Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqfE and rpiA are separated by 55 nucleotides rpiA and serA are separated by 255 nucleotides serA and BNILDI_09915 are separated by 228 nucleotides BNILDI_09915 and BNILDI_09925 are separated by 332 nucleotides BNILDI_09925 and ygfA are separated by 100 nucleotides
BNILDI_09895: yqfE - Putative protein YqfE, at 1,991,844 to 1,992,074
yqfE
BNILDI_09900: rpiA - ribose-5-phosphate isomerase RpiA, at 1,992,130 to 1,992,789
rpiA
BNILDI_09905: serA - phosphoglycerate dehydrogenase, at 1,993,045 to 1,994,277
serA
BNILDI_09915: BNILDI_09915 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,506 to 1,994,565
_09915
BNILDI_09925: BNILDI_09925 - Type I toxin-antitoxin system Ibs family toxin, at 1,994,898 to 1,994,957
_09925
BNILDI_09930: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 1,995,058 to 1,995,660
ygfA
Position (kb) 1993
1994
1995 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1992.088 kb on + strand at 1992.091 kb on - strand at 1992.092 kb on + strand at 1992.092 kb on + strand at 1992.092 kb on + strand at 1992.974 kb on + strand at 1992.974 kb on + strand at 1992.987 kb on + strand at 1993.056 kb on - strand at 1993.077 kb on + strand at 1993.295 kb on + strand, within serA at 1993.350 kb on + strand, within serA at 1993.351 kb on - strand, within serA at 1993.368 kb on + strand, within serA at 1993.413 kb on - strand, within serA at 1993.570 kb on + strand, within serA at 1993.570 kb on + strand, within serA at 1993.571 kb on - strand, within serA at 1993.571 kb on - strand, within serA at 1993.571 kb on - strand, within serA at 1993.571 kb on - strand, within serA at 1993.625 kb on + strand, within serA at 1993.650 kb on + strand, within serA at 1993.651 kb on - strand, within serA at 1993.651 kb on - strand, within serA at 1993.839 kb on - strand, within serA at 1993.876 kb on - strand, within serA at 1993.876 kb on - strand, within serA at 1994.096 kb on + strand, within serA at 1994.149 kb on + strand, within serA at 1994.150 kb on - strand, within serA at 1994.153 kb on + strand, within serA at 1994.276 kb on - strand at 1994.365 kb on - strand at 1994.506 kb on - strand at 1994.561 kb on - strand at 1994.563 kb on + strand at 1994.564 kb on - strand at 1994.564 kb on - strand at 1994.617 kb on - strand at 1994.718 kb on - strand at 1994.718 kb on - strand at 1994.718 kb on - strand at 1994.897 kb on + strand at 1994.926 kb on + strand, within BNILDI_09925 at 1994.956 kb on - strand at 1994.996 kb on + strand at 1995.003 kb on + strand at 1995.008 kb on + strand at 1995.176 kb on + strand, within ygfA at 1995.207 kb on - strand, within ygfA at 1995.239 kb on + strand, within ygfA
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,992,088 + +0.5 1,992,091 - -0.6 1,992,092 + +0.2 1,992,092 + -1.0 1,992,092 + +0.1 1,992,974 + -5.5 1,992,974 + -4.7 1,992,987 + -0.4 1,993,056 - -4.3 1,993,077 + -2.5 1,993,295 + serA BNILDI_09905 0.20 -3.7 1,993,350 + serA BNILDI_09905 0.25 -0.6 1,993,351 - serA BNILDI_09905 0.25 -5.0 1,993,368 + serA BNILDI_09905 0.26 -2.9 1,993,413 - serA BNILDI_09905 0.30 -4.7 1,993,570 + serA BNILDI_09905 0.43 -5.1 1,993,570 + serA BNILDI_09905 0.43 -1.8 1,993,571 - serA BNILDI_09905 0.43 -4.4 1,993,571 - serA BNILDI_09905 0.43 -1.9 1,993,571 - serA BNILDI_09905 0.43 -0.2 1,993,571 - serA BNILDI_09905 0.43 -4.0 1,993,625 + serA BNILDI_09905 0.47 -3.1 1,993,650 + serA BNILDI_09905 0.49 -1.7 1,993,651 - serA BNILDI_09905 0.49 -2.0 1,993,651 - serA BNILDI_09905 0.49 -2.7 1,993,839 - serA BNILDI_09905 0.64 -2.7 1,993,876 - serA BNILDI_09905 0.67 -2.1 1,993,876 - serA BNILDI_09905 0.67 -3.5 1,994,096 + serA BNILDI_09905 0.85 -4.6 1,994,149 + serA BNILDI_09905 0.90 -3.3 1,994,150 - serA BNILDI_09905 0.90 -5.0 1,994,153 + serA BNILDI_09905 0.90 -4.6 1,994,276 - -1.5 1,994,365 - -1.2 1,994,506 - +0.5 1,994,561 - -1.8 1,994,563 + +0.1 1,994,564 - -0.7 1,994,564 - -0.8 1,994,617 - -0.8 1,994,718 - +0.8 1,994,718 - -0.3 1,994,718 - +0.0 1,994,897 + +0.5 1,994,926 + BNILDI_09925 0.47 -0.0 1,994,956 - +0.9 1,994,996 + +0.8 1,995,003 + -0.7 1,995,008 + +0.4 1,995,176 + ygfA BNILDI_09930 0.20 -0.1 1,995,207 - ygfA BNILDI_09930 0.25 -0.2 1,995,239 + ygfA BNILDI_09930 0.30 +0.3
Or see this region's nucleotide sequence