at 2011.172 kb on - strand, within ygfX at 2011.194 kb on + strand, within ygfX at 2011.194 kb on + strand, within ygfX at 2011.224 kb on + strand, within ygfX at 2011.224 kb on + strand, within ygfX at 2011.225 kb on - strand, within ygfX at 2011.323 kb on + strand at 2011.324 kb on - strand at 2011.353 kb on + strand at 2011.353 kb on + strand at 2011.399 kb on + strand, within fldB at 2011.400 kb on - strand, within fldB at 2011.427 kb on - strand, within fldB at 2011.731 kb on + strand, within fldB at 2011.732 kb on - strand, within fldB at 2011.742 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.773 kb on - strand, within fldB at 2011.796 kb on - strand, within fldB at 2011.831 kb on - strand at 2011.861 kb on + strand at 2011.905 kb on + strand at 2011.906 kb on - strand at 2011.910 kb on + strand at 2011.924 kb on + strand at 2011.946 kb on - strand at 2011.967 kb on + strand at 2012.050 kb on + strand at 2012.405 kb on + strand, within xerD at 2012.405 kb on + strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.739 kb on + strand, within xerD at 2012.914 kb on + strand at 2012.914 kb on + strand at 2012.915 kb on - strand at 2012.915 kb on - strand at 2013.071 kb on + strand, within dsbC at 2013.072 kb on - strand, within dsbC at 2013.072 kb on - strand, within dsbC at 2013.094 kb on + strand, within dsbC at 2013.095 kb on - strand, within dsbC at 2013.095 kb on - strand, within dsbC at 2013.113 kb on + strand, within dsbC at 2013.113 kb on + strand, within dsbC at 2013.114 kb on - strand, within dsbC at 2013.244 kb on + strand, within dsbC at 2013.245 kb on - strand, within dsbC at 2013.247 kb on + strand, within dsbC at 2013.248 kb on - strand, within dsbC at 2013.292 kb on + strand, within dsbC at 2013.373 kb on + strand, within dsbC at 2013.424 kb on + strand, within dsbC at 2013.425 kb on - strand, within dsbC at 2013.457 kb on + strand, within dsbC at 2013.630 kb on - strand at 2013.757 kb on - strand at 2013.757 kb on - strand at 2013.808 kb on + strand, within recJ at 2013.808 kb on + strand, within recJ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 2,010,982 + ygfX BNILDI_10015 0.20 -0.6 2,010,988 - ygfX BNILDI_10015 0.22 +0.8 2,010,988 - ygfX BNILDI_10015 0.22 -0.1 2,011,011 - ygfX BNILDI_10015 0.27 +0.8 2,011,076 - ygfX BNILDI_10015 0.43 -1.7 2,011,078 + ygfX BNILDI_10015 0.44 +0.8 2,011,078 + ygfX BNILDI_10015 0.44 -0.1 2,011,172 - ygfX BNILDI_10015 0.67 -2.1 2,011,172 - ygfX BNILDI_10015 0.67 +1.0 2,011,194 + ygfX BNILDI_10015 0.72 -0.0 2,011,194 + ygfX BNILDI_10015 0.72 +0.5 2,011,224 + ygfX BNILDI_10015 0.80 -0.7 2,011,224 + ygfX BNILDI_10015 0.80 -1.9 2,011,225 - ygfX BNILDI_10015 0.80 -0.8 2,011,323 + +0.1 2,011,324 - +0.2 2,011,353 + -0.1 2,011,353 + +1.1 2,011,399 + fldB BNILDI_10020 0.10 -0.5 2,011,400 - fldB BNILDI_10020 0.10 -0.9 2,011,427 - fldB BNILDI_10020 0.16 -0.7 2,011,731 + fldB BNILDI_10020 0.74 -0.7 2,011,732 - fldB BNILDI_10020 0.74 -2.1 2,011,742 + fldB BNILDI_10020 0.76 -0.5 2,011,772 + fldB BNILDI_10020 0.82 -0.6 2,011,772 + fldB BNILDI_10020 0.82 -1.5 2,011,772 + fldB BNILDI_10020 0.82 +0.1 2,011,772 + fldB BNILDI_10020 0.82 -0.1 2,011,772 + fldB BNILDI_10020 0.82 -0.7 2,011,773 - fldB BNILDI_10020 0.82 -1.9 2,011,796 - fldB BNILDI_10020 0.86 -0.4 2,011,831 - -0.6 2,011,861 + -0.3 2,011,905 + +1.1 2,011,906 - -1.5 2,011,910 + -0.3 2,011,924 + +0.2 2,011,946 - +0.6 2,011,967 + +0.2 2,012,050 + +1.4 2,012,405 + xerD BNILDI_10025 0.47 -0.2 2,012,405 + xerD BNILDI_10025 0.47 -0.3 2,012,406 - xerD BNILDI_10025 0.48 +0.7 2,012,406 - xerD BNILDI_10025 0.48 -1.2 2,012,406 - xerD BNILDI_10025 0.48 -1.1 2,012,739 + xerD BNILDI_10025 0.85 -2.8 2,012,914 + +0.6 2,012,914 + +1.1 2,012,915 - -0.6 2,012,915 - -0.1 2,013,071 + dsbC BNILDI_10030 0.24 -0.3 2,013,072 - dsbC BNILDI_10030 0.24 +0.8 2,013,072 - dsbC BNILDI_10030 0.24 +0.5 2,013,094 + dsbC BNILDI_10030 0.27 -0.6 2,013,095 - dsbC BNILDI_10030 0.27 +0.1 2,013,095 - dsbC BNILDI_10030 0.27 -0.1 2,013,113 + dsbC BNILDI_10030 0.30 +0.1 2,013,113 + dsbC BNILDI_10030 0.30 -0.2 2,013,114 - dsbC BNILDI_10030 0.30 +0.0 2,013,244 + dsbC BNILDI_10030 0.48 -0.2 2,013,245 - dsbC BNILDI_10030 0.49 -0.3 2,013,247 + dsbC BNILDI_10030 0.49 -0.4 2,013,248 - dsbC BNILDI_10030 0.49 -0.1 2,013,292 + dsbC BNILDI_10030 0.55 +0.2 2,013,373 + dsbC BNILDI_10030 0.67 -0.2 2,013,424 + dsbC BNILDI_10030 0.74 +0.5 2,013,425 - dsbC BNILDI_10030 0.74 +0.3 2,013,457 + dsbC BNILDI_10030 0.78 +1.0 2,013,630 - -0.3 2,013,757 - +0.1 2,013,757 - +0.9 2,013,808 + recJ BNILDI_10035 0.11 -0.2 2,013,808 + recJ BNILDI_10035 0.11 +0.2
Or see this region's nucleotide sequence