Strain Fitness in Escherichia coli ECRC62 around BNILDI_09930

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntserA and BNILDI_09915 are separated by 228 nucleotidesBNILDI_09915 and BNILDI_09925 are separated by 332 nucleotidesBNILDI_09925 and ygfA are separated by 100 nucleotidesygfA and zapA are separated by 245 nucleotideszapA and ygfB are separated by 167 nucleotides BNILDI_09905: serA - phosphoglycerate dehydrogenase, at 1,993,045 to 1,994,277 serA BNILDI_09915: BNILDI_09915 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,506 to 1,994,565 _09915 BNILDI_09925: BNILDI_09925 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,898 to 1,994,957 _09925 BNILDI_09930: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 1,995,058 to 1,995,660 ygfA BNILDI_09940: zapA - cell division protein ZapA, at 1,995,906 to 1,996,235 zapA BNILDI_09945: ygfB - UPF0149 family protein YgfB, at 1,996,403 to 1,996,981 ygfB Position (kb) 1995 1996Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1994.096 kb on + strand, within serAat 1994.149 kb on + strand, within serAat 1994.150 kb on - strand, within serAat 1994.153 kb on + strand, within serAat 1994.276 kb on - strandat 1994.365 kb on - strandat 1994.506 kb on - strandat 1994.561 kb on - strandat 1994.563 kb on + strandat 1994.564 kb on - strandat 1994.564 kb on - strandat 1994.617 kb on - strandat 1994.718 kb on - strandat 1994.718 kb on - strandat 1994.718 kb on - strandat 1994.897 kb on + strandat 1994.926 kb on + strand, within BNILDI_09925at 1994.956 kb on - strandat 1994.996 kb on + strandat 1995.003 kb on + strandat 1995.008 kb on + strandat 1995.176 kb on + strand, within ygfAat 1995.207 kb on - strand, within ygfAat 1995.239 kb on + strand, within ygfAat 1995.278 kb on - strand, within ygfAat 1995.366 kb on - strand, within ygfAat 1995.393 kb on + strand, within ygfAat 1995.416 kb on + strand, within ygfAat 1995.418 kb on + strand, within ygfAat 1995.418 kb on + strand, within ygfAat 1995.419 kb on - strand, within ygfAat 1995.448 kb on + strand, within ygfAat 1995.448 kb on + strand, within ygfAat 1995.449 kb on - strand, within ygfAat 1995.449 kb on - strand, within ygfAat 1995.449 kb on - strand, within ygfAat 1995.491 kb on - strand, within ygfAat 1995.649 kb on + strandat 1995.649 kb on + strandat 1995.650 kb on - strandat 1995.674 kb on + strandat 1995.884 kb on + strandat 1995.885 kb on - strandat 1995.954 kb on + strand, within zapAat 1995.954 kb on + strand, within zapAat 1995.966 kb on + strand, within zapAat 1995.966 kb on + strand, within zapAat 1995.967 kb on - strand, within zapAat 1996.001 kb on + strand, within zapAat 1996.002 kb on - strand, within zapAat 1996.048 kb on + strand, within zapAat 1996.088 kb on + strand, within zapAat 1996.101 kb on - strand, within zapAat 1996.303 kb on + strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.304 kb on - strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.305 kb on + strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.306 kb on - strandat 1996.310 kb on - strandat 1996.363 kb on - strandat 1996.367 kb on + strandat 1996.367 kb on + strandat 1996.367 kb on + strandat 1996.368 kb on - strandat 1996.368 kb on - strandat 1996.368 kb on - strandat 1996.402 kb on + strandat 1996.403 kb on - strandat 1996.410 kb on + strandat 1996.512 kb on + strand, within ygfBat 1996.512 kb on + strand, within ygfBat 1996.513 kb on - strand, within ygfBat 1996.549 kb on - strand, within ygfBat 1996.609 kb on + strand, within ygfB

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,994,096 + serA BNILDI_09905 0.85 -4.6
1,994,149 + serA BNILDI_09905 0.90 -3.3
1,994,150 - serA BNILDI_09905 0.90 -5.0
1,994,153 + serA BNILDI_09905 0.90 -4.6
1,994,276 - -1.5
1,994,365 - -1.2
1,994,506 - +0.5
1,994,561 - -1.8
1,994,563 + +0.1
1,994,564 - -0.7
1,994,564 - -0.8
1,994,617 - -0.8
1,994,718 - +0.8
1,994,718 - -0.3
1,994,718 - +0.0
1,994,897 + +0.5
1,994,926 + BNILDI_09925 0.47 -0.0
1,994,956 - +0.9
1,994,996 + +0.8
1,995,003 + -0.7
1,995,008 + +0.4
1,995,176 + ygfA BNILDI_09930 0.20 -0.1
1,995,207 - ygfA BNILDI_09930 0.25 -0.2
1,995,239 + ygfA BNILDI_09930 0.30 +0.3
1,995,278 - ygfA BNILDI_09930 0.36 +1.4
1,995,366 - ygfA BNILDI_09930 0.51 -0.1
1,995,393 + ygfA BNILDI_09930 0.56 +0.2
1,995,416 + ygfA BNILDI_09930 0.59 +0.6
1,995,418 + ygfA BNILDI_09930 0.60 +1.0
1,995,418 + ygfA BNILDI_09930 0.60 +0.6
1,995,419 - ygfA BNILDI_09930 0.60 -0.2
1,995,448 + ygfA BNILDI_09930 0.65 +0.3
1,995,448 + ygfA BNILDI_09930 0.65 +0.3
1,995,449 - ygfA BNILDI_09930 0.65 +0.5
1,995,449 - ygfA BNILDI_09930 0.65 -0.9
1,995,449 - ygfA BNILDI_09930 0.65 -0.4
1,995,491 - ygfA BNILDI_09930 0.72 +0.1
1,995,649 + +0.1
1,995,649 + -0.1
1,995,650 - -0.2
1,995,674 + -0.6
1,995,884 + -3.2
1,995,885 - +0.3
1,995,954 + zapA BNILDI_09940 0.15 +0.5
1,995,954 + zapA BNILDI_09940 0.15 -1.1
1,995,966 + zapA BNILDI_09940 0.18 -0.1
1,995,966 + zapA BNILDI_09940 0.18 +0.1
1,995,967 - zapA BNILDI_09940 0.18 -1.0
1,996,001 + zapA BNILDI_09940 0.29 -0.7
1,996,002 - zapA BNILDI_09940 0.29 +0.7
1,996,048 + zapA BNILDI_09940 0.43 -0.3
1,996,088 + zapA BNILDI_09940 0.55 -0.3
1,996,101 - zapA BNILDI_09940 0.59 -1.0
1,996,303 + +0.3
1,996,304 - -0.3
1,996,304 - +0.4
1,996,304 - -1.1
1,996,304 - +0.4
1,996,305 + -0.0
1,996,305 + +0.1
1,996,305 + -1.3
1,996,305 + +0.8
1,996,305 + -0.1
1,996,305 + -0.3
1,996,305 + -0.0
1,996,305 + +0.0
1,996,305 + +0.2
1,996,306 - -0.0
1,996,306 - +1.5
1,996,306 - -0.4
1,996,306 - -1.1
1,996,306 - -0.2
1,996,310 - +0.3
1,996,363 - -1.3
1,996,367 + -0.2
1,996,367 + -0.0
1,996,367 + +0.1
1,996,368 - -0.1
1,996,368 - -0.5
1,996,368 - -0.5
1,996,402 + +0.1
1,996,403 - +0.7
1,996,410 + -1.7
1,996,512 + ygfB BNILDI_09945 0.19 +0.0
1,996,512 + ygfB BNILDI_09945 0.19 -1.8
1,996,513 - ygfB BNILDI_09945 0.19 +0.3
1,996,549 - ygfB BNILDI_09945 0.25 -0.9
1,996,609 + ygfB BNILDI_09945 0.36 -1.6

Or see this region's nucleotide sequence