Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC1 and leuA are separated by 2 nucleotides leuA and BNILDI_08550 overlap by 1 nucleotides
BNILDI_08540: leuC1 - 3-isopropylmalate dehydratase large subunit 1, at 1,708,672 to 1,710,072
leuC1
BNILDI_08545: leuA - 2-isopropylmalate synthase, at 1,710,075 to 1,711,166
leuA
BNILDI_08550: BNILDI_08550 - 2-isopropylmalate synthase, at 1,711,166 to 1,712,737
_08550
Position (kb)
1710
1711
1712 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1709.099 kb on + strand, within leuC1 at 1709.100 kb on - strand, within leuC1 at 1709.100 kb on - strand, within leuC1 at 1709.163 kb on + strand, within leuC1 at 1709.292 kb on - strand, within leuC1 at 1709.352 kb on + strand, within leuC1 at 1709.352 kb on + strand, within leuC1 at 1709.352 kb on + strand, within leuC1 at 1709.352 kb on + strand, within leuC1 at 1709.353 kb on - strand, within leuC1 at 1709.353 kb on - strand, within leuC1 at 1709.353 kb on - strand, within leuC1 at 1709.410 kb on - strand, within leuC1 at 1709.425 kb on - strand, within leuC1 at 1709.617 kb on + strand, within leuC1 at 1709.617 kb on + strand, within leuC1 at 1709.618 kb on - strand, within leuC1 at 1709.618 kb on - strand, within leuC1 at 1709.618 kb on - strand, within leuC1 at 1709.744 kb on - strand, within leuC1 at 1709.812 kb on - strand, within leuC1 at 1709.994 kb on + strand at 1709.994 kb on + strand at 1709.995 kb on - strand at 1709.996 kb on + strand at 1709.997 kb on - strand at 1709.999 kb on - strand at 1710.027 kb on - strand at 1710.027 kb on - strand at 1710.031 kb on + strand at 1710.031 kb on + strand at 1710.031 kb on + strand at 1710.076 kb on + strand at 1710.076 kb on + strand at 1710.077 kb on - strand at 1710.125 kb on + strand at 1710.178 kb on - strand at 1710.235 kb on + strand, within leuA at 1710.329 kb on - strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.493 kb on + strand, within leuA at 1710.494 kb on - strand, within leuA at 1710.494 kb on - strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.497 kb on + strand, within leuA at 1710.498 kb on - strand, within leuA at 1710.667 kb on + strand, within leuA at 1710.667 kb on + strand, within leuA at 1710.668 kb on - strand, within leuA at 1710.668 kb on - strand, within leuA at 1710.694 kb on + strand, within leuA at 1710.733 kb on + strand, within leuA at 1710.855 kb on + strand, within leuA at 1711.032 kb on + strand, within leuA at 1711.033 kb on - strand, within leuA at 1711.253 kb on + strand at 1711.253 kb on + strand at 1711.254 kb on - strand at 1711.329 kb on + strand, within BNILDI_08550 at 1711.329 kb on + strand, within BNILDI_08550 at 1711.330 kb on - strand, within BNILDI_08550 at 1711.330 kb on - strand, within BNILDI_08550 at 1711.366 kb on + strand, within BNILDI_08550 at 1711.393 kb on - strand, within BNILDI_08550 at 1711.413 kb on + strand, within BNILDI_08550 at 1711.413 kb on + strand, within BNILDI_08550 at 1711.414 kb on + strand, within BNILDI_08550 at 1711.440 kb on + strand, within BNILDI_08550 at 1711.555 kb on - strand, within BNILDI_08550 at 1711.555 kb on - strand, within BNILDI_08550 at 1711.700 kb on - strand, within BNILDI_08550 at 1711.701 kb on + strand, within BNILDI_08550 at 1711.702 kb on - strand, within BNILDI_08550 at 1711.904 kb on - strand, within BNILDI_08550 at 1711.918 kb on + strand, within BNILDI_08550 at 1711.922 kb on + strand, within BNILDI_08550 at 1711.923 kb on - strand, within BNILDI_08550 at 1711.978 kb on + strand, within BNILDI_08550 at 1712.084 kb on + strand, within BNILDI_08550 at 1712.084 kb on + strand, within BNILDI_08550 at 1712.131 kb on + strand, within BNILDI_08550 at 1712.132 kb on - strand, within BNILDI_08550 at 1712.132 kb on - strand, within BNILDI_08550 at 1712.144 kb on - strand, within BNILDI_08550 at 1712.161 kb on + strand, within BNILDI_08550 at 1712.161 kb on + strand, within BNILDI_08550 at 1712.165 kb on + strand, within BNILDI_08550
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,709,099 + leuC1 BNILDI_08540 0.30 -3.8 1,709,100 - leuC1 BNILDI_08540 0.31 -4.0 1,709,100 - leuC1 BNILDI_08540 0.31 -2.9 1,709,163 + leuC1 BNILDI_08540 0.35 -5.0 1,709,292 - leuC1 BNILDI_08540 0.44 -5.5 1,709,352 + leuC1 BNILDI_08540 0.49 -4.2 1,709,352 + leuC1 BNILDI_08540 0.49 -3.1 1,709,352 + leuC1 BNILDI_08540 0.49 -5.1 1,709,352 + leuC1 BNILDI_08540 0.49 -7.0 1,709,353 - leuC1 BNILDI_08540 0.49 -3.6 1,709,353 - leuC1 BNILDI_08540 0.49 -1.8 1,709,353 - leuC1 BNILDI_08540 0.49 -2.7 1,709,410 - leuC1 BNILDI_08540 0.53 -5.9 1,709,425 - leuC1 BNILDI_08540 0.54 -2.5 1,709,617 + leuC1 BNILDI_08540 0.67 -2.2 1,709,617 + leuC1 BNILDI_08540 0.67 -3.2 1,709,618 - leuC1 BNILDI_08540 0.68 -4.4 1,709,618 - leuC1 BNILDI_08540 0.68 -1.7 1,709,618 - leuC1 BNILDI_08540 0.68 -4.0 1,709,744 - leuC1 BNILDI_08540 0.77 -2.6 1,709,812 - leuC1 BNILDI_08540 0.81 -3.5 1,709,994 + -3.2 1,709,994 + -1.5 1,709,995 - -4.4 1,709,996 + -3.5 1,709,997 - -3.1 1,709,999 - -7.4 1,710,027 - -3.0 1,710,027 - -3.6 1,710,031 + -3.3 1,710,031 + -2.0 1,710,031 + -4.3 1,710,076 + -0.3 1,710,076 + -7.2 1,710,077 - -2.5 1,710,125 + -4.1 1,710,178 - -5.2 1,710,235 + leuA BNILDI_08545 0.15 -5.6 1,710,329 - leuA BNILDI_08545 0.23 -2.3 1,710,493 + leuA BNILDI_08545 0.38 -5.0 1,710,493 + leuA BNILDI_08545 0.38 -3.7 1,710,493 + leuA BNILDI_08545 0.38 -3.6 1,710,494 - leuA BNILDI_08545 0.38 -0.3 1,710,494 - leuA BNILDI_08545 0.38 -2.1 1,710,497 + leuA BNILDI_08545 0.39 -2.5 1,710,497 + leuA BNILDI_08545 0.39 -1.8 1,710,497 + leuA BNILDI_08545 0.39 -4.0 1,710,497 + leuA BNILDI_08545 0.39 -3.0 1,710,497 + leuA BNILDI_08545 0.39 -2.8 1,710,498 - leuA BNILDI_08545 0.39 -4.6 1,710,667 + leuA BNILDI_08545 0.54 -3.2 1,710,667 + leuA BNILDI_08545 0.54 -4.6 1,710,668 - leuA BNILDI_08545 0.54 -3.8 1,710,668 - leuA BNILDI_08545 0.54 -3.9 1,710,694 + leuA BNILDI_08545 0.57 -5.7 1,710,733 + leuA BNILDI_08545 0.60 -4.2 1,710,855 + leuA BNILDI_08545 0.71 -2.8 1,711,032 + leuA BNILDI_08545 0.88 -3.4 1,711,033 - leuA BNILDI_08545 0.88 -4.3 1,711,253 + -4.0 1,711,253 + -3.4 1,711,254 - -1.7 1,711,329 + BNILDI_08550 0.10 -4.0 1,711,329 + BNILDI_08550 0.10 -4.1 1,711,330 - BNILDI_08550 0.10 -2.1 1,711,330 - BNILDI_08550 0.10 -1.4 1,711,366 + BNILDI_08550 0.13 -1.9 1,711,393 - BNILDI_08550 0.14 -1.8 1,711,413 + BNILDI_08550 0.16 -1.8 1,711,413 + BNILDI_08550 0.16 -3.2 1,711,414 + BNILDI_08550 0.16 -2.5 1,711,440 + BNILDI_08550 0.17 -2.4 1,711,555 - BNILDI_08550 0.25 +0.3 1,711,555 - BNILDI_08550 0.25 -0.8 1,711,700 - BNILDI_08550 0.34 -6.3 1,711,701 + BNILDI_08550 0.34 -3.3 1,711,702 - BNILDI_08550 0.34 -1.4 1,711,904 - BNILDI_08550 0.47 -5.1 1,711,918 + BNILDI_08550 0.48 -5.4 1,711,922 + BNILDI_08550 0.48 -4.0 1,711,923 - BNILDI_08550 0.48 -5.7 1,711,978 + BNILDI_08550 0.52 -2.8 1,712,084 + BNILDI_08550 0.58 -3.6 1,712,084 + BNILDI_08550 0.58 -3.2 1,712,131 + BNILDI_08550 0.61 -3.5 1,712,132 - BNILDI_08550 0.61 -6.2 1,712,132 - BNILDI_08550 0.61 -4.9 1,712,144 - BNILDI_08550 0.62 -5.5 1,712,161 + BNILDI_08550 0.63 -3.6 1,712,161 + BNILDI_08550 0.63 -4.5 1,712,165 + BNILDI_08550 0.64 -5.2
Or see this region's nucleotide sequence