Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uhpB and uhpA_G97D overlap by 1 nucleotides uhpA_G97D and ilvN are separated by 74 nucleotides ilvN and BNILDI_04200 are separated by 3 nucleotides BNILDI_04200 and ivbL are separated by 105 nucleotides ivbL and tisB are separated by 564 nucleotides
BNILDI_04185: uhpB - signal transduction histidine-protein kinase/phosphatase UhpB, at 830,971 to 832,473
uhpB
BNILDI_04190: uhpA_G97D - DNA-binding response regulator UhpA, at 832,473 to 833,063
_G97D
BNILDI_04195: ilvN - acetolactate synthase small subunit, at 833,138 to 833,428
ilvN
BNILDI_04200: BNILDI_04200 - Acetolactate synthase, at 833,432 to 835,120
_04200
BNILDI_04205: ivbL - ilvB operon leader peptide IvbL, at 835,226 to 835,324
ivbL
BNILDI_04215: tisB - Small toxic protein TisB, at 835,889 to 835,978
tisB
Position (kb) 833
834
835
836 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 832.451 kb on - strand at 832.451 kb on - strand at 832.506 kb on + strand at 832.507 kb on - strand at 832.515 kb on - strand at 832.680 kb on - strand, within uhpA_G97D at 832.702 kb on + strand, within uhpA_G97D at 832.703 kb on - strand, within uhpA_G97D at 832.724 kb on + strand, within uhpA_G97D at 832.724 kb on + strand, within uhpA_G97D at 832.724 kb on + strand, within uhpA_G97D at 832.867 kb on - strand, within uhpA_G97D at 832.910 kb on + strand, within uhpA_G97D at 832.911 kb on - strand, within uhpA_G97D at 832.911 kb on - strand, within uhpA_G97D at 832.977 kb on + strand, within uhpA_G97D at 833.043 kb on + strand at 833.044 kb on - strand at 833.045 kb on + strand at 833.170 kb on + strand, within ilvN at 833.170 kb on + strand, within ilvN at 833.231 kb on + strand, within ilvN at 833.275 kb on + strand, within ilvN at 833.276 kb on - strand, within ilvN at 833.276 kb on - strand, within ilvN at 833.433 kb on + strand at 833.434 kb on - strand at 833.452 kb on + strand at 833.621 kb on - strand, within BNILDI_04200 at 833.667 kb on + strand, within BNILDI_04200 at 834.018 kb on - strand, within BNILDI_04200 at 834.307 kb on + strand, within BNILDI_04200 at 834.309 kb on + strand, within BNILDI_04200 at 834.309 kb on + strand, within BNILDI_04200 at 834.310 kb on - strand, within BNILDI_04200 at 834.310 kb on - strand, within BNILDI_04200 at 834.705 kb on + strand, within BNILDI_04200 at 834.706 kb on - strand, within BNILDI_04200 at 834.739 kb on - strand, within BNILDI_04200 at 835.007 kb on - strand at 835.036 kb on + strand at 835.036 kb on + strand at 835.037 kb on - strand at 835.037 kb on - strand at 835.037 kb on - strand at 835.066 kb on + strand at 835.091 kb on - strand at 835.164 kb on - strand at 835.296 kb on - strand, within ivbL at 835.318 kb on + strand at 835.350 kb on - strand at 835.355 kb on + strand at 835.361 kb on + strand at 835.362 kb on - strand at 835.518 kb on - strand at 835.520 kb on + strand at 835.662 kb on - strand at 835.664 kb on - strand at 835.675 kb on - strand at 835.687 kb on - strand at 835.717 kb on + strand at 835.718 kb on - strand at 835.778 kb on + strand at 835.778 kb on + strand at 835.778 kb on + strand at 835.849 kb on + strand at 835.850 kb on - strand at 835.889 kb on - strand at 835.889 kb on - strand at 835.889 kb on - strand at 836.028 kb on + strand at 836.029 kb on - strand at 836.032 kb on + strand at 836.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 832,451 - -1.4 832,451 - -0.0 832,506 + -0.0 832,507 - -0.4 832,515 - +2.0 832,680 - uhpA_G97D BNILDI_04190 0.35 +0.0 832,702 + uhpA_G97D BNILDI_04190 0.39 +1.4 832,703 - uhpA_G97D BNILDI_04190 0.39 -0.3 832,724 + uhpA_G97D BNILDI_04190 0.42 -0.8 832,724 + uhpA_G97D BNILDI_04190 0.42 +0.3 832,724 + uhpA_G97D BNILDI_04190 0.42 +0.2 832,867 - uhpA_G97D BNILDI_04190 0.67 -1.0 832,910 + uhpA_G97D BNILDI_04190 0.74 +0.2 832,911 - uhpA_G97D BNILDI_04190 0.74 +0.2 832,911 - uhpA_G97D BNILDI_04190 0.74 +0.8 832,977 + uhpA_G97D BNILDI_04190 0.85 -1.3 833,043 + -0.3 833,044 - +0.2 833,045 + -0.4 833,170 + ilvN BNILDI_04195 0.11 +0.7 833,170 + ilvN BNILDI_04195 0.11 -0.3 833,231 + ilvN BNILDI_04195 0.32 +0.0 833,275 + ilvN BNILDI_04195 0.47 -1.3 833,276 - ilvN BNILDI_04195 0.47 -0.4 833,276 - ilvN BNILDI_04195 0.47 -0.9 833,433 + -1.0 833,434 - -0.5 833,452 + -3.0 833,621 - BNILDI_04200 0.11 +0.7 833,667 + BNILDI_04200 0.14 +1.0 834,018 - BNILDI_04200 0.35 -0.6 834,307 + BNILDI_04200 0.52 -0.7 834,309 + BNILDI_04200 0.52 -2.0 834,309 + BNILDI_04200 0.52 -1.8 834,310 - BNILDI_04200 0.52 -1.5 834,310 - BNILDI_04200 0.52 -2.6 834,705 + BNILDI_04200 0.75 +0.1 834,706 - BNILDI_04200 0.75 -0.5 834,739 - BNILDI_04200 0.77 -0.6 835,007 - -0.3 835,036 + -1.1 835,036 + -1.2 835,037 - -0.8 835,037 - +0.5 835,037 - -2.5 835,066 + -0.8 835,091 - -1.3 835,164 - +0.1 835,296 - ivbL BNILDI_04205 0.71 +0.3 835,318 + -0.1 835,350 - -0.4 835,355 + -0.3 835,361 + -2.1 835,362 - -2.4 835,518 - -0.2 835,520 + +0.1 835,662 - -0.6 835,664 - +0.1 835,675 - -0.1 835,687 - +0.7 835,717 + -1.4 835,718 - +0.2 835,778 + +0.1 835,778 + -0.1 835,778 + -1.1 835,849 + -0.6 835,850 - -1.1 835,889 - -0.5 835,889 - +1.1 835,889 - +0.9 836,028 + -0.4 836,029 - +1.3 836,032 + +0.4 836,033 - -0.4
Or see this region's nucleotide sequence