Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepQ and yigZ overlap by 1 nucleotides yigZ and trkH are separated by 38 nucleotides trkH and hemG are separated by 11 nucleotides hemG and 16S_rrna are separated by 375 nucleotides
BNILDI_05160: pepQ - Xaa-Pro dipeptidase, at 1,022,984 to 1,024,315
pepQ
BNILDI_05165: yigZ - IMPACT family protein, at 1,024,315 to 1,024,929
yigZ
BNILDI_05170: trkH - Trk system potassium transporter TrkH, at 1,024,968 to 1,026,419
trkH
BNILDI_05175: hemG - menaquinone-dependent protoporphyrinogen IX dehydrogenase, at 1,026,431 to 1,026,976
hemG
BNILDI_05180: 16S_rrna - 16S ribosomal RNA, at 1,027,352 to 1,028,893
_rrna
Position (kb) 1024
1025
1026
1027 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1023.980 kb on - strand, within pepQ at 1024.027 kb on - strand, within pepQ at 1024.145 kb on + strand, within pepQ at 1024.145 kb on + strand, within pepQ at 1024.145 kb on + strand, within pepQ at 1024.145 kb on + strand, within pepQ at 1024.145 kb on + strand, within pepQ at 1024.146 kb on - strand, within pepQ at 1024.146 kb on - strand, within pepQ at 1024.146 kb on - strand, within pepQ at 1024.146 kb on - strand, within pepQ at 1024.329 kb on - strand at 1024.555 kb on - strand, within yigZ at 1024.622 kb on - strand, within yigZ at 1024.731 kb on + strand, within yigZ at 1024.731 kb on + strand, within yigZ at 1024.732 kb on - strand, within yigZ at 1024.747 kb on + strand, within yigZ at 1024.748 kb on - strand, within yigZ at 1024.749 kb on + strand, within yigZ at 1024.952 kb on + strand at 1024.952 kb on + strand at 1024.952 kb on + strand at 1025.084 kb on + strand at 1025.679 kb on - strand, within trkH at 1025.681 kb on + strand, within trkH at 1025.682 kb on - strand, within trkH at 1025.697 kb on - strand, within trkH at 1025.725 kb on - strand, within trkH at 1025.746 kb on + strand, within trkH at 1025.747 kb on - strand, within trkH at 1025.813 kb on + strand, within trkH at 1025.832 kb on - strand, within trkH at 1025.860 kb on - strand, within trkH at 1025.882 kb on + strand, within trkH at 1026.056 kb on + strand, within trkH at 1026.105 kb on + strand, within trkH at 1026.113 kb on + strand, within trkH at 1026.315 kb on - strand at 1026.428 kb on + strand at 1026.428 kb on + strand at 1026.952 kb on - strand at 1026.974 kb on + strand at 1026.975 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 1,023,980 - pepQ BNILDI_05160 0.75 -0.1 1,024,027 - pepQ BNILDI_05160 0.78 -0.7 1,024,145 + pepQ BNILDI_05160 0.87 -0.3 1,024,145 + pepQ BNILDI_05160 0.87 -0.0 1,024,145 + pepQ BNILDI_05160 0.87 +0.0 1,024,145 + pepQ BNILDI_05160 0.87 -0.1 1,024,145 + pepQ BNILDI_05160 0.87 +0.7 1,024,146 - pepQ BNILDI_05160 0.87 -0.5 1,024,146 - pepQ BNILDI_05160 0.87 -1.0 1,024,146 - pepQ BNILDI_05160 0.87 -0.2 1,024,146 - pepQ BNILDI_05160 0.87 -0.4 1,024,329 - -1.0 1,024,555 - yigZ BNILDI_05165 0.39 +0.2 1,024,622 - yigZ BNILDI_05165 0.50 +0.6 1,024,731 + yigZ BNILDI_05165 0.68 -3.2 1,024,731 + yigZ BNILDI_05165 0.68 -0.1 1,024,732 - yigZ BNILDI_05165 0.68 +0.8 1,024,747 + yigZ BNILDI_05165 0.70 +0.2 1,024,748 - yigZ BNILDI_05165 0.70 -0.3 1,024,749 + yigZ BNILDI_05165 0.71 +0.8 1,024,952 + -0.3 1,024,952 + +0.4 1,024,952 + -0.1 1,025,084 + -2.8 1,025,679 - trkH BNILDI_05170 0.49 -0.9 1,025,681 + trkH BNILDI_05170 0.49 -0.9 1,025,682 - trkH BNILDI_05170 0.49 -0.6 1,025,697 - trkH BNILDI_05170 0.50 -1.2 1,025,725 - trkH BNILDI_05170 0.52 +2.5 1,025,746 + trkH BNILDI_05170 0.54 -0.8 1,025,747 - trkH BNILDI_05170 0.54 -1.1 1,025,813 + trkH BNILDI_05170 0.58 -1.0 1,025,832 - trkH BNILDI_05170 0.60 -3.5 1,025,860 - trkH BNILDI_05170 0.61 -0.2 1,025,882 + trkH BNILDI_05170 0.63 -0.4 1,026,056 + trkH BNILDI_05170 0.75 -0.5 1,026,105 + trkH BNILDI_05170 0.78 -0.1 1,026,113 + trkH BNILDI_05170 0.79 +0.1 1,026,315 - -0.5 1,026,428 + +1.6 1,026,428 + +0.0 1,026,952 - -0.3 1,026,974 + +0.8 1,026,975 - +2.0
Or see this region's nucleotide sequence