Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiF are separated by 1 nucleotides thiF and thiF overlap by 17 nucleotides thiF and thiE overlap by 8 nucleotides
BNILDI_05980: thiH - 2-iminoacetate synthase ThiH, at 1,190,370 to 1,191,503
thiH
BNILDI_05985: thiG - thiazole synthase, at 1,191,500 to 1,192,270
thiG
BNILDI_05990: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,192,272 to 1,192,472
thiF
BNILDI_05995: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,192,456 to 1,193,211
thiF
BNILDI_06000: thiE - thiamine phosphate synthase, at 1,193,204 to 1,193,839
thiE
Position (kb) 1192
1193 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1191.277 kb on + strand, within thiH at 1191.278 kb on - strand, within thiH at 1191.284 kb on + strand, within thiH at 1191.284 kb on + strand, within thiH at 1191.284 kb on + strand, within thiH at 1191.380 kb on + strand, within thiH at 1191.408 kb on + strand at 1191.420 kb on + strand at 1191.447 kb on - strand at 1191.763 kb on + strand, within thiG at 1191.763 kb on + strand, within thiG at 1191.859 kb on + strand, within thiG at 1191.885 kb on + strand, within thiG at 1192.066 kb on + strand, within thiG at 1192.067 kb on - strand, within thiG at 1192.262 kb on - strand at 1192.325 kb on + strand, within thiF at 1192.326 kb on - strand, within thiF at 1192.365 kb on - strand, within thiF at 1192.411 kb on - strand, within thiF at 1192.411 kb on - strand, within thiF at 1192.576 kb on + strand, within thiF at 1192.579 kb on - strand, within thiF at 1192.588 kb on + strand, within thiF at 1192.589 kb on - strand, within thiF at 1192.594 kb on - strand, within thiF at 1192.704 kb on - strand, within thiF at 1192.774 kb on - strand, within thiF at 1192.807 kb on - strand, within thiF at 1192.827 kb on + strand, within thiF at 1192.828 kb on - strand, within thiF at 1192.903 kb on + strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1192.904 kb on - strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.075 kb on + strand, within thiF at 1193.076 kb on - strand, within thiF at 1193.184 kb on + strand at 1193.464 kb on + strand, within thiE at 1193.465 kb on - strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.466 kb on + strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE at 1193.467 kb on - strand, within thiE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,191,277 + thiH BNILDI_05980 0.80 -1.6 1,191,278 - thiH BNILDI_05980 0.80 -0.2 1,191,284 + thiH BNILDI_05980 0.81 -3.9 1,191,284 + thiH BNILDI_05980 0.81 -0.3 1,191,284 + thiH BNILDI_05980 0.81 +0.9 1,191,380 + thiH BNILDI_05980 0.89 -0.1 1,191,408 + -2.0 1,191,420 + -0.1 1,191,447 - -1.6 1,191,763 + thiG BNILDI_05985 0.34 +0.2 1,191,763 + thiG BNILDI_05985 0.34 -0.6 1,191,859 + thiG BNILDI_05985 0.47 -1.3 1,191,885 + thiG BNILDI_05985 0.50 +0.6 1,192,066 + thiG BNILDI_05985 0.73 +0.5 1,192,067 - thiG BNILDI_05985 0.74 -0.1 1,192,262 - +0.7 1,192,325 + thiF BNILDI_05990 0.26 -0.5 1,192,326 - thiF BNILDI_05990 0.27 -0.7 1,192,365 - thiF BNILDI_05990 0.46 -1.3 1,192,411 - thiF BNILDI_05990 0.69 -0.9 1,192,411 - thiF BNILDI_05990 0.69 -0.9 1,192,576 + thiF BNILDI_05995 0.16 -0.2 1,192,579 - thiF BNILDI_05995 0.16 -0.8 1,192,588 + thiF BNILDI_05995 0.17 -0.9 1,192,589 - thiF BNILDI_05995 0.18 -0.3 1,192,594 - thiF BNILDI_05995 0.18 +0.5 1,192,704 - thiF BNILDI_05995 0.33 -1.6 1,192,774 - thiF BNILDI_05995 0.42 +1.6 1,192,807 - thiF BNILDI_05995 0.46 +0.3 1,192,827 + thiF BNILDI_05995 0.49 -0.9 1,192,828 - thiF BNILDI_05995 0.49 -1.2 1,192,903 + thiF BNILDI_05995 0.59 -0.1 1,192,904 - thiF BNILDI_05995 0.59 -0.3 1,192,904 - thiF BNILDI_05995 0.59 -3.1 1,192,904 - thiF BNILDI_05995 0.59 -0.1 1,192,904 - thiF BNILDI_05995 0.59 -0.6 1,193,075 + thiF BNILDI_05995 0.82 -1.5 1,193,075 + thiF BNILDI_05995 0.82 -1.0 1,193,075 + thiF BNILDI_05995 0.82 -0.8 1,193,076 - thiF BNILDI_05995 0.82 +0.7 1,193,184 + -1.3 1,193,464 + thiE BNILDI_06000 0.41 +0.3 1,193,465 - thiE BNILDI_06000 0.41 +0.1 1,193,466 + thiE BNILDI_06000 0.41 +0.5 1,193,466 + thiE BNILDI_06000 0.41 +2.4 1,193,466 + thiE BNILDI_06000 0.41 -0.6 1,193,467 - thiE BNILDI_06000 0.41 -0.3 1,193,467 - thiE BNILDI_06000 0.41 -2.6 1,193,467 - thiE BNILDI_06000 0.41 -0.4 1,193,467 - thiE BNILDI_06000 0.41 -0.8
Or see this region's nucleotide sequence