Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and miaA overlap by 8 nucleotides miaA and hfq are separated by 85 nucleotides hfq and hflX are separated by 76 nucleotides
BNILDI_06960: mutL - DNA mismatch repair endonuclease MutL, at 1,392,154 to 1,394,001
mutL
BNILDI_06965: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 1,393,994 to 1,394,944
miaA
BNILDI_06970: hfq - RNA chaperone Hfq, at 1,395,030 to 1,395,338
hfq
BNILDI_06975: hflX - GTPase HflX, at 1,395,415 to 1,396,695
hflX
Position (kb)
1393
1394
1395 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1393.024 kb on + strand, within mutL at 1393.025 kb on - strand, within mutL at 1393.025 kb on - strand, within mutL at 1393.269 kb on - strand, within mutL at 1393.280 kb on - strand, within mutL at 1393.280 kb on - strand, within mutL at 1393.343 kb on - strand, within mutL at 1393.967 kb on + strand at 1394.100 kb on + strand, within miaA at 1394.128 kb on + strand, within miaA at 1394.132 kb on + strand, within miaA at 1394.576 kb on + strand, within miaA at 1394.913 kb on + strand at 1394.914 kb on - strand at 1394.950 kb on + strand at 1395.007 kb on + strand at 1395.007 kb on + strand at 1395.013 kb on + strand at 1395.276 kb on + strand, within hfq at 1395.276 kb on + strand, within hfq at 1395.276 kb on + strand, within hfq at 1395.276 kb on + strand, within hfq at 1395.276 kb on + strand, within hfq at 1395.410 kb on + strand at 1395.456 kb on - strand at 1395.463 kb on + strand at 1395.463 kb on + strand at 1395.463 kb on + strand at 1395.464 kb on - strand at 1395.587 kb on + strand, within hflX at 1395.597 kb on + strand, within hflX at 1395.733 kb on - strand, within hflX at 1395.742 kb on - strand, within hflX at 1395.863 kb on + strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,393,024 + mutL BNILDI_06960 0.47 +0.0 1,393,025 - mutL BNILDI_06960 0.47 -0.4 1,393,025 - mutL BNILDI_06960 0.47 +0.2 1,393,269 - mutL BNILDI_06960 0.60 +0.0 1,393,280 - mutL BNILDI_06960 0.61 +0.8 1,393,280 - mutL BNILDI_06960 0.61 -0.1 1,393,343 - mutL BNILDI_06960 0.64 -0.4 1,393,967 + -1.3 1,394,100 + miaA BNILDI_06965 0.11 -1.4 1,394,128 + miaA BNILDI_06965 0.14 -1.1 1,394,132 + miaA BNILDI_06965 0.15 -2.2 1,394,576 + miaA BNILDI_06965 0.61 -1.6 1,394,913 + -1.0 1,394,914 - -1.7 1,394,950 + -0.2 1,395,007 + -0.2 1,395,007 + +0.1 1,395,013 + +0.4 1,395,276 + hfq BNILDI_06970 0.80 +0.1 1,395,276 + hfq BNILDI_06970 0.80 +0.8 1,395,276 + hfq BNILDI_06970 0.80 +0.2 1,395,276 + hfq BNILDI_06970 0.80 +0.1 1,395,276 + hfq BNILDI_06970 0.80 +0.2 1,395,410 + +2.1 1,395,456 - +1.0 1,395,463 + +1.6 1,395,463 + +0.6 1,395,463 + -2.2 1,395,464 - -0.6 1,395,587 + hflX BNILDI_06975 0.13 +1.0 1,395,597 + hflX BNILDI_06975 0.14 -0.0 1,395,733 - hflX BNILDI_06975 0.25 -1.1 1,395,742 - hflX BNILDI_06975 0.26 +0.4 1,395,863 + hflX BNILDI_06975 0.35 +1.9
Or see this region's nucleotide sequence