Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuA and BNILDI_08550 overlap by 1 nucleotides BNILDI_08550 and leuL are separated by 92 nucleotides leuL and leuO are separated by 659 nucleotides
BNILDI_08545: leuA - 2-isopropylmalate synthase, at 1,710,075 to 1,711,166
leuA
BNILDI_08550: BNILDI_08550 - 2-isopropylmalate synthase, at 1,711,166 to 1,712,737
_08550
BNILDI_08555: leuL - leu operon leader peptide, at 1,712,830 to 1,712,916
leuL
BNILDI_08560: leuO - transcriptional regulator LeuO, at 1,713,576 to 1,714,520
leuO
Position (kb)
1711
1712
1713 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1710.178 kb on - strand at 1710.235 kb on + strand, within leuA at 1710.329 kb on - strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.494 kb on - strand, within leuA at 1710.494 kb on - strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.498 kb on - strand, within leuA at 1710.667 kb on + strand, within leuA at 1710.667 kb on + strand, within leuA at 1710.668 kb on - strand, within leuA at 1710.668 kb on - strand, within leuA at 1710.694 kb on + strand, within leuA at 1710.733 kb on + strand, within leuA at 1710.855 kb on + strand, within leuA at 1711.032 kb on + strand, within leuA at 1711.033 kb on - strand, within leuA at 1711.253 kb on + strand at 1711.253 kb on + strand at 1711.254 kb on - strand at 1711.329 kb on + strand, within BNILDI_08550 at 1711.329 kb on + strand, within BNILDI_08550 at 1711.330 kb on - strand, within BNILDI_08550 at 1711.330 kb on - strand, within BNILDI_08550 at 1711.366 kb on + strand, within BNILDI_08550 at 1711.393 kb on - strand, within BNILDI_08550 at 1711.413 kb on + strand, within BNILDI_08550 at 1711.413 kb on + strand, within BNILDI_08550 at 1711.414 kb on + strand, within BNILDI_08550 at 1711.440 kb on + strand, within BNILDI_08550 at 1711.555 kb on - strand, within BNILDI_08550 at 1711.555 kb on - strand, within BNILDI_08550 at 1711.700 kb on - strand, within BNILDI_08550 at 1711.701 kb on + strand, within BNILDI_08550 at 1711.702 kb on - strand, within BNILDI_08550 at 1711.904 kb on - strand, within BNILDI_08550 at 1711.918 kb on + strand, within BNILDI_08550 at 1711.922 kb on + strand, within BNILDI_08550 at 1711.923 kb on - strand, within BNILDI_08550 at 1711.978 kb on + strand, within BNILDI_08550 at 1712.084 kb on + strand, within BNILDI_08550 at 1712.084 kb on + strand, within BNILDI_08550 at 1712.131 kb on + strand, within BNILDI_08550 at 1712.132 kb on - strand, within BNILDI_08550 at 1712.132 kb on - strand, within BNILDI_08550 at 1712.144 kb on - strand, within BNILDI_08550 at 1712.161 kb on + strand, within BNILDI_08550 at 1712.161 kb on + strand, within BNILDI_08550 at 1712.165 kb on + strand, within BNILDI_08550 at 1712.176 kb on - strand, within BNILDI_08550 at 1712.302 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.367 kb on + strand, within BNILDI_08550 at 1712.368 kb on - strand, within BNILDI_08550 at 1712.368 kb on - strand, within BNILDI_08550 at 1712.368 kb on - strand, within BNILDI_08550 at 1712.368 kb on - strand, within BNILDI_08550 at 1712.498 kb on - strand, within BNILDI_08550 at 1712.528 kb on + strand, within BNILDI_08550 at 1712.529 kb on - strand, within BNILDI_08550 at 1712.719 kb on + strand at 1712.781 kb on - strand at 1712.831 kb on + strand at 1712.856 kb on + strand, within leuL at 1712.882 kb on - strand, within leuL at 1712.917 kb on + strand at 1712.942 kb on - strand at 1712.942 kb on - strand at 1712.964 kb on - strand at 1713.267 kb on - strand at 1713.308 kb on + strand at 1713.553 kb on + strand at 1713.554 kb on - strand at 1713.571 kb on + strand at 1713.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,710,178 - -5.2 1,710,235 + leuA BNILDI_08545 0.15 -5.6 1,710,329 - leuA BNILDI_08545 0.23 -2.3 1,710,493 + leuA BNILDI_08545 0.38 -5.0 1,710,493 + leuA BNILDI_08545 0.38 -3.7 1,710,493 + leuA BNILDI_08545 0.38 -3.6 1,710,494 - leuA BNILDI_08545 0.38 -0.3 1,710,494 - leuA BNILDI_08545 0.38 -2.1 1,710,497 + leuA BNILDI_08545 0.39 -2.5 1,710,497 + leuA BNILDI_08545 0.39 -1.8 1,710,497 + leuA BNILDI_08545 0.39 -4.0 1,710,497 + leuA BNILDI_08545 0.39 -3.0 1,710,497 + leuA BNILDI_08545 0.39 -2.8 1,710,498 - leuA BNILDI_08545 0.39 -4.6 1,710,667 + leuA BNILDI_08545 0.54 -3.2 1,710,667 + leuA BNILDI_08545 0.54 -4.6 1,710,668 - leuA BNILDI_08545 0.54 -3.8 1,710,668 - leuA BNILDI_08545 0.54 -3.9 1,710,694 + leuA BNILDI_08545 0.57 -5.7 1,710,733 + leuA BNILDI_08545 0.60 -4.2 1,710,855 + leuA BNILDI_08545 0.71 -2.8 1,711,032 + leuA BNILDI_08545 0.88 -3.4 1,711,033 - leuA BNILDI_08545 0.88 -4.3 1,711,253 + -4.0 1,711,253 + -3.4 1,711,254 - -1.7 1,711,329 + BNILDI_08550 0.10 -4.0 1,711,329 + BNILDI_08550 0.10 -4.1 1,711,330 - BNILDI_08550 0.10 -2.1 1,711,330 - BNILDI_08550 0.10 -1.4 1,711,366 + BNILDI_08550 0.13 -1.9 1,711,393 - BNILDI_08550 0.14 -1.8 1,711,413 + BNILDI_08550 0.16 -1.8 1,711,413 + BNILDI_08550 0.16 -3.2 1,711,414 + BNILDI_08550 0.16 -2.5 1,711,440 + BNILDI_08550 0.17 -2.4 1,711,555 - BNILDI_08550 0.25 +0.3 1,711,555 - BNILDI_08550 0.25 -0.8 1,711,700 - BNILDI_08550 0.34 -6.3 1,711,701 + BNILDI_08550 0.34 -3.3 1,711,702 - BNILDI_08550 0.34 -1.4 1,711,904 - BNILDI_08550 0.47 -5.1 1,711,918 + BNILDI_08550 0.48 -5.4 1,711,922 + BNILDI_08550 0.48 -4.0 1,711,923 - BNILDI_08550 0.48 -5.7 1,711,978 + BNILDI_08550 0.52 -2.8 1,712,084 + BNILDI_08550 0.58 -3.6 1,712,084 + BNILDI_08550 0.58 -3.2 1,712,131 + BNILDI_08550 0.61 -3.5 1,712,132 - BNILDI_08550 0.61 -6.2 1,712,132 - BNILDI_08550 0.61 -4.9 1,712,144 - BNILDI_08550 0.62 -5.5 1,712,161 + BNILDI_08550 0.63 -3.6 1,712,161 + BNILDI_08550 0.63 -4.5 1,712,165 + BNILDI_08550 0.64 -5.2 1,712,176 - BNILDI_08550 0.64 -3.4 1,712,302 + BNILDI_08550 0.72 -5.0 1,712,367 + BNILDI_08550 0.76 -4.2 1,712,367 + BNILDI_08550 0.76 -4.2 1,712,367 + BNILDI_08550 0.76 -5.1 1,712,367 + BNILDI_08550 0.76 -1.3 1,712,367 + BNILDI_08550 0.76 -1.3 1,712,367 + BNILDI_08550 0.76 -5.2 1,712,368 - BNILDI_08550 0.76 -3.6 1,712,368 - BNILDI_08550 0.76 -3.3 1,712,368 - BNILDI_08550 0.76 -2.8 1,712,368 - BNILDI_08550 0.76 -2.7 1,712,498 - BNILDI_08550 0.85 -5.0 1,712,528 + BNILDI_08550 0.87 -5.1 1,712,529 - BNILDI_08550 0.87 -4.1 1,712,719 + -3.5 1,712,781 - -0.7 1,712,831 + -2.8 1,712,856 + leuL BNILDI_08555 0.30 -5.5 1,712,882 - leuL BNILDI_08555 0.60 -0.8 1,712,917 + -3.7 1,712,942 - +0.6 1,712,942 - -0.7 1,712,964 - -2.1 1,713,267 - +0.4 1,713,308 + +0.3 1,713,553 + -0.2 1,713,554 - -0.1 1,713,571 + +0.1 1,713,572 - -0.2
Or see this region's nucleotide sequence