Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lasT and BNILDI_08155 are separated by 213 nucleotides BNILDI_08155 and thrA are separated by 80 nucleotides thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
BNILDI_08150: lasT - tRNA/rRNA methyltransferase, at 1,626,745 to 1,627,431
lasT
BNILDI_08155: BNILDI_08155 - thr operon leader peptide, at 1,627,645 to 1,627,710
_08155
BNILDI_08160: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 1,627,791 to 1,630,253
thrA
BNILDI_08165: thrB - homoserine kinase, at 1,630,255 to 1,631,187
thrB
BNILDI_08170: thrC - threonine synthase, at 1,631,188 to 1,632,474
thrC
Position (kb)
1627
1628
1629
1630
1631 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1626.828 kb on + strand, within lasT at 1626.997 kb on + strand, within lasT at 1627.003 kb on + strand, within lasT at 1627.004 kb on - strand, within lasT at 1627.004 kb on - strand, within lasT at 1627.116 kb on - strand, within lasT at 1627.134 kb on + strand, within lasT at 1627.135 kb on - strand, within lasT at 1627.325 kb on + strand, within lasT at 1627.325 kb on + strand, within lasT at 1627.325 kb on + strand, within lasT at 1627.325 kb on + strand, within lasT at 1627.326 kb on - strand, within lasT at 1627.326 kb on - strand, within lasT at 1627.364 kb on + strand at 1627.365 kb on - strand at 1627.444 kb on + strand at 1627.445 kb on - strand at 1627.484 kb on + strand at 1627.484 kb on + strand at 1627.485 kb on - strand at 1627.519 kb on + strand at 1627.551 kb on + strand at 1627.552 kb on - strand at 1627.620 kb on - strand at 1627.668 kb on + strand, within BNILDI_08155 at 1627.668 kb on + strand, within BNILDI_08155 at 1627.783 kb on + strand at 1627.784 kb on - strand at 1627.784 kb on - strand at 1628.092 kb on + strand, within thrA at 1628.093 kb on - strand, within thrA at 1628.093 kb on - strand, within thrA at 1628.093 kb on - strand, within thrA at 1628.114 kb on + strand, within thrA at 1628.114 kb on + strand, within thrA at 1628.115 kb on - strand, within thrA at 1628.115 kb on - strand, within thrA at 1628.115 kb on - strand, within thrA at 1628.115 kb on - strand, within thrA at 1628.115 kb on - strand, within thrA at 1628.268 kb on + strand, within thrA at 1628.269 kb on - strand, within thrA at 1628.487 kb on + strand, within thrA at 1628.487 kb on + strand, within thrA at 1628.488 kb on - strand, within thrA at 1628.489 kb on + strand, within thrA at 1628.666 kb on + strand, within thrA at 1628.729 kb on + strand, within thrA at 1628.856 kb on + strand, within thrA at 1628.861 kb on + strand, within thrA at 1628.925 kb on + strand, within thrA at 1628.995 kb on + strand, within thrA at 1628.996 kb on - strand, within thrA at 1628.996 kb on - strand, within thrA at 1629.006 kb on - strand, within thrA at 1629.062 kb on + strand, within thrA at 1629.122 kb on + strand, within thrA at 1629.270 kb on - strand, within thrA at 1629.283 kb on + strand, within thrA at 1629.284 kb on - strand, within thrA at 1629.284 kb on - strand, within thrA at 1629.421 kb on - strand, within thrA at 1629.481 kb on - strand, within thrA at 1629.552 kb on + strand, within thrA at 1629.552 kb on + strand, within thrA at 1629.552 kb on + strand, within thrA at 1629.553 kb on - strand, within thrA at 1629.555 kb on + strand, within thrA at 1629.556 kb on - strand, within thrA at 1629.715 kb on - strand, within thrA at 1629.715 kb on - strand, within thrA at 1629.716 kb on + strand, within thrA at 1629.716 kb on + strand, within thrA at 1629.717 kb on - strand, within thrA at 1629.717 kb on - strand, within thrA at 1629.730 kb on + strand, within thrA at 1630.035 kb on - strand at 1630.082 kb on + strand at 1630.131 kb on + strand at 1630.132 kb on - strand at 1630.141 kb on - strand at 1630.143 kb on + strand at 1630.193 kb on + strand at 1630.222 kb on + strand at 1630.222 kb on + strand at 1630.223 kb on - strand at 1630.226 kb on + strand at 1630.226 kb on + strand at 1630.244 kb on - strand at 1630.260 kb on + strand at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.358 kb on + strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.359 kb on - strand, within thrB at 1630.443 kb on + strand, within thrB at 1630.444 kb on - strand, within thrB at 1630.522 kb on - strand, within thrB at 1630.542 kb on - strand, within thrB at 1630.821 kb on + strand, within thrB at 1630.897 kb on + strand, within thrB at 1630.941 kb on + strand, within thrB at 1630.954 kb on + strand, within thrB at 1631.061 kb on - strand, within thrB at 1631.114 kb on - strand at 1631.143 kb on - strand at 1631.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,626,828 + lasT BNILDI_08150 0.12 -0.3 1,626,997 + lasT BNILDI_08150 0.37 -1.3 1,627,003 + lasT BNILDI_08150 0.38 +0.5 1,627,004 - lasT BNILDI_08150 0.38 -0.3 1,627,004 - lasT BNILDI_08150 0.38 -0.0 1,627,116 - lasT BNILDI_08150 0.54 +0.4 1,627,134 + lasT BNILDI_08150 0.57 +0.0 1,627,135 - lasT BNILDI_08150 0.57 -1.4 1,627,325 + lasT BNILDI_08150 0.84 -0.3 1,627,325 + lasT BNILDI_08150 0.84 -0.6 1,627,325 + lasT BNILDI_08150 0.84 +1.9 1,627,325 + lasT BNILDI_08150 0.84 -0.1 1,627,326 - lasT BNILDI_08150 0.85 +0.9 1,627,326 - lasT BNILDI_08150 0.85 +0.6 1,627,364 + -1.2 1,627,365 - +1.4 1,627,444 + -0.6 1,627,445 - -0.1 1,627,484 + +0.0 1,627,484 + +0.5 1,627,485 - +0.8 1,627,519 + -0.1 1,627,551 + +0.7 1,627,552 - +0.4 1,627,620 - -3.6 1,627,668 + BNILDI_08155 0.35 +0.5 1,627,668 + BNILDI_08155 0.35 -1.2 1,627,783 + -2.0 1,627,784 - -2.5 1,627,784 - -2.7 1,628,092 + thrA BNILDI_08160 0.12 -2.8 1,628,093 - thrA BNILDI_08160 0.12 -1.4 1,628,093 - thrA BNILDI_08160 0.12 -3.3 1,628,093 - thrA BNILDI_08160 0.12 -1.4 1,628,114 + thrA BNILDI_08160 0.13 -2.5 1,628,114 + thrA BNILDI_08160 0.13 -1.4 1,628,115 - thrA BNILDI_08160 0.13 -2.1 1,628,115 - thrA BNILDI_08160 0.13 -4.3 1,628,115 - thrA BNILDI_08160 0.13 -3.9 1,628,115 - thrA BNILDI_08160 0.13 -3.8 1,628,115 - thrA BNILDI_08160 0.13 -3.3 1,628,268 + thrA BNILDI_08160 0.19 -2.3 1,628,269 - thrA BNILDI_08160 0.19 -0.5 1,628,487 + thrA BNILDI_08160 0.28 -2.0 1,628,487 + thrA BNILDI_08160 0.28 -3.0 1,628,488 - thrA BNILDI_08160 0.28 -2.0 1,628,489 + thrA BNILDI_08160 0.28 -4.5 1,628,666 + thrA BNILDI_08160 0.36 -1.0 1,628,729 + thrA BNILDI_08160 0.38 -2.3 1,628,856 + thrA BNILDI_08160 0.43 -2.7 1,628,861 + thrA BNILDI_08160 0.43 -2.8 1,628,925 + thrA BNILDI_08160 0.46 -3.5 1,628,995 + thrA BNILDI_08160 0.49 -3.3 1,628,996 - thrA BNILDI_08160 0.49 -1.6 1,628,996 - thrA BNILDI_08160 0.49 -1.7 1,629,006 - thrA BNILDI_08160 0.49 -1.5 1,629,062 + thrA BNILDI_08160 0.52 -1.6 1,629,122 + thrA BNILDI_08160 0.54 -1.2 1,629,270 - thrA BNILDI_08160 0.60 -3.2 1,629,283 + thrA BNILDI_08160 0.61 -1.6 1,629,284 - thrA BNILDI_08160 0.61 -3.3 1,629,284 - thrA BNILDI_08160 0.61 -2.8 1,629,421 - thrA BNILDI_08160 0.66 -0.8 1,629,481 - thrA BNILDI_08160 0.69 -3.5 1,629,552 + thrA BNILDI_08160 0.71 -2.6 1,629,552 + thrA BNILDI_08160 0.71 -3.5 1,629,552 + thrA BNILDI_08160 0.71 -2.7 1,629,553 - thrA BNILDI_08160 0.72 -2.9 1,629,555 + thrA BNILDI_08160 0.72 -3.9 1,629,556 - thrA BNILDI_08160 0.72 -4.3 1,629,715 - thrA BNILDI_08160 0.78 -3.1 1,629,715 - thrA BNILDI_08160 0.78 -2.5 1,629,716 + thrA BNILDI_08160 0.78 -2.5 1,629,716 + thrA BNILDI_08160 0.78 -2.8 1,629,717 - thrA BNILDI_08160 0.78 -3.2 1,629,717 - thrA BNILDI_08160 0.78 -6.0 1,629,730 + thrA BNILDI_08160 0.79 -2.8 1,630,035 - -2.4 1,630,082 + -2.1 1,630,131 + -5.5 1,630,132 - -2.1 1,630,141 - -2.2 1,630,143 + -3.0 1,630,193 + -3.3 1,630,222 + -4.1 1,630,222 + -2.5 1,630,223 - -3.4 1,630,226 + -2.7 1,630,226 + -1.2 1,630,244 - -3.3 1,630,260 + -2.5 1,630,358 + thrB BNILDI_08165 0.11 -2.2 1,630,358 + thrB BNILDI_08165 0.11 -2.3 1,630,358 + thrB BNILDI_08165 0.11 +0.2 1,630,358 + thrB BNILDI_08165 0.11 -2.0 1,630,359 - thrB BNILDI_08165 0.11 -5.9 1,630,359 - thrB BNILDI_08165 0.11 -4.5 1,630,359 - thrB BNILDI_08165 0.11 -3.5 1,630,443 + thrB BNILDI_08165 0.20 -3.4 1,630,444 - thrB BNILDI_08165 0.20 -3.0 1,630,522 - thrB BNILDI_08165 0.29 -3.9 1,630,542 - thrB BNILDI_08165 0.31 -4.0 1,630,821 + thrB BNILDI_08165 0.61 -1.1 1,630,897 + thrB BNILDI_08165 0.69 -2.4 1,630,941 + thrB BNILDI_08165 0.74 -2.7 1,630,954 + thrB BNILDI_08165 0.75 -3.9 1,631,061 - thrB BNILDI_08165 0.86 -1.3 1,631,114 - -3.2 1,631,143 - -3.2 1,631,197 + -3.9
Or see this region's nucleotide sequence