Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifB and ilvX are separated by 537 nucleotides ilvX and ilvG are separated by 2 nucleotides ilvG and BNILDI_04740 overlap by 4 nucleotides BNILDI_04740 and ilvE are separated by 19 nucleotides
BNILDI_04725: yifB - YifB family Mg chelatase-like AAA ATPase, at 935,431 to 936,951
yifB
BNILDI_04730: ilvX - Uncharacterized protein IlvX, at 937,489 to 937,539
ilvX
BNILDI_04735: ilvG - acetolactate synthase 2 catalytic subunit, at 937,542 to 939,188
ilvG
BNILDI_04740: BNILDI_04740 - Acetolactate synthase isozyme 2 small subunit, at 939,185 to 939,448
_04740
BNILDI_04745: ilvE - branched-chain-amino-acid transaminase, at 939,468 to 940,397
ilvE
Position (kb) 937
938
939
940 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 936.561 kb on + strand, within yifB at 936.561 kb on + strand, within yifB at 936.562 kb on - strand, within yifB at 936.565 kb on - strand, within yifB at 936.649 kb on + strand, within yifB at 936.650 kb on - strand, within yifB at 936.654 kb on + strand, within yifB at 936.703 kb on + strand, within yifB at 936.703 kb on + strand, within yifB at 936.704 kb on - strand, within yifB at 936.704 kb on - strand, within yifB at 936.704 kb on - strand, within yifB at 936.704 kb on - strand, within yifB at 936.779 kb on + strand, within yifB at 936.842 kb on - strand at 936.857 kb on - strand at 936.879 kb on + strand at 936.879 kb on + strand at 936.879 kb on + strand at 936.880 kb on - strand at 936.880 kb on - strand at 936.880 kb on - strand at 936.884 kb on - strand at 936.884 kb on - strand at 936.884 kb on - strand at 936.884 kb on - strand at 936.911 kb on - strand at 936.951 kb on + strand at 936.951 kb on + strand at 936.951 kb on + strand at 936.951 kb on + strand at 936.952 kb on - strand at 936.952 kb on - strand at 936.973 kb on + strand at 936.973 kb on + strand at 937.112 kb on + strand at 937.112 kb on + strand at 937.113 kb on - strand at 937.260 kb on - strand at 937.327 kb on + strand at 937.400 kb on + strand at 937.422 kb on + strand at 937.538 kb on - strand at 937.538 kb on - strand at 937.542 kb on - strand at 937.542 kb on - strand at 937.564 kb on + strand at 937.565 kb on - strand at 937.645 kb on - strand at 937.645 kb on - strand at 937.738 kb on + strand, within ilvG at 937.738 kb on + strand, within ilvG at 937.739 kb on - strand, within ilvG at 937.739 kb on - strand, within ilvG at 937.742 kb on + strand, within ilvG at 937.742 kb on + strand, within ilvG at 937.742 kb on + strand, within ilvG at 937.743 kb on - strand, within ilvG at 937.809 kb on - strand, within ilvG at 938.030 kb on - strand, within ilvG at 938.145 kb on + strand, within ilvG at 938.146 kb on - strand, within ilvG at 938.272 kb on - strand, within ilvG at 938.417 kb on + strand, within ilvG at 938.470 kb on + strand, within ilvG at 938.470 kb on + strand, within ilvG at 938.482 kb on + strand, within ilvG at 938.482 kb on + strand, within ilvG at 938.503 kb on + strand, within ilvG at 938.503 kb on + strand, within ilvG at 938.536 kb on + strand, within ilvG at 938.594 kb on + strand, within ilvG at 938.599 kb on - strand, within ilvG at 938.705 kb on + strand, within ilvG at 938.741 kb on - strand, within ilvG at 938.813 kb on + strand, within ilvG at 938.845 kb on + strand, within ilvG at 938.845 kb on + strand, within ilvG at 938.846 kb on - strand, within ilvG at 938.871 kb on - strand, within ilvG at 938.959 kb on - strand, within ilvG at 939.158 kb on + strand at 939.159 kb on - strand at 939.181 kb on + strand at 939.181 kb on + strand at 939.234 kb on + strand, within BNILDI_04740 at 939.234 kb on + strand, within BNILDI_04740 at 939.234 kb on + strand, within BNILDI_04740 at 939.247 kb on - strand, within BNILDI_04740 at 939.290 kb on - strand, within BNILDI_04740 at 939.318 kb on + strand, within BNILDI_04740 at 939.319 kb on - strand, within BNILDI_04740 at 939.319 kb on - strand, within BNILDI_04740 at 939.361 kb on - strand, within BNILDI_04740 at 939.368 kb on - strand, within BNILDI_04740 at 939.375 kb on + strand, within BNILDI_04740 at 939.375 kb on + strand, within BNILDI_04740 at 939.376 kb on - strand, within BNILDI_04740 at 939.379 kb on + strand, within BNILDI_04740 at 939.379 kb on + strand, within BNILDI_04740 at 939.380 kb on - strand, within BNILDI_04740 at 939.463 kb on + strand at 939.489 kb on + strand at 939.490 kb on - strand at 939.561 kb on + strand, within ilvE at 939.595 kb on - strand, within ilvE at 939.638 kb on + strand, within ilvE at 939.737 kb on + strand, within ilvE at 939.738 kb on - strand, within ilvE at 939.738 kb on - strand, within ilvE at 939.797 kb on - strand, within ilvE at 939.956 kb on + strand, within ilvE at 939.961 kb on - strand, within ilvE at 939.961 kb on - strand, within ilvE at 939.961 kb on - strand, within ilvE at 940.039 kb on - strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.040 kb on + strand, within ilvE at 940.041 kb on - strand, within ilvE at 940.041 kb on - strand, within ilvE at 940.177 kb on + strand, within ilvE at 940.178 kb on - strand, within ilvE
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 936,561 + yifB BNILDI_04725 0.74 -1.6 936,561 + yifB BNILDI_04725 0.74 +0.3 936,562 - yifB BNILDI_04725 0.74 +0.9 936,565 - yifB BNILDI_04725 0.75 -0.1 936,649 + yifB BNILDI_04725 0.80 +0.4 936,650 - yifB BNILDI_04725 0.80 -0.2 936,654 + yifB BNILDI_04725 0.80 +1.2 936,703 + yifB BNILDI_04725 0.84 -2.8 936,703 + yifB BNILDI_04725 0.84 -1.2 936,704 - yifB BNILDI_04725 0.84 -0.0 936,704 - yifB BNILDI_04725 0.84 +0.4 936,704 - yifB BNILDI_04725 0.84 -0.0 936,704 - yifB BNILDI_04725 0.84 -0.6 936,779 + yifB BNILDI_04725 0.89 +0.9 936,842 - -0.2 936,857 - -0.5 936,879 + -1.1 936,879 + -0.1 936,879 + +0.1 936,880 - -0.1 936,880 - -0.1 936,880 - +0.2 936,884 - -0.6 936,884 - -1.0 936,884 - +0.0 936,884 - +0.2 936,911 - -1.9 936,951 + +0.3 936,951 + -0.2 936,951 + +0.8 936,951 + -0.1 936,952 - -0.6 936,952 - -1.0 936,973 + -1.4 936,973 + -1.8 937,112 + -0.8 937,112 + -0.3 937,113 - +0.3 937,260 - -3.2 937,327 + +0.2 937,400 + -0.6 937,422 + +0.1 937,538 - -1.8 937,538 - -1.8 937,542 - -1.7 937,542 - -1.5 937,564 + -0.2 937,565 - -1.7 937,645 - -2.2 937,645 - -2.1 937,738 + ilvG BNILDI_04735 0.12 -1.8 937,738 + ilvG BNILDI_04735 0.12 -1.0 937,739 - ilvG BNILDI_04735 0.12 -1.5 937,739 - ilvG BNILDI_04735 0.12 -2.2 937,742 + ilvG BNILDI_04735 0.12 -1.1 937,742 + ilvG BNILDI_04735 0.12 -2.3 937,742 + ilvG BNILDI_04735 0.12 -0.9 937,743 - ilvG BNILDI_04735 0.12 -1.2 937,809 - ilvG BNILDI_04735 0.16 -2.5 938,030 - ilvG BNILDI_04735 0.30 -1.8 938,145 + ilvG BNILDI_04735 0.37 -1.4 938,146 - ilvG BNILDI_04735 0.37 -2.9 938,272 - ilvG BNILDI_04735 0.44 -0.6 938,417 + ilvG BNILDI_04735 0.53 -1.2 938,470 + ilvG BNILDI_04735 0.56 -3.2 938,470 + ilvG BNILDI_04735 0.56 -0.9 938,482 + ilvG BNILDI_04735 0.57 -0.9 938,482 + ilvG BNILDI_04735 0.57 -0.7 938,503 + ilvG BNILDI_04735 0.58 -1.6 938,503 + ilvG BNILDI_04735 0.58 -0.6 938,536 + ilvG BNILDI_04735 0.60 +0.1 938,594 + ilvG BNILDI_04735 0.64 -0.0 938,599 - ilvG BNILDI_04735 0.64 -2.9 938,705 + ilvG BNILDI_04735 0.71 -0.7 938,741 - ilvG BNILDI_04735 0.73 -1.8 938,813 + ilvG BNILDI_04735 0.77 -1.2 938,845 + ilvG BNILDI_04735 0.79 -0.2 938,845 + ilvG BNILDI_04735 0.79 -0.5 938,846 - ilvG BNILDI_04735 0.79 -4.8 938,871 - ilvG BNILDI_04735 0.81 -3.1 938,959 - ilvG BNILDI_04735 0.86 -3.4 939,158 + -0.2 939,159 - -4.1 939,181 + +1.0 939,181 + -0.3 939,234 + BNILDI_04740 0.19 -0.2 939,234 + BNILDI_04740 0.19 -0.0 939,234 + BNILDI_04740 0.19 -0.3 939,247 - BNILDI_04740 0.23 -1.0 939,290 - BNILDI_04740 0.40 -2.1 939,318 + BNILDI_04740 0.50 -1.2 939,319 - BNILDI_04740 0.51 -0.8 939,319 - BNILDI_04740 0.51 -0.6 939,361 - BNILDI_04740 0.67 -3.3 939,368 - BNILDI_04740 0.69 -0.7 939,375 + BNILDI_04740 0.72 -1.1 939,375 + BNILDI_04740 0.72 -0.0 939,376 - BNILDI_04740 0.72 -4.6 939,379 + BNILDI_04740 0.73 +0.1 939,379 + BNILDI_04740 0.73 -0.6 939,380 - BNILDI_04740 0.74 -2.6 939,463 + -2.6 939,489 + +1.0 939,490 - -2.9 939,561 + ilvE BNILDI_04745 0.10 -4.8 939,595 - ilvE BNILDI_04745 0.14 -4.7 939,638 + ilvE BNILDI_04745 0.18 -2.7 939,737 + ilvE BNILDI_04745 0.29 -2.6 939,738 - ilvE BNILDI_04745 0.29 -1.8 939,738 - ilvE BNILDI_04745 0.29 -5.5 939,797 - ilvE BNILDI_04745 0.35 -3.8 939,956 + ilvE BNILDI_04745 0.52 +1.6 939,961 - ilvE BNILDI_04745 0.53 -1.8 939,961 - ilvE BNILDI_04745 0.53 -1.4 939,961 - ilvE BNILDI_04745 0.53 -1.4 940,039 - ilvE BNILDI_04745 0.61 -4.0 940,040 + ilvE BNILDI_04745 0.62 -3.0 940,040 + ilvE BNILDI_04745 0.62 -2.1 940,040 + ilvE BNILDI_04745 0.62 -0.3 940,041 - ilvE BNILDI_04745 0.62 -2.2 940,041 - ilvE BNILDI_04745 0.62 -3.5 940,177 + ilvE BNILDI_04745 0.76 -3.7 940,178 - ilvE BNILDI_04745 0.76 -3.7
Or see this region's nucleotide sequence