Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaA and Thr_trna are separated by 385 nucleotides Thr_trna and Tyr_trna are separated by 8 nucleotides Tyr_trna and Gly_trna are separated by 116 nucleotides Gly_trna and Thr_trna are separated by 6 nucleotides Thr_trna and tuf are separated by 114 nucleotides
BNILDI_05895: coaA - type I pantothenate kinase, at 1,173,711 to 1,174,637
coaA
BNILDI_05900: Thr_trna - tRNA-Thr(tgt), at 1,175,023 to 1,175,098
_trna
BNILDI_05905: Tyr_trna - tRNA-Tyr(gta), at 1,175,107 to 1,175,191
_trna
BNILDI_05910: Gly_trna - tRNA-Gly(tcc), at 1,175,308 to 1,175,382
_trna
BNILDI_05915: Thr_trna - tRNA-Thr(ggt), at 1,175,389 to 1,175,464
_trna
BNILDI_05920: tuf - elongation factor Tu, at 1,175,579 to 1,176,763
tuf
Position (kb)
1175
1176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1174.653 kb on + strand at 1174.653 kb on + strand at 1174.653 kb on + strand at 1174.654 kb on - strand at 1174.655 kb on + strand at 1174.662 kb on - strand at 1174.662 kb on - strand at 1174.682 kb on + strand at 1174.682 kb on + strand at 1174.682 kb on + strand at 1174.682 kb on + strand at 1174.683 kb on - strand at 1174.703 kb on - strand at 1174.740 kb on - strand at 1174.774 kb on + strand at 1174.774 kb on + strand at 1174.777 kb on + strand at 1174.803 kb on - strand at 1174.827 kb on + strand at 1174.827 kb on + strand at 1174.828 kb on - strand at 1174.849 kb on + strand at 1174.849 kb on + strand at 1174.850 kb on - strand at 1174.850 kb on - strand at 1174.857 kb on - strand at 1174.901 kb on + strand at 1175.142 kb on + strand, within Tyr_trna at 1175.395 kb on - strand at 1175.397 kb on - strand, within Thr_trna at 1175.397 kb on - strand, within Thr_trna at 1175.543 kb on + strand at 1175.566 kb on - strand at 1175.585 kb on - strand at 1175.603 kb on - strand at 1175.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,174,653 + +2.9 1,174,653 + -1.3 1,174,653 + -3.5 1,174,654 - +0.6 1,174,655 + +0.8 1,174,662 - +0.1 1,174,662 - -2.1 1,174,682 + -0.5 1,174,682 + -0.0 1,174,682 + +0.5 1,174,682 + +0.3 1,174,683 - -2.1 1,174,703 - +1.0 1,174,740 - +0.2 1,174,774 + +0.2 1,174,774 + -0.3 1,174,777 + -0.8 1,174,803 - -0.5 1,174,827 + -0.2 1,174,827 + -0.4 1,174,828 - +0.9 1,174,849 + +0.5 1,174,849 + +0.4 1,174,850 - -0.2 1,174,850 - +0.2 1,174,857 - -0.3 1,174,901 + -1.1 1,175,142 + Tyr_trna BNILDI_05905 0.41 -2.7 1,175,395 - -2.4 1,175,397 - Thr_trna BNILDI_05915 0.11 +0.2 1,175,397 - Thr_trna BNILDI_05915 0.11 -1.3 1,175,543 + -1.2 1,175,566 - -0.4 1,175,585 - -0.6 1,175,603 - -1.2 1,175,697 - +1.4
Or see this region's nucleotide sequence