Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvG and BNILDI_04740 overlap by 4 nucleotides BNILDI_04740 and ilvE are separated by 19 nucleotides ilvE and ilvD are separated by 64 nucleotides
BNILDI_04735: ilvG - acetolactate synthase 2 catalytic subunit, at 937,542 to 939,188
ilvG
BNILDI_04740: BNILDI_04740 - Acetolactate synthase isozyme 2 small subunit, at 939,185 to 939,448
_04740
BNILDI_04745: ilvE - branched-chain-amino-acid transaminase, at 939,468 to 940,397
ilvE
BNILDI_04750: ilvD - dihydroxy-acid dehydratase, at 940,462 to 942,312
ilvD
Position (kb) 939
940
941 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 938.470 kb on + strand, within ilvG at 938.470 kb on + strand, within ilvG at 938.482 kb on + strand, within ilvG at 938.482 kb on + strand, within ilvG at 938.503 kb on + strand, within ilvG at 938.503 kb on + strand, within ilvG at 938.536 kb on + strand, within ilvG at 938.594 kb on + strand, within ilvG at 938.599 kb on - strand, within ilvG at 938.705 kb on + strand, within ilvG at 938.741 kb on - strand, within ilvG at 938.813 kb on + strand, within ilvG at 938.845 kb on + strand, within ilvG at 938.845 kb on + strand, within ilvG at 938.846 kb on - strand, within ilvG at 938.871 kb on - strand, within ilvG at 938.959 kb on - strand, within ilvG at 939.158 kb on + strand at 939.159 kb on - strand at 939.181 kb on + strand at 939.181 kb on + strand at 939.234 kb on + strand, within BNILDI_04740 at 939.234 kb on + strand, within BNILDI_04740 at 939.234 kb on + strand, within BNILDI_04740 at 939.247 kb on - strand, within BNILDI_04740 at 939.290 kb on - strand, within BNILDI_04740 at 939.318 kb on + strand, within BNILDI_04740 at 939.319 kb on - strand, within BNILDI_04740 at 939.319 kb on - strand, within BNILDI_04740 at 939.361 kb on - strand, within BNILDI_04740 at 939.368 kb on - strand, within BNILDI_04740 at 939.375 kb on + strand, within BNILDI_04740 at 939.375 kb on + strand, within BNILDI_04740 at 939.376 kb on - strand, within BNILDI_04740 at 939.379 kb on + strand, within BNILDI_04740 at 939.379 kb on + strand, within BNILDI_04740 at 939.380 kb on - strand, within BNILDI_04740 at 939.463 kb on + strand at 939.489 kb on + strand at 939.490 kb on - strand at 939.561 kb on + strand, within ilvE at 939.595 kb on - strand, within ilvE at 939.638 kb on + strand, within ilvE at 939.737 kb on + strand, within ilvE at 939.738 kb on - strand, within ilvE at 939.738 kb on - strand, within ilvE at 939.797 kb on - strand, within ilvE at 939.956 kb on + strand, within ilvE at 939.961 kb on - strand, within ilvE at 939.961 kb on - strand, within ilvE at 939.961 kb on - strand, within ilvE at 940.039 kb on - strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.041 kb on - strand, within ilvE at 940.041 kb on - strand, within ilvE at 940.177 kb on + strand, within ilvE at 940.178 kb on - strand, within ilvE at 940.229 kb on + strand, within ilvE at 940.229 kb on + strand, within ilvE at 940.229 kb on + strand, within ilvE at 940.230 kb on - strand, within ilvE at 940.230 kb on - strand, within ilvE at 940.364 kb on + strand at 940.378 kb on + strand at 940.378 kb on + strand at 940.379 kb on - strand at 940.379 kb on - strand at 940.389 kb on - strand at 940.389 kb on - strand at 940.395 kb on + strand at 940.395 kb on + strand at 940.453 kb on - strand at 940.453 kb on - strand at 940.456 kb on + strand at 940.456 kb on + strand at 940.461 kb on + strand at 940.461 kb on + strand at 940.462 kb on - strand at 940.467 kb on + strand at 940.467 kb on + strand at 940.467 kb on + strand at 940.468 kb on - strand at 940.468 kb on - strand at 940.468 kb on - strand at 940.500 kb on + strand at 940.500 kb on + strand at 940.501 kb on - strand at 940.504 kb on - strand at 940.601 kb on + strand at 940.777 kb on + strand, within ilvD at 940.777 kb on + strand, within ilvD at 940.777 kb on + strand, within ilvD at 940.777 kb on + strand, within ilvD at 940.778 kb on - strand, within ilvD at 940.778 kb on - strand, within ilvD at 940.778 kb on - strand, within ilvD at 940.778 kb on - strand, within ilvD at 940.778 kb on - strand, within ilvD at 940.779 kb on + strand, within ilvD at 940.779 kb on + strand, within ilvD at 940.821 kb on + strand, within ilvD at 940.873 kb on - strand, within ilvD at 941.059 kb on - strand, within ilvD at 941.146 kb on - strand, within ilvD at 941.197 kb on + strand, within ilvD at 941.307 kb on - strand, within ilvD at 941.397 kb on + strand, within ilvD
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 938,470 + ilvG BNILDI_04735 0.56 -3.2 938,470 + ilvG BNILDI_04735 0.56 -0.9 938,482 + ilvG BNILDI_04735 0.57 -0.9 938,482 + ilvG BNILDI_04735 0.57 -0.7 938,503 + ilvG BNILDI_04735 0.58 -1.6 938,503 + ilvG BNILDI_04735 0.58 -0.6 938,536 + ilvG BNILDI_04735 0.60 +0.1 938,594 + ilvG BNILDI_04735 0.64 -0.0 938,599 - ilvG BNILDI_04735 0.64 -2.9 938,705 + ilvG BNILDI_04735 0.71 -0.7 938,741 - ilvG BNILDI_04735 0.73 -1.8 938,813 + ilvG BNILDI_04735 0.77 -1.2 938,845 + ilvG BNILDI_04735 0.79 -0.2 938,845 + ilvG BNILDI_04735 0.79 -0.5 938,846 - ilvG BNILDI_04735 0.79 -4.8 938,871 - ilvG BNILDI_04735 0.81 -3.1 938,959 - ilvG BNILDI_04735 0.86 -3.4 939,158 + -0.2 939,159 - -4.1 939,181 + +1.0 939,181 + -0.3 939,234 + BNILDI_04740 0.19 -0.2 939,234 + BNILDI_04740 0.19 -0.0 939,234 + BNILDI_04740 0.19 -0.3 939,247 - BNILDI_04740 0.23 -1.0 939,290 - BNILDI_04740 0.40 -2.1 939,318 + BNILDI_04740 0.50 -1.2 939,319 - BNILDI_04740 0.51 -0.8 939,319 - BNILDI_04740 0.51 -0.6 939,361 - BNILDI_04740 0.67 -3.3 939,368 - BNILDI_04740 0.69 -0.7 939,375 + BNILDI_04740 0.72 -1.1 939,375 + BNILDI_04740 0.72 -0.0 939,376 - BNILDI_04740 0.72 -4.6 939,379 + BNILDI_04740 0.73 +0.1 939,379 + BNILDI_04740 0.73 -0.6 939,380 - BNILDI_04740 0.74 -2.6 939,463 + -2.6 939,489 + +1.0 939,490 - -2.9 939,561 + ilvE BNILDI_04745 0.10 -4.8 939,595 - ilvE BNILDI_04745 0.14 -4.7 939,638 + ilvE BNILDI_04745 0.18 -2.7 939,737 + ilvE BNILDI_04745 0.29 -2.6 939,738 - ilvE BNILDI_04745 0.29 -1.8 939,738 - ilvE BNILDI_04745 0.29 -5.5 939,797 - ilvE BNILDI_04745 0.35 -3.8 939,956 + ilvE BNILDI_04745 0.52 +1.6 939,961 - ilvE BNILDI_04745 0.53 -1.8 939,961 - ilvE BNILDI_04745 0.53 -1.4 939,961 - ilvE BNILDI_04745 0.53 -1.4 940,039 - ilvE BNILDI_04745 0.61 -4.0 940,040 + ilvE BNILDI_04745 0.62 -3.0 940,040 + ilvE BNILDI_04745 0.62 -2.1 940,040 + ilvE BNILDI_04745 0.62 -0.3 940,041 - ilvE BNILDI_04745 0.62 -2.2 940,041 - ilvE BNILDI_04745 0.62 -3.5 940,177 + ilvE BNILDI_04745 0.76 -3.7 940,178 - ilvE BNILDI_04745 0.76 -3.7 940,229 + ilvE BNILDI_04745 0.82 -3.3 940,229 + ilvE BNILDI_04745 0.82 -4.6 940,229 + ilvE BNILDI_04745 0.82 -3.6 940,230 - ilvE BNILDI_04745 0.82 -3.2 940,230 - ilvE BNILDI_04745 0.82 -3.5 940,364 + -4.1 940,378 + -2.2 940,378 + -2.3 940,379 - -2.2 940,379 - -3.5 940,389 - -4.2 940,389 - -3.3 940,395 + +0.0 940,395 + -0.1 940,453 - -3.6 940,453 - -2.6 940,456 + -4.5 940,456 + -1.4 940,461 + -2.7 940,461 + -3.9 940,462 - -3.6 940,467 + -2.8 940,467 + -3.3 940,467 + -2.6 940,468 - -1.7 940,468 - -2.5 940,468 - -2.6 940,500 + -2.4 940,500 + -1.0 940,501 - -0.9 940,504 - -3.7 940,601 + -4.4 940,777 + ilvD BNILDI_04750 0.17 -3.0 940,777 + ilvD BNILDI_04750 0.17 +0.1 940,777 + ilvD BNILDI_04750 0.17 -3.2 940,777 + ilvD BNILDI_04750 0.17 -2.6 940,778 - ilvD BNILDI_04750 0.17 -2.1 940,778 - ilvD BNILDI_04750 0.17 -2.0 940,778 - ilvD BNILDI_04750 0.17 +0.3 940,778 - ilvD BNILDI_04750 0.17 -2.8 940,778 - ilvD BNILDI_04750 0.17 -2.2 940,779 + ilvD BNILDI_04750 0.17 -1.0 940,779 + ilvD BNILDI_04750 0.17 -3.3 940,821 + ilvD BNILDI_04750 0.19 -1.8 940,873 - ilvD BNILDI_04750 0.22 -1.3 941,059 - ilvD BNILDI_04750 0.32 -4.3 941,146 - ilvD BNILDI_04750 0.37 -4.6 941,197 + ilvD BNILDI_04750 0.40 -3.9 941,307 - ilvD BNILDI_04750 0.46 -4.5 941,397 + ilvD BNILDI_04750 0.51 -1.3
Or see this region's nucleotide sequence