Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmA and pdxA overlap by 4 nucleotides pdxA and surA overlap by 1 nucleotides surA and lptD are separated by 52 nucleotides
BNILDI_08425: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 1,682,293 to 1,683,114
rsmA
BNILDI_08430: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 1,683,111 to 1,684,100
pdxA
BNILDI_08435: surA - peptidylprolyl isomerase SurA, at 1,684,100 to 1,685,386
surA
BNILDI_08440: lptD - LPS assembly protein LptD, at 1,685,439 to 1,687,793
lptD
Position (kb)
1684
1685
1686 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1683.249 kb on - strand, within pdxA at 1683.283 kb on - strand, within pdxA at 1683.449 kb on + strand, within pdxA at 1683.543 kb on - strand, within pdxA at 1683.814 kb on + strand, within pdxA at 1683.814 kb on + strand, within pdxA at 1683.814 kb on + strand, within pdxA at 1683.815 kb on - strand, within pdxA at 1683.815 kb on - strand, within pdxA at 1683.815 kb on - strand, within pdxA at 1683.876 kb on + strand, within pdxA at 1683.877 kb on - strand, within pdxA at 1683.877 kb on - strand, within pdxA at 1683.925 kb on + strand, within pdxA at 1683.925 kb on + strand, within pdxA at 1683.925 kb on + strand, within pdxA at 1683.926 kb on - strand, within pdxA at 1684.175 kb on - strand at 1684.257 kb on - strand, within surA at 1684.257 kb on - strand, within surA at 1684.435 kb on + strand, within surA at 1684.436 kb on - strand, within surA at 1684.436 kb on - strand, within surA at 1684.647 kb on - strand, within surA at 1684.976 kb on - strand, within surA at 1685.004 kb on + strand, within surA at 1685.013 kb on + strand, within surA at 1685.013 kb on + strand, within surA at 1685.014 kb on - strand, within surA at 1685.014 kb on - strand, within surA at 1685.029 kb on - strand, within surA at 1685.386 kb on + strand at 1685.405 kb on - strand at 1685.420 kb on + strand at 1685.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,683,249 - pdxA BNILDI_08430 0.14 +1.4 1,683,283 - pdxA BNILDI_08430 0.17 -2.6 1,683,449 + pdxA BNILDI_08430 0.34 -3.1 1,683,543 - pdxA BNILDI_08430 0.44 -2.2 1,683,814 + pdxA BNILDI_08430 0.71 -0.3 1,683,814 + pdxA BNILDI_08430 0.71 -2.5 1,683,814 + pdxA BNILDI_08430 0.71 -1.9 1,683,815 - pdxA BNILDI_08430 0.71 -4.7 1,683,815 - pdxA BNILDI_08430 0.71 -2.8 1,683,815 - pdxA BNILDI_08430 0.71 -1.4 1,683,876 + pdxA BNILDI_08430 0.77 -2.3 1,683,877 - pdxA BNILDI_08430 0.77 -1.2 1,683,877 - pdxA BNILDI_08430 0.77 -2.4 1,683,925 + pdxA BNILDI_08430 0.82 -0.9 1,683,925 + pdxA BNILDI_08430 0.82 -3.1 1,683,925 + pdxA BNILDI_08430 0.82 -0.2 1,683,926 - pdxA BNILDI_08430 0.82 -1.5 1,684,175 - +0.1 1,684,257 - surA BNILDI_08435 0.12 +1.4 1,684,257 - surA BNILDI_08435 0.12 +1.3 1,684,435 + surA BNILDI_08435 0.26 -2.3 1,684,436 - surA BNILDI_08435 0.26 -0.7 1,684,436 - surA BNILDI_08435 0.26 +0.8 1,684,647 - surA BNILDI_08435 0.43 -0.1 1,684,976 - surA BNILDI_08435 0.68 -2.1 1,685,004 + surA BNILDI_08435 0.70 -2.2 1,685,013 + surA BNILDI_08435 0.71 -2.7 1,685,013 + surA BNILDI_08435 0.71 -3.6 1,685,014 - surA BNILDI_08435 0.71 -1.4 1,685,014 - surA BNILDI_08435 0.71 +1.1 1,685,029 - surA BNILDI_08435 0.72 -3.8 1,685,386 + -2.6 1,685,405 - +0.2 1,685,420 + -1.1 1,685,421 - -0.3
Or see this region's nucleotide sequence