Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rtcA and rtcB are separated by 3 nucleotides rtcB and rtcR are separated by 188 nucleotides
BNILDI_02765: rtcA - RNA 3'-terminal phosphate cyclase, at 532,558 to 533,574
rtcA
BNILDI_02770: rtcB - RNA-splicing ligase RtcB, at 533,578 to 534,804
rtcB
BNILDI_02775: rtcR - DNA-binding transcriptional regulator RtcR, at 534,993 to 536,591
rtcR
Position (kb)
533
534
535 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 532.595 kb on - strand at 532.674 kb on + strand, within rtcA at 532.727 kb on + strand, within rtcA at 532.727 kb on + strand, within rtcA at 532.872 kb on + strand, within rtcA at 532.908 kb on + strand, within rtcA at 532.908 kb on + strand, within rtcA at 532.909 kb on - strand, within rtcA at 532.945 kb on - strand, within rtcA at 532.984 kb on + strand, within rtcA at 532.984 kb on + strand, within rtcA at 532.985 kb on - strand, within rtcA at 532.987 kb on + strand, within rtcA at 532.988 kb on - strand, within rtcA at 532.988 kb on - strand, within rtcA at 533.096 kb on + strand, within rtcA at 533.110 kb on + strand, within rtcA at 533.110 kb on + strand, within rtcA at 533.110 kb on + strand, within rtcA at 533.475 kb on + strand at 533.476 kb on - strand at 533.476 kb on - strand at 533.580 kb on - strand at 533.587 kb on + strand at 533.587 kb on + strand at 533.612 kb on + strand at 533.612 kb on + strand at 533.613 kb on - strand at 533.616 kb on + strand at 533.616 kb on + strand at 533.658 kb on + strand at 533.659 kb on - strand at 533.665 kb on - strand at 533.665 kb on - strand at 533.666 kb on + strand at 533.666 kb on + strand at 533.667 kb on - strand at 533.766 kb on + strand, within rtcB at 533.957 kb on + strand, within rtcB at 533.957 kb on + strand, within rtcB at 533.957 kb on + strand, within rtcB at 533.957 kb on + strand, within rtcB at 533.958 kb on - strand, within rtcB at 533.958 kb on - strand, within rtcB at 534.040 kb on + strand, within rtcB at 534.044 kb on - strand, within rtcB at 534.111 kb on - strand, within rtcB at 534.138 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.212 kb on + strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.213 kb on - strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.335 kb on + strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.336 kb on - strand, within rtcB at 534.342 kb on + strand, within rtcB at 534.342 kb on + strand, within rtcB at 534.630 kb on + strand, within rtcB at 534.658 kb on + strand, within rtcB at 534.678 kb on + strand, within rtcB at 534.679 kb on - strand, within rtcB at 534.711 kb on + strand at 534.712 kb on - strand at 534.797 kb on + strand at 534.840 kb on - strand at 534.977 kb on + strand at 534.985 kb on + strand at 534.985 kb on + strand at 534.985 kb on + strand at 534.989 kb on + strand at 534.989 kb on + strand at 534.990 kb on - strand at 534.999 kb on - strand at 535.091 kb on - strand at 535.170 kb on - strand, within rtcR at 535.170 kb on - strand, within rtcR at 535.257 kb on + strand, within rtcR at 535.257 kb on + strand, within rtcR at 535.314 kb on + strand, within rtcR at 535.315 kb on - strand, within rtcR at 535.318 kb on + strand, within rtcR at 535.318 kb on + strand, within rtcR at 535.402 kb on + strand, within rtcR at 535.403 kb on - strand, within rtcR at 535.483 kb on - strand, within rtcR at 535.484 kb on + strand, within rtcR at 535.484 kb on + strand, within rtcR at 535.706 kb on + strand, within rtcR at 535.707 kb on - strand, within rtcR
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 532,595 - +0.7 532,674 + rtcA BNILDI_02765 0.11 +0.0 532,727 + rtcA BNILDI_02765 0.17 +0.1 532,727 + rtcA BNILDI_02765 0.17 +0.2 532,872 + rtcA BNILDI_02765 0.31 -0.2 532,908 + rtcA BNILDI_02765 0.34 +0.3 532,908 + rtcA BNILDI_02765 0.34 -0.5 532,909 - rtcA BNILDI_02765 0.35 +0.8 532,945 - rtcA BNILDI_02765 0.38 -0.3 532,984 + rtcA BNILDI_02765 0.42 +0.7 532,984 + rtcA BNILDI_02765 0.42 +0.9 532,985 - rtcA BNILDI_02765 0.42 +1.1 532,987 + rtcA BNILDI_02765 0.42 -0.1 532,988 - rtcA BNILDI_02765 0.42 +1.3 532,988 - rtcA BNILDI_02765 0.42 -0.7 533,096 + rtcA BNILDI_02765 0.53 +0.2 533,110 + rtcA BNILDI_02765 0.54 +0.0 533,110 + rtcA BNILDI_02765 0.54 +0.4 533,110 + rtcA BNILDI_02765 0.54 +1.4 533,475 + +0.0 533,476 - +0.1 533,476 - +0.0 533,580 - -0.3 533,587 + +2.9 533,587 + +0.6 533,612 + -0.7 533,612 + -2.6 533,613 - +0.3 533,616 + +1.1 533,616 + +0.9 533,658 + -0.5 533,659 - +0.2 533,665 - -0.2 533,665 - +0.1 533,666 + +0.0 533,666 + +0.3 533,667 - -0.2 533,766 + rtcB BNILDI_02770 0.15 -0.1 533,957 + rtcB BNILDI_02770 0.31 -0.9 533,957 + rtcB BNILDI_02770 0.31 -0.6 533,957 + rtcB BNILDI_02770 0.31 +0.7 533,957 + rtcB BNILDI_02770 0.31 -1.4 533,958 - rtcB BNILDI_02770 0.31 -2.3 533,958 - rtcB BNILDI_02770 0.31 +0.3 534,040 + rtcB BNILDI_02770 0.38 -1.0 534,044 - rtcB BNILDI_02770 0.38 +0.2 534,111 - rtcB BNILDI_02770 0.43 +0.4 534,138 + rtcB BNILDI_02770 0.46 +0.1 534,212 + rtcB BNILDI_02770 0.52 -0.1 534,212 + rtcB BNILDI_02770 0.52 +0.8 534,212 + rtcB BNILDI_02770 0.52 -0.6 534,212 + rtcB BNILDI_02770 0.52 -0.8 534,212 + rtcB BNILDI_02770 0.52 -0.1 534,212 + rtcB BNILDI_02770 0.52 -1.6 534,213 - rtcB BNILDI_02770 0.52 -0.2 534,213 - rtcB BNILDI_02770 0.52 -0.3 534,213 - rtcB BNILDI_02770 0.52 +0.9 534,213 - rtcB BNILDI_02770 0.52 +0.2 534,213 - rtcB BNILDI_02770 0.52 +1.0 534,335 + rtcB BNILDI_02770 0.62 +0.2 534,335 + rtcB BNILDI_02770 0.62 -0.1 534,335 + rtcB BNILDI_02770 0.62 -0.6 534,335 + rtcB BNILDI_02770 0.62 +0.1 534,335 + rtcB BNILDI_02770 0.62 +0.4 534,336 - rtcB BNILDI_02770 0.62 -0.4 534,336 - rtcB BNILDI_02770 0.62 +0.4 534,336 - rtcB BNILDI_02770 0.62 +0.0 534,342 + rtcB BNILDI_02770 0.62 +0.2 534,342 + rtcB BNILDI_02770 0.62 +0.3 534,630 + rtcB BNILDI_02770 0.86 +0.3 534,658 + rtcB BNILDI_02770 0.88 -0.2 534,678 + rtcB BNILDI_02770 0.90 -0.8 534,679 - rtcB BNILDI_02770 0.90 +0.1 534,711 + -0.9 534,712 - +0.3 534,797 + -0.9 534,840 - -2.5 534,977 + +0.4 534,985 + +0.0 534,985 + -0.5 534,985 + +2.2 534,989 + -1.3 534,989 + -0.2 534,990 - +0.1 534,999 - -1.1 535,091 - +1.4 535,170 - rtcR BNILDI_02775 0.11 +0.2 535,170 - rtcR BNILDI_02775 0.11 -1.3 535,257 + rtcR BNILDI_02775 0.17 +0.3 535,257 + rtcR BNILDI_02775 0.17 -0.3 535,314 + rtcR BNILDI_02775 0.20 -1.0 535,315 - rtcR BNILDI_02775 0.20 +0.1 535,318 + rtcR BNILDI_02775 0.20 -0.1 535,318 + rtcR BNILDI_02775 0.20 +0.3 535,402 + rtcR BNILDI_02775 0.26 -0.2 535,403 - rtcR BNILDI_02775 0.26 +1.8 535,483 - rtcR BNILDI_02775 0.31 +0.3 535,484 + rtcR BNILDI_02775 0.31 +0.2 535,484 + rtcR BNILDI_02775 0.31 -0.6 535,706 + rtcR BNILDI_02775 0.45 +0.7 535,707 - rtcR BNILDI_02775 0.45 -1.0
Or see this region's nucleotide sequence