Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gloB and yafS are separated by 33 nucleotides yafS and rnhA overlap by 4 nucleotides rnhA and dnaQ are separated by 55 nucleotides dnaQ and Asp_trna are separated by 132 nucleotides
BNILDI_09345: gloB - hydroxyacylglutathione hydrolase, at 1,873,253 to 1,874,008
gloB
BNILDI_09350: yafS - Uncharacterized protein YafS, at 1,874,042 to 1,874,764
yafS
BNILDI_09355: rnhA - ribonuclease HI, at 1,874,761 to 1,875,228
rnhA
BNILDI_09360: dnaQ - DNA polymerase III subunit epsilon, at 1,875,284 to 1,876,024
dnaQ
BNILDI_09365: Asp_trna - tRNA-Asp(gtc), at 1,876,157 to 1,876,233
_trna
Position (kb) 1874
1875
1876 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1873.785 kb on + strand, within gloB at 1873.785 kb on + strand, within gloB at 1873.785 kb on + strand, within gloB at 1873.786 kb on - strand, within gloB at 1873.786 kb on - strand, within gloB at 1873.824 kb on - strand, within gloB at 1873.863 kb on - strand, within gloB at 1873.971 kb on + strand at 1873.971 kb on + strand at 1873.971 kb on + strand at 1873.971 kb on + strand at 1874.009 kb on - strand at 1874.014 kb on - strand at 1874.021 kb on - strand at 1874.021 kb on - strand at 1874.336 kb on - strand, within yafS at 1874.336 kb on - strand, within yafS at 1874.434 kb on + strand, within yafS at 1874.434 kb on + strand, within yafS at 1874.435 kb on - strand, within yafS at 1874.439 kb on + strand, within yafS at 1874.467 kb on - strand, within yafS at 1874.486 kb on + strand, within yafS at 1874.489 kb on - strand, within yafS at 1874.617 kb on + strand, within yafS at 1874.617 kb on + strand, within yafS at 1874.618 kb on - strand, within yafS at 1874.637 kb on + strand, within yafS at 1874.638 kb on - strand, within yafS at 1874.638 kb on - strand, within yafS at 1874.644 kb on + strand, within yafS at 1874.644 kb on + strand, within yafS at 1874.750 kb on - strand at 1874.784 kb on + strand at 1874.785 kb on - strand at 1874.785 kb on - strand at 1874.980 kb on - strand, within rnhA at 1875.011 kb on + strand, within rnhA at 1875.151 kb on + strand, within rnhA at 1875.151 kb on + strand, within rnhA at 1875.157 kb on - strand, within rnhA at 1875.262 kb on + strand at 1876.023 kb on - strand at 1876.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,873,785 + gloB BNILDI_09345 0.70 -0.4 1,873,785 + gloB BNILDI_09345 0.70 +0.8 1,873,785 + gloB BNILDI_09345 0.70 +0.1 1,873,786 - gloB BNILDI_09345 0.71 -0.6 1,873,786 - gloB BNILDI_09345 0.71 -0.3 1,873,824 - gloB BNILDI_09345 0.76 -0.9 1,873,863 - gloB BNILDI_09345 0.81 -1.2 1,873,971 + -0.6 1,873,971 + -0.1 1,873,971 + -0.3 1,873,971 + -0.7 1,874,009 - +0.4 1,874,014 - +1.6 1,874,021 - -0.3 1,874,021 - -0.3 1,874,336 - yafS BNILDI_09350 0.41 +2.7 1,874,336 - yafS BNILDI_09350 0.41 +0.3 1,874,434 + yafS BNILDI_09350 0.54 +0.2 1,874,434 + yafS BNILDI_09350 0.54 +0.1 1,874,435 - yafS BNILDI_09350 0.54 -0.9 1,874,439 + yafS BNILDI_09350 0.55 +0.2 1,874,467 - yafS BNILDI_09350 0.59 -2.7 1,874,486 + yafS BNILDI_09350 0.61 -0.1 1,874,489 - yafS BNILDI_09350 0.62 +0.0 1,874,617 + yafS BNILDI_09350 0.80 +0.0 1,874,617 + yafS BNILDI_09350 0.80 -0.2 1,874,618 - yafS BNILDI_09350 0.80 +0.1 1,874,637 + yafS BNILDI_09350 0.82 +0.2 1,874,638 - yafS BNILDI_09350 0.82 +0.4 1,874,638 - yafS BNILDI_09350 0.82 +0.0 1,874,644 + yafS BNILDI_09350 0.83 -1.3 1,874,644 + yafS BNILDI_09350 0.83 +0.6 1,874,750 - +1.1 1,874,784 + -0.0 1,874,785 - -0.0 1,874,785 - -0.3 1,874,980 - rnhA BNILDI_09355 0.47 -0.3 1,875,011 + rnhA BNILDI_09355 0.53 +0.6 1,875,151 + rnhA BNILDI_09355 0.83 +1.2 1,875,151 + rnhA BNILDI_09355 0.83 +1.8 1,875,157 - rnhA BNILDI_09355 0.85 +0.3 1,875,262 + +0.1 1,876,023 - -0.1 1,876,033 - +0.2
Or see this region's nucleotide sequence