Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serA and BNILDI_09915 are separated by 228 nucleotides BNILDI_09915 and BNILDI_09925 are separated by 332 nucleotides BNILDI_09925 and ygfA are separated by 100 nucleotides ygfA and zapA are separated by 245 nucleotides zapA and ygfB are separated by 167 nucleotides
BNILDI_09905: serA - phosphoglycerate dehydrogenase, at 1,993,045 to 1,994,277
serA
BNILDI_09915: BNILDI_09915 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,506 to 1,994,565
_09915
BNILDI_09925: BNILDI_09925 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,898 to 1,994,957
_09925
BNILDI_09930: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 1,995,058 to 1,995,660
ygfA
BNILDI_09940: zapA - cell division protein ZapA, at 1,995,906 to 1,996,235
zapA
BNILDI_09945: ygfB - UPF0149 family protein YgfB, at 1,996,403 to 1,996,981
ygfB
Position (kb)
1995
1996 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1994.096 kb on + strand, within serA at 1994.149 kb on + strand, within serA at 1994.150 kb on - strand, within serA at 1994.153 kb on + strand, within serA at 1994.276 kb on - strand at 1994.365 kb on - strand at 1994.506 kb on - strand at 1994.561 kb on - strand at 1994.563 kb on + strand at 1994.564 kb on - strand at 1994.564 kb on - strand at 1994.617 kb on - strand at 1994.718 kb on - strand at 1994.718 kb on - strand at 1994.718 kb on - strand at 1994.897 kb on + strand at 1994.926 kb on + strand, within BNILDI_09925 at 1994.956 kb on - strand at 1994.996 kb on + strand at 1995.003 kb on + strand at 1995.008 kb on + strand at 1995.176 kb on + strand, within ygfA at 1995.207 kb on - strand, within ygfA at 1995.239 kb on + strand, within ygfA at 1995.278 kb on - strand, within ygfA at 1995.366 kb on - strand, within ygfA at 1995.393 kb on + strand, within ygfA at 1995.416 kb on + strand, within ygfA at 1995.418 kb on + strand, within ygfA at 1995.418 kb on + strand, within ygfA at 1995.419 kb on - strand, within ygfA at 1995.448 kb on + strand, within ygfA at 1995.448 kb on + strand, within ygfA at 1995.449 kb on - strand, within ygfA at 1995.449 kb on - strand, within ygfA at 1995.449 kb on - strand, within ygfA at 1995.491 kb on - strand, within ygfA at 1995.649 kb on + strand at 1995.649 kb on + strand at 1995.650 kb on - strand at 1995.674 kb on + strand at 1995.884 kb on + strand at 1995.885 kb on - strand at 1995.954 kb on + strand, within zapA at 1995.954 kb on + strand, within zapA at 1995.966 kb on + strand, within zapA at 1995.966 kb on + strand, within zapA at 1995.967 kb on - strand, within zapA at 1996.001 kb on + strand, within zapA at 1996.002 kb on - strand, within zapA at 1996.048 kb on + strand, within zapA at 1996.088 kb on + strand, within zapA at 1996.101 kb on - strand, within zapA at 1996.303 kb on + strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.304 kb on - strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.305 kb on + strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.306 kb on - strand at 1996.310 kb on - strand at 1996.363 kb on - strand at 1996.367 kb on + strand at 1996.367 kb on + strand at 1996.367 kb on + strand at 1996.368 kb on - strand at 1996.368 kb on - strand at 1996.368 kb on - strand at 1996.402 kb on + strand at 1996.403 kb on - strand at 1996.410 kb on + strand at 1996.512 kb on + strand, within ygfB at 1996.512 kb on + strand, within ygfB at 1996.513 kb on - strand, within ygfB at 1996.549 kb on - strand, within ygfB at 1996.609 kb on + strand, within ygfB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,994,096 + serA BNILDI_09905 0.85 -4.6 1,994,149 + serA BNILDI_09905 0.90 -3.3 1,994,150 - serA BNILDI_09905 0.90 -5.0 1,994,153 + serA BNILDI_09905 0.90 -4.6 1,994,276 - -1.5 1,994,365 - -1.2 1,994,506 - +0.5 1,994,561 - -1.8 1,994,563 + +0.1 1,994,564 - -0.7 1,994,564 - -0.8 1,994,617 - -0.8 1,994,718 - +0.8 1,994,718 - -0.3 1,994,718 - +0.0 1,994,897 + +0.5 1,994,926 + BNILDI_09925 0.47 -0.0 1,994,956 - +0.9 1,994,996 + +0.8 1,995,003 + -0.7 1,995,008 + +0.4 1,995,176 + ygfA BNILDI_09930 0.20 -0.1 1,995,207 - ygfA BNILDI_09930 0.25 -0.2 1,995,239 + ygfA BNILDI_09930 0.30 +0.3 1,995,278 - ygfA BNILDI_09930 0.36 +1.4 1,995,366 - ygfA BNILDI_09930 0.51 -0.1 1,995,393 + ygfA BNILDI_09930 0.56 +0.2 1,995,416 + ygfA BNILDI_09930 0.59 +0.6 1,995,418 + ygfA BNILDI_09930 0.60 +1.0 1,995,418 + ygfA BNILDI_09930 0.60 +0.6 1,995,419 - ygfA BNILDI_09930 0.60 -0.2 1,995,448 + ygfA BNILDI_09930 0.65 +0.3 1,995,448 + ygfA BNILDI_09930 0.65 +0.3 1,995,449 - ygfA BNILDI_09930 0.65 +0.5 1,995,449 - ygfA BNILDI_09930 0.65 -0.9 1,995,449 - ygfA BNILDI_09930 0.65 -0.4 1,995,491 - ygfA BNILDI_09930 0.72 +0.1 1,995,649 + +0.1 1,995,649 + -0.1 1,995,650 - -0.2 1,995,674 + -0.6 1,995,884 + -3.2 1,995,885 - +0.3 1,995,954 + zapA BNILDI_09940 0.15 +0.5 1,995,954 + zapA BNILDI_09940 0.15 -1.1 1,995,966 + zapA BNILDI_09940 0.18 -0.1 1,995,966 + zapA BNILDI_09940 0.18 +0.1 1,995,967 - zapA BNILDI_09940 0.18 -1.0 1,996,001 + zapA BNILDI_09940 0.29 -0.7 1,996,002 - zapA BNILDI_09940 0.29 +0.7 1,996,048 + zapA BNILDI_09940 0.43 -0.3 1,996,088 + zapA BNILDI_09940 0.55 -0.3 1,996,101 - zapA BNILDI_09940 0.59 -1.0 1,996,303 + +0.3 1,996,304 - -0.3 1,996,304 - +0.4 1,996,304 - -1.1 1,996,304 - +0.4 1,996,305 + -0.0 1,996,305 + +0.1 1,996,305 + -1.3 1,996,305 + +0.8 1,996,305 + -0.1 1,996,305 + -0.3 1,996,305 + -0.0 1,996,305 + +0.0 1,996,305 + +0.2 1,996,306 - -0.0 1,996,306 - +1.5 1,996,306 - -0.4 1,996,306 - -1.1 1,996,306 - -0.2 1,996,310 - +0.3 1,996,363 - -1.3 1,996,367 + -0.2 1,996,367 + -0.0 1,996,367 + +0.1 1,996,368 - -0.1 1,996,368 - -0.5 1,996,368 - -0.5 1,996,402 + +0.1 1,996,403 - +0.7 1,996,410 + -1.7 1,996,512 + ygfB BNILDI_09945 0.19 +0.0 1,996,512 + ygfB BNILDI_09945 0.19 -1.8 1,996,513 - ygfB BNILDI_09945 0.19 +0.3 1,996,549 - ygfB BNILDI_09945 0.25 -0.9 1,996,609 + ygfB BNILDI_09945 0.36 -1.6
Or see this region's nucleotide sequence