Strain Fitness in Escherichia coli ECRC62 around BNILDI_08770

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaceE and aceF are separated by 14 nucleotidesaceF and lpdA are separated by 324 nucleotides BNILDI_08765: aceE - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 1,752,109 to 1,754,772 aceE BNILDI_08770: aceF - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,754,787 to 1,756,679 aceF BNILDI_08775: lpdA - dihydrolipoyl dehydrogenase, at 1,757,004 to 1,758,428 lpdA Position (kb) 1754 1755 1756 1757Strain fitness (log2 ratio) -3 -2 -1 0 1at 1753.897 kb on + strand, within aceEat 1753.897 kb on + strand, within aceEat 1753.898 kb on - strand, within aceEat 1753.904 kb on - strand, within aceEat 1753.904 kb on - strand, within aceEat 1753.904 kb on - strand, within aceEat 1754.139 kb on + strand, within aceEat 1754.173 kb on + strand, within aceEat 1754.174 kb on - strand, within aceEat 1754.311 kb on - strand, within aceEat 1754.651 kb on - strandat 1754.948 kb on + strandat 1754.999 kb on + strand, within aceFat 1755.877 kb on - strand, within aceFat 1756.041 kb on - strand, within aceFat 1756.370 kb on + strand, within aceFat 1756.849 kb on + strandat 1756.909 kb on + strandat 1756.913 kb on + strandat 1756.985 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,753,897 + aceE BNILDI_08765 0.67 -1.8
1,753,897 + aceE BNILDI_08765 0.67 -0.5
1,753,898 - aceE BNILDI_08765 0.67 +0.8
1,753,904 - aceE BNILDI_08765 0.67 -2.0
1,753,904 - aceE BNILDI_08765 0.67 -3.4
1,753,904 - aceE BNILDI_08765 0.67 +0.1
1,754,139 + aceE BNILDI_08765 0.76 -3.0
1,754,173 + aceE BNILDI_08765 0.77 -2.5
1,754,174 - aceE BNILDI_08765 0.78 -1.7
1,754,311 - aceE BNILDI_08765 0.83 -0.8
1,754,651 - -2.9
1,754,948 + -1.9
1,754,999 + aceF BNILDI_08770 0.11 -3.4
1,755,877 - aceF BNILDI_08770 0.58 -2.3
1,756,041 - aceF BNILDI_08770 0.66 -1.0
1,756,370 + aceF BNILDI_08770 0.84 -0.3
1,756,849 + -2.7
1,756,909 + -0.2
1,756,913 + -2.7
1,756,985 + -3.4

Or see this region's nucleotide sequence