Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvI and ilvN are separated by 2 nucleotides ilvN and fruL are separated by 32 nucleotides fruL and cra are separated by 81 nucleotides cra and mraZ are separated by 601 nucleotides
BNILDI_08565: ilvI - acetolactate synthase 3 large subunit, at 1,714,838 to 1,716,562
ilvI
BNILDI_08570: ilvN - acetolactate synthase small subunit, at 1,716,565 to 1,717,056
ilvN
BNILDI_08575: fruL - Putative fruR/shl operon leader peptide, at 1,717,089 to 1,717,154
fruL
BNILDI_08580: cra - catabolite repressor/activator, at 1,717,236 to 1,718,240
cra
BNILDI_08585: mraZ - division/cell wall cluster transcriptional repressor MraZ, at 1,718,842 to 1,719,300
mraZ
Position (kb)
1717
1718
1719 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1716.302 kb on + strand, within ilvI at 1716.303 kb on - strand, within ilvI at 1716.303 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.327 kb on - strand, within ilvI at 1716.328 kb on + strand, within ilvI at 1716.328 kb on + strand, within ilvI at 1716.328 kb on + strand, within ilvI at 1716.328 kb on + strand, within ilvI at 1716.328 kb on + strand, within ilvI at 1716.329 kb on - strand, within ilvI at 1716.368 kb on + strand, within ilvI at 1716.369 kb on - strand, within ilvI at 1716.565 kb on - strand at 1716.578 kb on + strand at 1716.699 kb on + strand, within ilvN at 1716.700 kb on - strand, within ilvN at 1716.755 kb on + strand, within ilvN at 1716.756 kb on - strand, within ilvN at 1716.873 kb on + strand, within ilvN at 1716.874 kb on - strand, within ilvN at 1716.884 kb on + strand, within ilvN at 1716.923 kb on - strand, within ilvN at 1716.936 kb on - strand, within ilvN at 1716.952 kb on - strand, within ilvN at 1717.046 kb on + strand at 1717.133 kb on + strand, within fruL at 1717.134 kb on - strand, within fruL at 1717.167 kb on - strand at 1717.167 kb on - strand at 1717.196 kb on - strand at 1717.220 kb on + strand at 1717.290 kb on + strand at 1717.290 kb on + strand at 1717.290 kb on + strand at 1717.290 kb on + strand at 1717.290 kb on + strand at 1717.291 kb on - strand at 1717.295 kb on + strand at 1717.295 kb on + strand at 1717.296 kb on - strand at 1717.461 kb on + strand, within cra at 1717.462 kb on - strand, within cra at 1717.479 kb on + strand, within cra at 1717.480 kb on - strand, within cra at 1717.519 kb on + strand, within cra at 1717.635 kb on + strand, within cra at 1717.756 kb on + strand, within cra at 1717.757 kb on - strand, within cra at 1717.757 kb on - strand, within cra at 1718.002 kb on + strand, within cra at 1718.008 kb on + strand, within cra at 1718.009 kb on - strand, within cra at 1718.009 kb on - strand, within cra at 1718.022 kb on - strand, within cra at 1718.080 kb on + strand, within cra at 1718.205 kb on - strand at 1718.238 kb on + strand at 1718.319 kb on + strand at 1718.320 kb on - strand at 1718.324 kb on - strand at 1718.324 kb on - strand at 1718.339 kb on + strand at 1718.342 kb on + strand at 1718.348 kb on + strand at 1718.349 kb on - strand at 1718.440 kb on + strand at 1718.550 kb on + strand at 1718.551 kb on - strand at 1718.551 kb on - strand at 1718.589 kb on + strand at 1718.591 kb on + strand at 1718.659 kb on - strand at 1718.694 kb on - strand at 1718.715 kb on - strand at 1719.068 kb on + strand, within mraZ at 1719.068 kb on + strand, within mraZ at 1719.074 kb on + strand, within mraZ
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,716,302 + ilvI BNILDI_08565 0.85 +0.4 1,716,303 - ilvI BNILDI_08565 0.85 -0.1 1,716,303 - ilvI BNILDI_08565 0.85 +0.0 1,716,327 - ilvI BNILDI_08565 0.86 +0.9 1,716,327 - ilvI BNILDI_08565 0.86 +1.1 1,716,327 - ilvI BNILDI_08565 0.86 +0.2 1,716,327 - ilvI BNILDI_08565 0.86 -0.4 1,716,327 - ilvI BNILDI_08565 0.86 +0.7 1,716,327 - ilvI BNILDI_08565 0.86 +0.5 1,716,327 - ilvI BNILDI_08565 0.86 +1.3 1,716,328 + ilvI BNILDI_08565 0.86 -0.3 1,716,328 + ilvI BNILDI_08565 0.86 -0.7 1,716,328 + ilvI BNILDI_08565 0.86 -0.0 1,716,328 + ilvI BNILDI_08565 0.86 -0.3 1,716,328 + ilvI BNILDI_08565 0.86 -0.1 1,716,329 - ilvI BNILDI_08565 0.86 -1.8 1,716,368 + ilvI BNILDI_08565 0.89 -0.7 1,716,369 - ilvI BNILDI_08565 0.89 +1.2 1,716,565 - -0.1 1,716,578 + +0.3 1,716,699 + ilvN BNILDI_08570 0.27 -0.1 1,716,700 - ilvN BNILDI_08570 0.27 -1.7 1,716,755 + ilvN BNILDI_08570 0.39 +0.4 1,716,756 - ilvN BNILDI_08570 0.39 +0.5 1,716,873 + ilvN BNILDI_08570 0.63 -0.8 1,716,874 - ilvN BNILDI_08570 0.63 -0.3 1,716,884 + ilvN BNILDI_08570 0.65 +0.4 1,716,923 - ilvN BNILDI_08570 0.73 +2.7 1,716,936 - ilvN BNILDI_08570 0.75 -0.4 1,716,952 - ilvN BNILDI_08570 0.79 +0.6 1,717,046 + +0.8 1,717,133 + fruL BNILDI_08575 0.67 +0.2 1,717,134 - fruL BNILDI_08575 0.68 -0.8 1,717,167 - -3.2 1,717,167 - -2.3 1,717,196 - -2.5 1,717,220 + -1.3 1,717,290 + -3.0 1,717,290 + -3.0 1,717,290 + -3.6 1,717,290 + -3.0 1,717,290 + -2.7 1,717,291 - -5.5 1,717,295 + -0.8 1,717,295 + -1.8 1,717,296 - -2.0 1,717,461 + cra BNILDI_08580 0.22 -4.3 1,717,462 - cra BNILDI_08580 0.22 -5.0 1,717,479 + cra BNILDI_08580 0.24 -4.0 1,717,480 - cra BNILDI_08580 0.24 -2.5 1,717,519 + cra BNILDI_08580 0.28 -2.6 1,717,635 + cra BNILDI_08580 0.40 -2.8 1,717,756 + cra BNILDI_08580 0.52 -2.5 1,717,757 - cra BNILDI_08580 0.52 -5.4 1,717,757 - cra BNILDI_08580 0.52 -3.7 1,718,002 + cra BNILDI_08580 0.76 -1.2 1,718,008 + cra BNILDI_08580 0.77 -2.1 1,718,009 - cra BNILDI_08580 0.77 -2.9 1,718,009 - cra BNILDI_08580 0.77 -3.9 1,718,022 - cra BNILDI_08580 0.78 -2.3 1,718,080 + cra BNILDI_08580 0.84 -4.2 1,718,205 - -5.6 1,718,238 + -0.7 1,718,319 + +0.3 1,718,320 - +0.1 1,718,324 - -1.0 1,718,324 - -0.3 1,718,339 + +0.3 1,718,342 + +0.1 1,718,348 + -0.5 1,718,349 - +0.1 1,718,440 + +0.2 1,718,550 + -0.8 1,718,551 - +0.3 1,718,551 - -0.4 1,718,589 + +0.0 1,718,591 + +0.9 1,718,659 - -2.9 1,718,694 - -0.2 1,718,715 - +1.1 1,719,068 + mraZ BNILDI_08585 0.49 -0.0 1,719,068 + mraZ BNILDI_08585 0.49 -0.7 1,719,074 + mraZ BNILDI_08585 0.51 +0.6
Or see this region's nucleotide sequence