Strain Fitness in Escherichia coli ECRC62 around BNILDI_09320

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nt23S_rrna and 5S_rrna are separated by 95 nucleotides5S_rrna and Asp_trna are separated by 193 nucleotidesAsp_trna and dkgB are separated by 162 nucleotidesdkgB and yafC overlap by 4 nucleotides BNILDI_09305: 23S_rrna - 23S ribosomal RNA, at 1,864,623 to 1,867,528 _rrna BNILDI_09310: 5S_rrna - 5S ribosomal RNA, at 1,867,624 to 1,867,739 _rrna BNILDI_09315: Asp_trna - tRNA-Asp(gtc), at 1,867,933 to 1,868,009 _trna BNILDI_09320: dkgB - 2,5-didehydrogluconate reductase DkgB, at 1,868,172 to 1,868,975 dkgB BNILDI_09325: yafC - DNA-binding transcriptional regulator YafC, at 1,868,972 to 1,869,886 yafC Position (kb) 1868 1869Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1867.866 kb on + strandat 1868.065 kb on + strandat 1868.065 kb on + strandat 1868.158 kb on - strandat 1868.168 kb on + strandat 1868.169 kb on - strandat 1868.171 kb on + strandat 1868.172 kb on - strandat 1868.287 kb on - strand, within dkgBat 1868.349 kb on + strand, within dkgBat 1868.349 kb on + strand, within dkgBat 1868.439 kb on + strand, within dkgBat 1868.439 kb on + strand, within dkgBat 1868.657 kb on + strand, within dkgBat 1868.693 kb on + strand, within dkgBat 1868.696 kb on + strand, within dkgBat 1868.703 kb on + strand, within dkgBat 1868.724 kb on - strand, within dkgBat 1868.846 kb on + strand, within dkgBat 1868.846 kb on + strand, within dkgBat 1868.846 kb on + strand, within dkgBat 1868.846 kb on + strand, within dkgBat 1868.846 kb on + strand, within dkgBat 1868.900 kb on + strandat 1869.008 kb on + strandat 1869.031 kb on + strandat 1869.069 kb on + strand, within yafCat 1869.072 kb on + strand, within yafCat 1869.120 kb on - strand, within yafCat 1869.162 kb on + strand, within yafCat 1869.163 kb on - strand, within yafCat 1869.241 kb on - strand, within yafCat 1869.241 kb on - strand, within yafCat 1869.254 kb on + strand, within yafCat 1869.254 kb on + strand, within yafCat 1869.255 kb on - strand, within yafCat 1869.255 kb on - strand, within yafCat 1869.255 kb on - strand, within yafCat 1869.322 kb on + strand, within yafCat 1869.322 kb on + strand, within yafCat 1869.323 kb on - strand, within yafCat 1869.332 kb on + strand, within yafCat 1869.333 kb on - strand, within yafCat 1869.372 kb on + strand, within yafCat 1869.425 kb on + strand, within yafCat 1869.446 kb on - strand, within yafCat 1869.510 kb on - strand, within yafCat 1869.677 kb on + strand, within yafCat 1869.677 kb on + strand, within yafCat 1869.690 kb on + strand, within yafCat 1869.807 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,867,866 + +0.0
1,868,065 + -0.7
1,868,065 + -0.8
1,868,158 - +0.8
1,868,168 + -0.0
1,868,169 - +0.5
1,868,171 + +0.5
1,868,172 - -1.2
1,868,287 - dkgB BNILDI_09320 0.14 -0.5
1,868,349 + dkgB BNILDI_09320 0.22 -1.3
1,868,349 + dkgB BNILDI_09320 0.22 +0.7
1,868,439 + dkgB BNILDI_09320 0.33 -1.8
1,868,439 + dkgB BNILDI_09320 0.33 -0.2
1,868,657 + dkgB BNILDI_09320 0.60 +0.2
1,868,693 + dkgB BNILDI_09320 0.65 -0.8
1,868,696 + dkgB BNILDI_09320 0.65 +0.6
1,868,703 + dkgB BNILDI_09320 0.66 +1.7
1,868,724 - dkgB BNILDI_09320 0.69 -0.2
1,868,846 + dkgB BNILDI_09320 0.84 +1.1
1,868,846 + dkgB BNILDI_09320 0.84 -1.0
1,868,846 + dkgB BNILDI_09320 0.84 +0.2
1,868,846 + dkgB BNILDI_09320 0.84 -0.3
1,868,846 + dkgB BNILDI_09320 0.84 -3.2
1,868,900 + -0.3
1,869,008 + +0.4
1,869,031 + -0.0
1,869,069 + yafC BNILDI_09325 0.11 -0.1
1,869,072 + yafC BNILDI_09325 0.11 +0.4
1,869,120 - yafC BNILDI_09325 0.16 -0.5
1,869,162 + yafC BNILDI_09325 0.21 -0.9
1,869,163 - yafC BNILDI_09325 0.21 -0.7
1,869,241 - yafC BNILDI_09325 0.29 +0.4
1,869,241 - yafC BNILDI_09325 0.29 +0.1
1,869,254 + yafC BNILDI_09325 0.31 +0.7
1,869,254 + yafC BNILDI_09325 0.31 +0.7
1,869,255 - yafC BNILDI_09325 0.31 +0.0
1,869,255 - yafC BNILDI_09325 0.31 +0.1
1,869,255 - yafC BNILDI_09325 0.31 +0.3
1,869,322 + yafC BNILDI_09325 0.38 -1.3
1,869,322 + yafC BNILDI_09325 0.38 -0.0
1,869,323 - yafC BNILDI_09325 0.38 +2.4
1,869,332 + yafC BNILDI_09325 0.39 -0.7
1,869,333 - yafC BNILDI_09325 0.39 +0.5
1,869,372 + yafC BNILDI_09325 0.44 -1.1
1,869,425 + yafC BNILDI_09325 0.50 -0.4
1,869,446 - yafC BNILDI_09325 0.52 +0.6
1,869,510 - yafC BNILDI_09325 0.59 -0.8
1,869,677 + yafC BNILDI_09325 0.77 -0.3
1,869,677 + yafC BNILDI_09325 0.77 -0.5
1,869,690 + yafC BNILDI_09325 0.78 -0.8
1,869,807 - -0.1

Or see this region's nucleotide sequence