Strain Fitness in Escherichia coli ECRC62 around BNILDI_08980

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgluQRS and dksA are separated by 36 nucleotidesdksA and sfsA are separated by 177 nucleotidessfsA and thpR are separated by 14 nucleotides BNILDI_08975: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 1,797,659 to 1,798,585 gluQRS BNILDI_08980: dksA - RNA polymerase-binding protein DksA, at 1,798,622 to 1,799,077 dksA BNILDI_08985: sfsA - DNA/RNA nuclease SfsA, at 1,799,255 to 1,799,959 sfsA BNILDI_08990: thpR - RNA 2',3'-cyclic phosphodiesterase, at 1,799,974 to 1,800,504 thpR Position (kb) 1798 1799 1800Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2 3 4at 1797.655 kb on + strandat 1797.912 kb on + strand, within gluQRSat 1798.073 kb on - strand, within gluQRSat 1798.108 kb on + strand, within gluQRSat 1798.246 kb on + strand, within gluQRSat 1798.258 kb on - strand, within gluQRSat 1798.261 kb on - strand, within gluQRSat 1798.265 kb on + strand, within gluQRSat 1798.265 kb on + strand, within gluQRSat 1798.266 kb on - strand, within gluQRSat 1798.266 kb on - strand, within gluQRSat 1798.424 kb on + strand, within gluQRSat 1798.467 kb on + strand, within gluQRSat 1798.468 kb on - strand, within gluQRSat 1798.538 kb on - strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.539 kb on + strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.540 kb on - strandat 1798.600 kb on - strandat 1798.620 kb on - strandat 1798.620 kb on - strandat 1798.798 kb on + strand, within dksAat 1798.894 kb on + strand, within dksAat 1798.894 kb on + strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.895 kb on - strand, within dksAat 1798.901 kb on - strand, within dksAat 1798.934 kb on - strand, within dksAat 1798.986 kb on + strand, within dksAat 1798.987 kb on - strand, within dksAat 1799.139 kb on - strandat 1799.143 kb on - strandat 1799.143 kb on - strandat 1799.236 kb on + strandat 1799.245 kb on - strandat 1799.248 kb on + strandat 1799.249 kb on - strandat 1799.253 kb on + strandat 1799.253 kb on + strandat 1799.256 kb on + strandat 1799.317 kb on - strandat 1799.367 kb on + strand, within sfsAat 1799.517 kb on + strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.561 kb on - strand, within sfsAat 1799.562 kb on + strand, within sfsAat 1799.594 kb on - strand, within sfsAat 1799.594 kb on - strand, within sfsAat 1799.622 kb on + strand, within sfsAat 1799.647 kb on + strand, within sfsAat 1799.647 kb on + strand, within sfsAat 1799.649 kb on + strand, within sfsAat 1799.772 kb on + strand, within sfsAat 1799.773 kb on - strand, within sfsAat 1799.862 kb on - strand, within sfsAat 1799.862 kb on - strand, within sfsAat 1799.913 kb on + strandat 1799.925 kb on - strandat 1799.940 kb on - strandat 1800.009 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,797,655 + -0.7
1,797,912 + gluQRS BNILDI_08975 0.27 +1.3
1,798,073 - gluQRS BNILDI_08975 0.45 -0.4
1,798,108 + gluQRS BNILDI_08975 0.48 -0.2
1,798,246 + gluQRS BNILDI_08975 0.63 +0.5
1,798,258 - gluQRS BNILDI_08975 0.65 +0.3
1,798,261 - gluQRS BNILDI_08975 0.65 +0.1
1,798,265 + gluQRS BNILDI_08975 0.65 -0.8
1,798,265 + gluQRS BNILDI_08975 0.65 +1.6
1,798,266 - gluQRS BNILDI_08975 0.65 -2.1
1,798,266 - gluQRS BNILDI_08975 0.65 +1.8
1,798,424 + gluQRS BNILDI_08975 0.83 -0.0
1,798,467 + gluQRS BNILDI_08975 0.87 +1.6
1,798,468 - gluQRS BNILDI_08975 0.87 -2.0
1,798,538 - -0.1
1,798,539 + +0.2
1,798,539 + +0.1
1,798,539 + +0.3
1,798,539 + +0.0
1,798,539 + +0.1
1,798,539 + -0.5
1,798,540 - -0.9
1,798,540 - +0.1
1,798,540 - -0.2
1,798,540 - -0.6
1,798,540 - +0.5
1,798,600 - -0.1
1,798,620 - -1.4
1,798,620 - -0.4
1,798,798 + dksA BNILDI_08980 0.39 -3.0
1,798,894 + dksA BNILDI_08980 0.60 -3.3
1,798,894 + dksA BNILDI_08980 0.60 -5.2
1,798,895 - dksA BNILDI_08980 0.60 -6.1
1,798,895 - dksA BNILDI_08980 0.60 -4.0
1,798,895 - dksA BNILDI_08980 0.60 -0.3
1,798,895 - dksA BNILDI_08980 0.60 -3.9
1,798,901 - dksA BNILDI_08980 0.61 -4.4
1,798,934 - dksA BNILDI_08980 0.68 -2.9
1,798,986 + dksA BNILDI_08980 0.80 -5.7
1,798,987 - dksA BNILDI_08980 0.80 -3.1
1,799,139 - -0.8
1,799,143 - -0.5
1,799,143 - -0.7
1,799,236 + -0.9
1,799,245 - +1.0
1,799,248 + +1.0
1,799,249 - +4.3
1,799,253 + -0.5
1,799,253 + +0.6
1,799,256 + -0.5
1,799,317 - -1.4
1,799,367 + sfsA BNILDI_08985 0.16 +0.7
1,799,517 + sfsA BNILDI_08985 0.37 -1.7
1,799,561 - sfsA BNILDI_08985 0.43 +0.9
1,799,561 - sfsA BNILDI_08985 0.43 -0.7
1,799,562 + sfsA BNILDI_08985 0.44 -0.3
1,799,594 - sfsA BNILDI_08985 0.48 -0.1
1,799,594 - sfsA BNILDI_08985 0.48 +0.4
1,799,622 + sfsA BNILDI_08985 0.52 -0.2
1,799,647 + sfsA BNILDI_08985 0.56 -0.8
1,799,647 + sfsA BNILDI_08985 0.56 +0.6
1,799,649 + sfsA BNILDI_08985 0.56 -1.9
1,799,772 + sfsA BNILDI_08985 0.73 -0.2
1,799,773 - sfsA BNILDI_08985 0.73 +0.4
1,799,862 - sfsA BNILDI_08985 0.86 -0.5
1,799,862 - sfsA BNILDI_08985 0.86 -1.7
1,799,913 + -0.8
1,799,925 - +0.0
1,799,940 - -1.9
1,800,009 - +1.5

Or see this region's nucleotide sequence