Strain Fitness in Escherichia coli ECRC62 around BNILDI_08160

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlasT and BNILDI_08155 are separated by 213 nucleotidesBNILDI_08155 and thrA are separated by 80 nucleotidesthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotides BNILDI_08150: lasT - tRNA/rRNA methyltransferase, at 1,626,745 to 1,627,431 lasT BNILDI_08155: BNILDI_08155 - thr operon leader peptide, at 1,627,645 to 1,627,710 _08155 BNILDI_08160: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 1,627,791 to 1,630,253 thrA BNILDI_08165: thrB - homoserine kinase, at 1,630,255 to 1,631,187 thrB BNILDI_08170: thrC - threonine synthase, at 1,631,188 to 1,632,474 thrC Position (kb) 1627 1628 1629 1630 1631Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 1626.828 kb on + strand, within lasTat 1626.997 kb on + strand, within lasTat 1627.003 kb on + strand, within lasTat 1627.004 kb on - strand, within lasTat 1627.004 kb on - strand, within lasTat 1627.116 kb on - strand, within lasTat 1627.134 kb on + strand, within lasTat 1627.135 kb on - strand, within lasTat 1627.325 kb on + strand, within lasTat 1627.325 kb on + strand, within lasTat 1627.325 kb on + strand, within lasTat 1627.325 kb on + strand, within lasTat 1627.326 kb on - strand, within lasTat 1627.326 kb on - strand, within lasTat 1627.364 kb on + strandat 1627.365 kb on - strandat 1627.444 kb on + strandat 1627.445 kb on - strandat 1627.484 kb on + strandat 1627.484 kb on + strandat 1627.485 kb on - strandat 1627.519 kb on + strandat 1627.551 kb on + strandat 1627.552 kb on - strandat 1627.620 kb on - strandat 1627.668 kb on + strand, within BNILDI_08155at 1627.668 kb on + strand, within BNILDI_08155at 1627.783 kb on + strandat 1627.784 kb on - strandat 1627.784 kb on - strandat 1628.092 kb on + strand, within thrAat 1628.093 kb on - strand, within thrAat 1628.093 kb on - strand, within thrAat 1628.093 kb on - strand, within thrAat 1628.114 kb on + strand, within thrAat 1628.114 kb on + strand, within thrAat 1628.115 kb on - strand, within thrAat 1628.115 kb on - strand, within thrAat 1628.115 kb on - strand, within thrAat 1628.115 kb on - strand, within thrAat 1628.115 kb on - strand, within thrAat 1628.268 kb on + strand, within thrAat 1628.269 kb on - strand, within thrAat 1628.487 kb on + strand, within thrAat 1628.487 kb on + strand, within thrAat 1628.488 kb on - strand, within thrAat 1628.489 kb on + strand, within thrAat 1628.666 kb on + strand, within thrAat 1628.729 kb on + strand, within thrAat 1628.856 kb on + strand, within thrAat 1628.861 kb on + strand, within thrAat 1628.925 kb on + strand, within thrAat 1628.995 kb on + strand, within thrAat 1628.996 kb on - strand, within thrAat 1628.996 kb on - strand, within thrAat 1629.006 kb on - strand, within thrAat 1629.062 kb on + strand, within thrAat 1629.122 kb on + strand, within thrAat 1629.270 kb on - strand, within thrAat 1629.283 kb on + strand, within thrAat 1629.284 kb on - strand, within thrAat 1629.284 kb on - strand, within thrAat 1629.421 kb on - strand, within thrAat 1629.481 kb on - strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.552 kb on + strand, within thrAat 1629.553 kb on - strand, within thrAat 1629.555 kb on + strand, within thrAat 1629.556 kb on - strand, within thrAat 1629.715 kb on - strand, within thrAat 1629.715 kb on - strand, within thrAat 1629.716 kb on + strand, within thrAat 1629.716 kb on + strand, within thrAat 1629.717 kb on - strand, within thrAat 1629.717 kb on - strand, within thrAat 1629.730 kb on + strand, within thrAat 1630.035 kb on - strandat 1630.082 kb on + strandat 1630.131 kb on + strandat 1630.132 kb on - strandat 1630.141 kb on - strandat 1630.143 kb on + strandat 1630.193 kb on + strandat 1630.222 kb on + strandat 1630.222 kb on + strandat 1630.223 kb on - strandat 1630.226 kb on + strandat 1630.226 kb on + strandat 1630.244 kb on - strandat 1630.260 kb on + strandat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.358 kb on + strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.359 kb on - strand, within thrBat 1630.443 kb on + strand, within thrBat 1630.444 kb on - strand, within thrBat 1630.522 kb on - strand, within thrBat 1630.542 kb on - strand, within thrBat 1630.821 kb on + strand, within thrBat 1630.897 kb on + strand, within thrBat 1630.941 kb on + strand, within thrBat 1630.954 kb on + strand, within thrBat 1631.061 kb on - strand, within thrBat 1631.114 kb on - strandat 1631.143 kb on - strandat 1631.197 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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1,626,828 + lasT BNILDI_08150 0.12 -0.3
1,626,997 + lasT BNILDI_08150 0.37 -1.3
1,627,003 + lasT BNILDI_08150 0.38 +0.5
1,627,004 - lasT BNILDI_08150 0.38 -0.3
1,627,004 - lasT BNILDI_08150 0.38 -0.0
1,627,116 - lasT BNILDI_08150 0.54 +0.4
1,627,134 + lasT BNILDI_08150 0.57 +0.0
1,627,135 - lasT BNILDI_08150 0.57 -1.4
1,627,325 + lasT BNILDI_08150 0.84 -0.3
1,627,325 + lasT BNILDI_08150 0.84 -0.6
1,627,325 + lasT BNILDI_08150 0.84 +1.9
1,627,325 + lasT BNILDI_08150 0.84 -0.1
1,627,326 - lasT BNILDI_08150 0.85 +0.9
1,627,326 - lasT BNILDI_08150 0.85 +0.6
1,627,364 + -1.2
1,627,365 - +1.4
1,627,444 + -0.6
1,627,445 - -0.1
1,627,484 + +0.0
1,627,484 + +0.5
1,627,485 - +0.8
1,627,519 + -0.1
1,627,551 + +0.7
1,627,552 - +0.4
1,627,620 - -3.6
1,627,668 + BNILDI_08155 0.35 +0.5
1,627,668 + BNILDI_08155 0.35 -1.2
1,627,783 + -2.0
1,627,784 - -2.5
1,627,784 - -2.7
1,628,092 + thrA BNILDI_08160 0.12 -2.8
1,628,093 - thrA BNILDI_08160 0.12 -1.4
1,628,093 - thrA BNILDI_08160 0.12 -3.3
1,628,093 - thrA BNILDI_08160 0.12 -1.4
1,628,114 + thrA BNILDI_08160 0.13 -2.5
1,628,114 + thrA BNILDI_08160 0.13 -1.4
1,628,115 - thrA BNILDI_08160 0.13 -2.1
1,628,115 - thrA BNILDI_08160 0.13 -4.3
1,628,115 - thrA BNILDI_08160 0.13 -3.9
1,628,115 - thrA BNILDI_08160 0.13 -3.8
1,628,115 - thrA BNILDI_08160 0.13 -3.3
1,628,268 + thrA BNILDI_08160 0.19 -2.3
1,628,269 - thrA BNILDI_08160 0.19 -0.5
1,628,487 + thrA BNILDI_08160 0.28 -2.0
1,628,487 + thrA BNILDI_08160 0.28 -3.0
1,628,488 - thrA BNILDI_08160 0.28 -2.0
1,628,489 + thrA BNILDI_08160 0.28 -4.5
1,628,666 + thrA BNILDI_08160 0.36 -1.0
1,628,729 + thrA BNILDI_08160 0.38 -2.3
1,628,856 + thrA BNILDI_08160 0.43 -2.7
1,628,861 + thrA BNILDI_08160 0.43 -2.8
1,628,925 + thrA BNILDI_08160 0.46 -3.5
1,628,995 + thrA BNILDI_08160 0.49 -3.3
1,628,996 - thrA BNILDI_08160 0.49 -1.6
1,628,996 - thrA BNILDI_08160 0.49 -1.7
1,629,006 - thrA BNILDI_08160 0.49 -1.5
1,629,062 + thrA BNILDI_08160 0.52 -1.6
1,629,122 + thrA BNILDI_08160 0.54 -1.2
1,629,270 - thrA BNILDI_08160 0.60 -3.2
1,629,283 + thrA BNILDI_08160 0.61 -1.6
1,629,284 - thrA BNILDI_08160 0.61 -3.3
1,629,284 - thrA BNILDI_08160 0.61 -2.8
1,629,421 - thrA BNILDI_08160 0.66 -0.8
1,629,481 - thrA BNILDI_08160 0.69 -3.5
1,629,552 + thrA BNILDI_08160 0.71 -2.6
1,629,552 + thrA BNILDI_08160 0.71 -3.5
1,629,552 + thrA BNILDI_08160 0.71 -2.7
1,629,553 - thrA BNILDI_08160 0.72 -2.9
1,629,555 + thrA BNILDI_08160 0.72 -3.9
1,629,556 - thrA BNILDI_08160 0.72 -4.3
1,629,715 - thrA BNILDI_08160 0.78 -3.1
1,629,715 - thrA BNILDI_08160 0.78 -2.5
1,629,716 + thrA BNILDI_08160 0.78 -2.5
1,629,716 + thrA BNILDI_08160 0.78 -2.8
1,629,717 - thrA BNILDI_08160 0.78 -3.2
1,629,717 - thrA BNILDI_08160 0.78 -6.0
1,629,730 + thrA BNILDI_08160 0.79 -2.8
1,630,035 - -2.4
1,630,082 + -2.1
1,630,131 + -5.5
1,630,132 - -2.1
1,630,141 - -2.2
1,630,143 + -3.0
1,630,193 + -3.3
1,630,222 + -4.1
1,630,222 + -2.5
1,630,223 - -3.4
1,630,226 + -2.7
1,630,226 + -1.2
1,630,244 - -3.3
1,630,260 + -2.5
1,630,358 + thrB BNILDI_08165 0.11 -2.2
1,630,358 + thrB BNILDI_08165 0.11 -2.3
1,630,358 + thrB BNILDI_08165 0.11 +0.2
1,630,358 + thrB BNILDI_08165 0.11 -2.0
1,630,359 - thrB BNILDI_08165 0.11 -5.9
1,630,359 - thrB BNILDI_08165 0.11 -4.5
1,630,359 - thrB BNILDI_08165 0.11 -3.5
1,630,443 + thrB BNILDI_08165 0.20 -3.4
1,630,444 - thrB BNILDI_08165 0.20 -3.0
1,630,522 - thrB BNILDI_08165 0.29 -3.9
1,630,542 - thrB BNILDI_08165 0.31 -4.0
1,630,821 + thrB BNILDI_08165 0.61 -1.1
1,630,897 + thrB BNILDI_08165 0.69 -2.4
1,630,941 + thrB BNILDI_08165 0.74 -2.7
1,630,954 + thrB BNILDI_08165 0.75 -3.9
1,631,061 - thrB BNILDI_08165 0.86 -1.3
1,631,114 - -3.2
1,631,143 - -3.2
1,631,197 + -3.9

Or see this region's nucleotide sequence