Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gluQRS and dksA are separated by 36 nucleotides dksA and sfsA are separated by 177 nucleotides sfsA and thpR are separated by 14 nucleotides
BNILDI_08975: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 1,797,659 to 1,798,585
gluQRS
BNILDI_08980: dksA - RNA polymerase-binding protein DksA, at 1,798,622 to 1,799,077
dksA
BNILDI_08985: sfsA - DNA/RNA nuclease SfsA, at 1,799,255 to 1,799,959
sfsA
BNILDI_08990: thpR - RNA 2',3'-cyclic phosphodiesterase, at 1,799,974 to 1,800,504
thpR
Position (kb)
1798
1799
1800 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 1797.655 kb on + strand at 1797.912 kb on + strand, within gluQRS at 1798.073 kb on - strand, within gluQRS at 1798.108 kb on + strand, within gluQRS at 1798.246 kb on + strand, within gluQRS at 1798.258 kb on - strand, within gluQRS at 1798.261 kb on - strand, within gluQRS at 1798.265 kb on + strand, within gluQRS at 1798.265 kb on + strand, within gluQRS at 1798.266 kb on - strand, within gluQRS at 1798.266 kb on - strand, within gluQRS at 1798.424 kb on + strand, within gluQRS at 1798.467 kb on + strand, within gluQRS at 1798.468 kb on - strand, within gluQRS at 1798.538 kb on - strand at 1798.539 kb on + strand at 1798.539 kb on + strand at 1798.539 kb on + strand at 1798.539 kb on + strand at 1798.539 kb on + strand at 1798.539 kb on + strand at 1798.540 kb on - strand at 1798.540 kb on - strand at 1798.540 kb on - strand at 1798.540 kb on - strand at 1798.540 kb on - strand at 1798.600 kb on - strand at 1798.620 kb on - strand at 1798.620 kb on - strand at 1798.798 kb on + strand, within dksA at 1798.894 kb on + strand, within dksA at 1798.894 kb on + strand, within dksA at 1798.895 kb on - strand, within dksA at 1798.895 kb on - strand, within dksA at 1798.895 kb on - strand, within dksA at 1798.895 kb on - strand, within dksA at 1798.901 kb on - strand, within dksA at 1798.934 kb on - strand, within dksA at 1798.986 kb on + strand, within dksA at 1798.987 kb on - strand, within dksA at 1799.139 kb on - strand at 1799.143 kb on - strand at 1799.143 kb on - strand at 1799.236 kb on + strand at 1799.245 kb on - strand at 1799.248 kb on + strand at 1799.249 kb on - strand at 1799.253 kb on + strand at 1799.253 kb on + strand at 1799.256 kb on + strand at 1799.317 kb on - strand at 1799.367 kb on + strand, within sfsA at 1799.517 kb on + strand, within sfsA at 1799.561 kb on - strand, within sfsA at 1799.561 kb on - strand, within sfsA at 1799.562 kb on + strand, within sfsA at 1799.594 kb on - strand, within sfsA at 1799.594 kb on - strand, within sfsA at 1799.622 kb on + strand, within sfsA at 1799.647 kb on + strand, within sfsA at 1799.647 kb on + strand, within sfsA at 1799.649 kb on + strand, within sfsA at 1799.772 kb on + strand, within sfsA at 1799.773 kb on - strand, within sfsA at 1799.862 kb on - strand, within sfsA at 1799.862 kb on - strand, within sfsA at 1799.913 kb on + strand at 1799.925 kb on - strand at 1799.940 kb on - strand at 1800.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,797,655 + -0.7 1,797,912 + gluQRS BNILDI_08975 0.27 +1.3 1,798,073 - gluQRS BNILDI_08975 0.45 -0.4 1,798,108 + gluQRS BNILDI_08975 0.48 -0.2 1,798,246 + gluQRS BNILDI_08975 0.63 +0.5 1,798,258 - gluQRS BNILDI_08975 0.65 +0.3 1,798,261 - gluQRS BNILDI_08975 0.65 +0.1 1,798,265 + gluQRS BNILDI_08975 0.65 -0.8 1,798,265 + gluQRS BNILDI_08975 0.65 +1.6 1,798,266 - gluQRS BNILDI_08975 0.65 -2.1 1,798,266 - gluQRS BNILDI_08975 0.65 +1.8 1,798,424 + gluQRS BNILDI_08975 0.83 -0.0 1,798,467 + gluQRS BNILDI_08975 0.87 +1.6 1,798,468 - gluQRS BNILDI_08975 0.87 -2.0 1,798,538 - -0.1 1,798,539 + +0.2 1,798,539 + +0.1 1,798,539 + +0.3 1,798,539 + +0.0 1,798,539 + +0.1 1,798,539 + -0.5 1,798,540 - -0.9 1,798,540 - +0.1 1,798,540 - -0.2 1,798,540 - -0.6 1,798,540 - +0.5 1,798,600 - -0.1 1,798,620 - -1.4 1,798,620 - -0.4 1,798,798 + dksA BNILDI_08980 0.39 -3.0 1,798,894 + dksA BNILDI_08980 0.60 -3.3 1,798,894 + dksA BNILDI_08980 0.60 -5.2 1,798,895 - dksA BNILDI_08980 0.60 -6.1 1,798,895 - dksA BNILDI_08980 0.60 -4.0 1,798,895 - dksA BNILDI_08980 0.60 -0.3 1,798,895 - dksA BNILDI_08980 0.60 -3.9 1,798,901 - dksA BNILDI_08980 0.61 -4.4 1,798,934 - dksA BNILDI_08980 0.68 -2.9 1,798,986 + dksA BNILDI_08980 0.80 -5.7 1,798,987 - dksA BNILDI_08980 0.80 -3.1 1,799,139 - -0.8 1,799,143 - -0.5 1,799,143 - -0.7 1,799,236 + -0.9 1,799,245 - +1.0 1,799,248 + +1.0 1,799,249 - +4.3 1,799,253 + -0.5 1,799,253 + +0.6 1,799,256 + -0.5 1,799,317 - -1.4 1,799,367 + sfsA BNILDI_08985 0.16 +0.7 1,799,517 + sfsA BNILDI_08985 0.37 -1.7 1,799,561 - sfsA BNILDI_08985 0.43 +0.9 1,799,561 - sfsA BNILDI_08985 0.43 -0.7 1,799,562 + sfsA BNILDI_08985 0.44 -0.3 1,799,594 - sfsA BNILDI_08985 0.48 -0.1 1,799,594 - sfsA BNILDI_08985 0.48 +0.4 1,799,622 + sfsA BNILDI_08985 0.52 -0.2 1,799,647 + sfsA BNILDI_08985 0.56 -0.8 1,799,647 + sfsA BNILDI_08985 0.56 +0.6 1,799,649 + sfsA BNILDI_08985 0.56 -1.9 1,799,772 + sfsA BNILDI_08985 0.73 -0.2 1,799,773 - sfsA BNILDI_08985 0.73 +0.4 1,799,862 - sfsA BNILDI_08985 0.86 -0.5 1,799,862 - sfsA BNILDI_08985 0.86 -1.7 1,799,913 + -0.8 1,799,925 - +0.0 1,799,940 - -1.9 1,800,009 - +1.5
Or see this region's nucleotide sequence