Strain Fitness in Escherichia coli ECRC62 around BNILDI_08435

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrsmA and pdxA overlap by 4 nucleotidespdxA and surA overlap by 1 nucleotidessurA and lptD are separated by 52 nucleotides BNILDI_08425: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 1,682,293 to 1,683,114 rsmA BNILDI_08430: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 1,683,111 to 1,684,100 pdxA BNILDI_08435: surA - peptidylprolyl isomerase SurA, at 1,684,100 to 1,685,386 surA BNILDI_08440: lptD - LPS assembly protein LptD, at 1,685,439 to 1,687,793 lptD Position (kb) 1684 1685 1686Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1683.249 kb on - strand, within pdxAat 1683.283 kb on - strand, within pdxAat 1683.449 kb on + strand, within pdxAat 1683.543 kb on - strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.814 kb on + strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.815 kb on - strand, within pdxAat 1683.876 kb on + strand, within pdxAat 1683.877 kb on - strand, within pdxAat 1683.877 kb on - strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.925 kb on + strand, within pdxAat 1683.926 kb on - strand, within pdxAat 1684.175 kb on - strandat 1684.257 kb on - strand, within surAat 1684.257 kb on - strand, within surAat 1684.435 kb on + strand, within surAat 1684.436 kb on - strand, within surAat 1684.436 kb on - strand, within surAat 1684.647 kb on - strand, within surAat 1684.976 kb on - strand, within surAat 1685.004 kb on + strand, within surAat 1685.013 kb on + strand, within surAat 1685.013 kb on + strand, within surAat 1685.014 kb on - strand, within surAat 1685.014 kb on - strand, within surAat 1685.029 kb on - strand, within surAat 1685.386 kb on + strandat 1685.405 kb on - strandat 1685.420 kb on + strandat 1685.421 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,683,249 - pdxA BNILDI_08430 0.14 +1.4
1,683,283 - pdxA BNILDI_08430 0.17 -2.6
1,683,449 + pdxA BNILDI_08430 0.34 -3.1
1,683,543 - pdxA BNILDI_08430 0.44 -2.2
1,683,814 + pdxA BNILDI_08430 0.71 -0.3
1,683,814 + pdxA BNILDI_08430 0.71 -2.5
1,683,814 + pdxA BNILDI_08430 0.71 -1.9
1,683,815 - pdxA BNILDI_08430 0.71 -4.7
1,683,815 - pdxA BNILDI_08430 0.71 -2.8
1,683,815 - pdxA BNILDI_08430 0.71 -1.4
1,683,876 + pdxA BNILDI_08430 0.77 -2.3
1,683,877 - pdxA BNILDI_08430 0.77 -1.2
1,683,877 - pdxA BNILDI_08430 0.77 -2.4
1,683,925 + pdxA BNILDI_08430 0.82 -0.9
1,683,925 + pdxA BNILDI_08430 0.82 -3.1
1,683,925 + pdxA BNILDI_08430 0.82 -0.2
1,683,926 - pdxA BNILDI_08430 0.82 -1.5
1,684,175 - +0.1
1,684,257 - surA BNILDI_08435 0.12 +1.4
1,684,257 - surA BNILDI_08435 0.12 +1.3
1,684,435 + surA BNILDI_08435 0.26 -2.3
1,684,436 - surA BNILDI_08435 0.26 -0.7
1,684,436 - surA BNILDI_08435 0.26 +0.8
1,684,647 - surA BNILDI_08435 0.43 -0.1
1,684,976 - surA BNILDI_08435 0.68 -2.1
1,685,004 + surA BNILDI_08435 0.70 -2.2
1,685,013 + surA BNILDI_08435 0.71 -2.7
1,685,013 + surA BNILDI_08435 0.71 -3.6
1,685,014 - surA BNILDI_08435 0.71 -1.4
1,685,014 - surA BNILDI_08435 0.71 +1.1
1,685,029 - surA BNILDI_08435 0.72 -3.8
1,685,386 + -2.6
1,685,405 - +0.2
1,685,420 + -1.1
1,685,421 - -0.3

Or see this region's nucleotide sequence