Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides thrC and yaaX are separated by 213 nucleotides yaaX and BNILDI_08180 are separated by 259 nucleotides
BNILDI_08160: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 1,627,791 to 1,630,253
thrA
BNILDI_08165: thrB - homoserine kinase, at 1,630,255 to 1,631,187
thrB
BNILDI_08170: thrC - threonine synthase, at 1,631,188 to 1,632,474
thrC
BNILDI_08175: yaaX - Uncharacterized protein YaaX, at 1,632,688 to 1,632,984
yaaX
BNILDI_08180: BNILDI_08180 - IS110-like element IS621 family transposase, at 1,633,244 to 1,634,224
_08180
Position (kb) 1631
1632
1633 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1630.193 kb on + strand at 1630.222 kb on + strand at 1630.222 kb on + strand at 1630.223 kb on - strand at 1630.226 kb on + strand at 1630.226 kb on + strand at 1630.244 kb on - strand at 1630.260 kb on + strand at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.443 kb on + strand, within thrB at 1630.444 kb on - strand, within thrB at 1630.522 kb on - strand, within thrB at 1630.542 kb on - strand, within thrB at 1630.821 kb on + strand, within thrB at 1630.897 kb on + strand, within thrB at 1630.941 kb on + strand, within thrB at 1630.954 kb on + strand, within thrB at 1631.061 kb on - strand, within thrB at 1631.114 kb on - strand at 1631.143 kb on - strand at 1631.197 kb on + strand at 1631.624 kb on - strand, within thrC at 1631.701 kb on - strand, within thrC at 1631.820 kb on + strand, within thrC at 1631.830 kb on - strand, within thrC at 1632.295 kb on + strand, within thrC at 1632.296 kb on - strand, within thrC at 1632.404 kb on + strand at 1632.404 kb on + strand at 1632.420 kb on + strand at 1632.420 kb on + strand at 1632.472 kb on + strand at 1632.479 kb on + strand at 1632.487 kb on - strand at 1632.512 kb on + strand at 1632.594 kb on + strand at 1632.673 kb on + strand at 1632.674 kb on - strand at 1632.705 kb on + strand at 1632.706 kb on - strand at 1632.706 kb on - strand at 1632.710 kb on + strand at 1632.765 kb on + strand, within yaaX at 1632.765 kb on + strand, within yaaX at 1632.795 kb on - strand, within yaaX at 1632.820 kb on + strand, within yaaX at 1632.820 kb on + strand, within yaaX at 1632.868 kb on + strand, within yaaX at 1632.868 kb on + strand, within yaaX at 1632.928 kb on - strand, within yaaX at 1632.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,630,193 + -3.3 1,630,222 + -4.1 1,630,222 + -2.5 1,630,223 - -3.4 1,630,226 + -2.7 1,630,226 + -1.2 1,630,244 - -3.3 1,630,260 + -2.5 1,630,358 + thrB BNILDI_08165 0.11 -2.2 1,630,358 + thrB BNILDI_08165 0.11 -2.3 1,630,358 + thrB BNILDI_08165 0.11 +0.2 1,630,358 + thrB BNILDI_08165 0.11 -2.0 1,630,359 - thrB BNILDI_08165 0.11 -5.9 1,630,359 - thrB BNILDI_08165 0.11 -4.5 1,630,359 - thrB BNILDI_08165 0.11 -3.5 1,630,443 + thrB BNILDI_08165 0.20 -3.4 1,630,444 - thrB BNILDI_08165 0.20 -3.0 1,630,522 - thrB BNILDI_08165 0.29 -3.9 1,630,542 - thrB BNILDI_08165 0.31 -4.0 1,630,821 + thrB BNILDI_08165 0.61 -1.1 1,630,897 + thrB BNILDI_08165 0.69 -2.4 1,630,941 + thrB BNILDI_08165 0.74 -2.7 1,630,954 + thrB BNILDI_08165 0.75 -3.9 1,631,061 - thrB BNILDI_08165 0.86 -1.3 1,631,114 - -3.2 1,631,143 - -3.2 1,631,197 + -3.9 1,631,624 - thrC BNILDI_08170 0.34 -3.3 1,631,701 - thrC BNILDI_08170 0.40 -2.3 1,631,820 + thrC BNILDI_08170 0.49 -3.8 1,631,830 - thrC BNILDI_08170 0.50 -2.4 1,632,295 + thrC BNILDI_08170 0.86 -0.6 1,632,296 - thrC BNILDI_08170 0.86 -3.6 1,632,404 + -1.5 1,632,404 + -3.1 1,632,420 + -6.4 1,632,420 + -1.1 1,632,472 + +0.6 1,632,479 + +1.3 1,632,487 - -0.0 1,632,512 + +0.1 1,632,594 + -3.1 1,632,673 + -0.4 1,632,674 - +0.6 1,632,705 + +1.7 1,632,706 - -0.7 1,632,706 - -0.3 1,632,710 + +1.5 1,632,765 + yaaX BNILDI_08175 0.26 +0.7 1,632,765 + yaaX BNILDI_08175 0.26 +0.2 1,632,795 - yaaX BNILDI_08175 0.36 +1.0 1,632,820 + yaaX BNILDI_08175 0.44 -0.5 1,632,820 + yaaX BNILDI_08175 0.44 -1.4 1,632,868 + yaaX BNILDI_08175 0.61 +1.0 1,632,868 + yaaX BNILDI_08175 0.61 -0.2 1,632,928 - yaaX BNILDI_08175 0.81 +0.1 1,632,982 + -1.3
Or see this region's nucleotide sequence