Strain Fitness in Escherichia coli ECRC62 around BNILDI_03970

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaJ and waaO are separated by 16 nucleotideswaaO and rfaP are separated by 15 nucleotidesrfaP and rfaG overlap by 8 nucleotides BNILDI_03965: rfaJ - lipopolysaccharide 1,2-glucosyltransferase, at 784,766 to 785,761 rfaJ BNILDI_03970: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 785,778 to 786,794 waaO BNILDI_03975: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 786,810 to 787,607 rfaP BNILDI_03980: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 787,600 to 788,724 rfaG Position (kb) 785 786 787Strain fitness (log2 ratio) -3 -2 -1 0 1at 784.809 kb on - strandat 784.840 kb on - strandat 784.963 kb on + strand, within rfaJat 785.030 kb on - strand, within rfaJat 785.296 kb on + strand, within rfaJat 786.227 kb on + strand, within waaOat 786.227 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.470 kb on + strand, within waaOat 786.500 kb on + strand, within waaOat 786.590 kb on + strand, within waaOat 786.777 kb on - strandat 786.919 kb on + strand, within rfaPat 787.116 kb on - strand, within rfaPat 787.188 kb on - strand, within rfaPat 787.274 kb on + strand, within rfaPat 787.331 kb on - strand, within rfaPat 787.621 kb on + strandat 787.655 kb on - strandat 787.665 kb on + strandat 787.666 kb on - strandat 787.674 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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784,809 - +0.6
784,840 - +1.3
784,963 + rfaJ BNILDI_03965 0.20 +1.6
785,030 - rfaJ BNILDI_03965 0.27 +0.3
785,296 + rfaJ BNILDI_03965 0.53 -2.0
786,227 + waaO BNILDI_03970 0.44 -0.1
786,227 + waaO BNILDI_03970 0.44 -3.4
786,470 + waaO BNILDI_03970 0.68 -2.6
786,470 + waaO BNILDI_03970 0.68 +0.4
786,500 + waaO BNILDI_03970 0.71 -3.6
786,590 + waaO BNILDI_03970 0.80 -2.9
786,777 - -0.5
786,919 + rfaP BNILDI_03975 0.14 -1.4
787,116 - rfaP BNILDI_03975 0.38 +1.1
787,188 - rfaP BNILDI_03975 0.47 +1.3
787,274 + rfaP BNILDI_03975 0.58 -1.8
787,331 - rfaP BNILDI_03975 0.65 -0.4
787,621 + -1.3
787,655 - -0.5
787,665 + -1.3
787,666 - +0.1
787,674 - -0.3

Or see this region's nucleotide sequence