Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiF and thiE overlap by 8 nucleotides thiE and thiC overlap by 1 nucleotides thiC and rsd are separated by 232 nucleotides rsd and nudC are separated by 94 nucleotides
BNILDI_05995: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,192,456 to 1,193,211
thiF
BNILDI_06000: thiE - thiamine phosphate synthase, at 1,193,204 to 1,193,839
thiE
BNILDI_06005: thiC - phosphomethylpyrimidine synthase ThiC, at 1,193,839 to 1,195,734
thiC
BNILDI_06010: rsd - sigma D regulator, at 1,195,967 to 1,196,443
rsd
BNILDI_06015: nudC - NAD(+) diphosphatase, at 1,196,538 to 1,197,311
nudC
Position (kb)
1193
1194
1195
1196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1192.903 kb on + strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.076 kb on - strand, within thiF at 1193.184 kb on + strand at 1193.464 kb on + strand, within thiE at 1193.465 kb on - strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.598 kb on + strand, within thiE at 1193.599 kb on - strand, within thiE at 1193.599 kb on - strand, within thiE at 1193.638 kb on + strand, within thiE at 1193.727 kb on - strand, within thiE at 1193.737 kb on + strand, within thiE at 1193.738 kb on - strand, within thiE at 1193.738 kb on - strand, within thiE at 1193.741 kb on + strand, within thiE at 1193.742 kb on - strand, within thiE at 1193.774 kb on + strand, within thiE at 1193.775 kb on - strand, within thiE at 1193.775 kb on - strand, within thiE at 1193.835 kb on + strand at 1193.836 kb on - strand at 1193.920 kb on - strand at 1193.920 kb on - strand at 1193.920 kb on - strand at 1194.075 kb on + strand, within thiC at 1194.183 kb on + strand, within thiC at 1194.183 kb on + strand, within thiC at 1194.185 kb on + strand, within thiC at 1194.185 kb on + strand, within thiC at 1194.185 kb on + strand, within thiC at 1194.186 kb on - strand, within thiC at 1194.193 kb on - strand, within thiC at 1194.205 kb on - strand, within thiC at 1194.216 kb on + strand, within thiC at 1194.217 kb on - strand, within thiC at 1194.220 kb on - strand, within thiC at 1194.251 kb on + strand, within thiC at 1194.318 kb on - strand, within thiC at 1194.337 kb on - strand, within thiC at 1194.382 kb on + strand, within thiC at 1194.382 kb on + strand, within thiC at 1194.459 kb on - strand, within thiC at 1194.493 kb on - strand, within thiC at 1194.493 kb on - strand, within thiC at 1194.534 kb on + strand, within thiC at 1194.535 kb on - strand, within thiC at 1194.535 kb on - strand, within thiC at 1194.579 kb on - strand, within thiC at 1194.716 kb on + strand, within thiC at 1194.716 kb on + strand, within thiC at 1194.727 kb on + strand, within thiC at 1194.727 kb on + strand, within thiC at 1194.750 kb on - strand, within thiC at 1194.805 kb on - strand, within thiC at 1194.845 kb on + strand, within thiC at 1194.858 kb on + strand, within thiC at 1194.909 kb on + strand, within thiC at 1194.910 kb on - strand, within thiC at 1194.910 kb on - strand, within thiC at 1194.910 kb on - strand, within thiC at 1194.965 kb on - strand, within thiC at 1195.081 kb on - strand, within thiC at 1195.192 kb on + strand, within thiC at 1195.328 kb on - strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.330 kb on + strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.331 kb on - strand, within thiC at 1195.632 kb on + strand at 1195.634 kb on + strand at 1195.634 kb on + strand at 1195.635 kb on - strand at 1195.681 kb on - strand at 1195.734 kb on + strand at 1195.735 kb on - strand at 1195.735 kb on - strand at 1195.735 kb on - strand at 1195.822 kb on + strand at 1195.823 kb on - strand at 1195.823 kb on - strand at 1195.844 kb on + strand at 1195.844 kb on + strand at 1195.895 kb on - strand at 1196.209 kb on + strand, within rsd at 1196.226 kb on + strand, within rsd at 1196.253 kb on + strand, within rsd at 1196.253 kb on + strand, within rsd at 1196.254 kb on - strand, within rsd at 1196.254 kb on - strand, within rsd at 1196.307 kb on + strand, within rsd at 1196.308 kb on - strand, within rsd at 1196.346 kb on + strand, within rsd at 1196.347 kb on - strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.365 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.369 kb on + strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.370 kb on - strand, within rsd at 1196.375 kb on + strand, within rsd at 1196.398 kb on + strand at 1196.399 kb on - strand at 1196.526 kb on - strand at 1196.537 kb on + strand at 1196.546 kb on + strand at 1196.549 kb on - strand at 1196.599 kb on + strand at 1196.707 kb on + strand, within nudC at 1196.707 kb on + strand, within nudC at 1196.708 kb on - strand, within nudC
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,192,903 + thiF BNILDI_05995 0.59 -0.1 1,192,904 - thiF BNILDI_05995 0.59 -0.3 1,192,904 - thiF BNILDI_05995 0.59 -3.1 1,192,904 - thiF BNILDI_05995 0.59 -0.1 1,192,904 - thiF BNILDI_05995 0.59 -0.6 1,193,075 + thiF BNILDI_05995 0.82 -1.5 1,193,075 + thiF BNILDI_05995 0.82 -1.0 1,193,075 + thiF BNILDI_05995 0.82 -0.8 1,193,076 - thiF BNILDI_05995 0.82 +0.7 1,193,184 + -1.3 1,193,464 + thiE BNILDI_06000 0.41 +0.3 1,193,465 - thiE BNILDI_06000 0.41 +0.1 1,193,466 + thiE BNILDI_06000 0.41 +0.5 1,193,466 + thiE BNILDI_06000 0.41 +2.4 1,193,466 + thiE BNILDI_06000 0.41 -0.6 1,193,467 - thiE BNILDI_06000 0.41 -0.3 1,193,467 - thiE BNILDI_06000 0.41 -2.6 1,193,467 - thiE BNILDI_06000 0.41 -0.4 1,193,467 - thiE BNILDI_06000 0.41 -0.8 1,193,598 + thiE BNILDI_06000 0.62 +0.2 1,193,599 - thiE BNILDI_06000 0.62 -0.9 1,193,599 - thiE BNILDI_06000 0.62 -0.8 1,193,638 + thiE BNILDI_06000 0.68 -0.3 1,193,727 - thiE BNILDI_06000 0.82 -1.3 1,193,737 + thiE BNILDI_06000 0.84 -0.2 1,193,738 - thiE BNILDI_06000 0.84 -2.3 1,193,738 - thiE BNILDI_06000 0.84 -0.2 1,193,741 + thiE BNILDI_06000 0.84 +0.1 1,193,742 - thiE BNILDI_06000 0.85 +1.2 1,193,774 + thiE BNILDI_06000 0.90 -0.8 1,193,775 - thiE BNILDI_06000 0.90 -1.1 1,193,775 - thiE BNILDI_06000 0.90 -0.8 1,193,835 + -0.9 1,193,836 - -3.2 1,193,920 - -1.2 1,193,920 - +0.6 1,193,920 - -1.6 1,194,075 + thiC BNILDI_06005 0.12 +0.1 1,194,183 + thiC BNILDI_06005 0.18 +1.3 1,194,183 + thiC BNILDI_06005 0.18 -1.8 1,194,185 + thiC BNILDI_06005 0.18 -0.6 1,194,185 + thiC BNILDI_06005 0.18 -0.3 1,194,185 + thiC BNILDI_06005 0.18 +1.5 1,194,186 - thiC BNILDI_06005 0.18 -1.0 1,194,193 - thiC BNILDI_06005 0.19 -0.3 1,194,205 - thiC BNILDI_06005 0.19 -1.2 1,194,216 + thiC BNILDI_06005 0.20 -1.6 1,194,217 - thiC BNILDI_06005 0.20 -0.8 1,194,220 - thiC BNILDI_06005 0.20 -0.5 1,194,251 + thiC BNILDI_06005 0.22 -0.1 1,194,318 - thiC BNILDI_06005 0.25 -0.6 1,194,337 - thiC BNILDI_06005 0.26 +0.1 1,194,382 + thiC BNILDI_06005 0.29 +0.5 1,194,382 + thiC BNILDI_06005 0.29 +1.5 1,194,459 - thiC BNILDI_06005 0.33 -1.5 1,194,493 - thiC BNILDI_06005 0.34 -0.5 1,194,493 - thiC BNILDI_06005 0.34 -0.3 1,194,534 + thiC BNILDI_06005 0.37 -1.5 1,194,535 - thiC BNILDI_06005 0.37 +0.2 1,194,535 - thiC BNILDI_06005 0.37 -1.1 1,194,579 - thiC BNILDI_06005 0.39 -1.4 1,194,716 + thiC BNILDI_06005 0.46 -0.4 1,194,716 + thiC BNILDI_06005 0.46 -1.4 1,194,727 + thiC BNILDI_06005 0.47 +0.1 1,194,727 + thiC BNILDI_06005 0.47 -1.3 1,194,750 - thiC BNILDI_06005 0.48 -3.7 1,194,805 - thiC BNILDI_06005 0.51 -0.9 1,194,845 + thiC BNILDI_06005 0.53 -1.2 1,194,858 + thiC BNILDI_06005 0.54 -1.1 1,194,909 + thiC BNILDI_06005 0.56 -1.0 1,194,910 - thiC BNILDI_06005 0.56 +0.7 1,194,910 - thiC BNILDI_06005 0.56 -0.5 1,194,910 - thiC BNILDI_06005 0.56 -0.6 1,194,965 - thiC BNILDI_06005 0.59 -1.8 1,195,081 - thiC BNILDI_06005 0.66 -2.8 1,195,192 + thiC BNILDI_06005 0.71 +0.1 1,195,328 - thiC BNILDI_06005 0.79 +0.2 1,195,330 + thiC BNILDI_06005 0.79 -0.7 1,195,330 + thiC BNILDI_06005 0.79 -2.4 1,195,330 + thiC BNILDI_06005 0.79 -0.5 1,195,331 - thiC BNILDI_06005 0.79 -0.4 1,195,331 - thiC BNILDI_06005 0.79 +0.6 1,195,331 - thiC BNILDI_06005 0.79 +0.5 1,195,331 - thiC BNILDI_06005 0.79 -0.5 1,195,632 + -0.6 1,195,634 + -1.8 1,195,634 + -2.0 1,195,635 - -1.6 1,195,681 - -0.3 1,195,734 + +0.2 1,195,735 - -0.1 1,195,735 - -0.7 1,195,735 - -1.0 1,195,822 + -0.9 1,195,823 - -1.1 1,195,823 - -0.9 1,195,844 + +0.1 1,195,844 + -1.4 1,195,895 - -0.3 1,196,209 + rsd BNILDI_06010 0.51 +1.0 1,196,226 + rsd BNILDI_06010 0.54 -0.3 1,196,253 + rsd BNILDI_06010 0.60 +0.4 1,196,253 + rsd BNILDI_06010 0.60 +0.2 1,196,254 - rsd BNILDI_06010 0.60 +0.3 1,196,254 - rsd BNILDI_06010 0.60 +0.1 1,196,307 + rsd BNILDI_06010 0.71 -0.0 1,196,308 - rsd BNILDI_06010 0.71 +0.1 1,196,346 + rsd BNILDI_06010 0.79 +1.4 1,196,347 - rsd BNILDI_06010 0.80 -0.8 1,196,365 + rsd BNILDI_06010 0.83 +0.5 1,196,365 + rsd BNILDI_06010 0.83 +1.4 1,196,365 + rsd BNILDI_06010 0.83 +1.0 1,196,369 + rsd BNILDI_06010 0.84 +1.0 1,196,369 + rsd BNILDI_06010 0.84 -2.2 1,196,369 + rsd BNILDI_06010 0.84 -1.9 1,196,369 + rsd BNILDI_06010 0.84 +0.0 1,196,370 - rsd BNILDI_06010 0.84 +1.0 1,196,370 - rsd BNILDI_06010 0.84 +0.1 1,196,370 - rsd BNILDI_06010 0.84 -0.2 1,196,370 - rsd BNILDI_06010 0.84 +1.0 1,196,375 + rsd BNILDI_06010 0.86 +0.2 1,196,398 + -0.4 1,196,399 - -0.4 1,196,526 - +0.3 1,196,537 + +0.5 1,196,546 + +0.2 1,196,549 - -0.1 1,196,599 + -1.7 1,196,707 + nudC BNILDI_06015 0.22 +1.1 1,196,707 + nudC BNILDI_06015 0.22 -2.6 1,196,708 - nudC BNILDI_06015 0.22 +1.5
Or see this region's nucleotide sequence