Strain Fitness in Escherichia coli ECRC62 around BNILDI_09365

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnhA and dnaQ are separated by 55 nucleotidesdnaQ and Asp_trna are separated by 132 nucleotidesAsp_trna and yafT are separated by 330 nucleotides BNILDI_09355: rnhA - ribonuclease HI, at 1,874,761 to 1,875,228 rnhA BNILDI_09360: dnaQ - DNA polymerase III subunit epsilon, at 1,875,284 to 1,876,024 dnaQ BNILDI_09365: Asp_trna - tRNA-Asp(gtc), at 1,876,157 to 1,876,233 _trna BNILDI_09370: yafT - Uncharacterized lipoprotein YafT, at 1,876,564 to 1,877,349 yafT Position (kb) 1876 1877Strain fitness (log2 ratio) -1 0 1at 1875.157 kb on - strand, within rnhAat 1875.262 kb on + strandat 1876.023 kb on - strandat 1876.033 kb on - strandat 1876.465 kb on + strandat 1877.072 kb on - strand, within yafTat 1877.097 kb on - strand, within yafTat 1877.212 kb on + strand, within yafT

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,875,157 - rnhA BNILDI_09355 0.85 +0.3
1,875,262 + +0.1
1,876,023 - -0.1
1,876,033 - +0.2
1,876,465 + +0.6
1,877,072 - yafT BNILDI_09370 0.65 -0.2
1,877,097 - yafT BNILDI_09370 0.68 +1.6
1,877,212 + yafT BNILDI_09370 0.82 -0.2

Or see this region's nucleotide sequence