Strain Fitness in Escherichia coli ECRC62 around BNILDI_02545

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysG and yhfL are separated by 246 nucleotidesyhfL and yhfS are separated by 106 nucleotides BNILDI_02540: cysG - siroheme synthase CysG, at 479,800 to 481,173 cysG BNILDI_02545: yhfL - Uncharacterized protein YhfL, at 481,420 to 481,587 yhfL BNILDI_02550: yhfS - Uncharacterized protein YhfS, at 481,694 to 482,779 yhfS Position (kb) 481 482Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 480.420 kb on - strand, within cysGat 480.420 kb on - strand, within cysGat 480.539 kb on - strand, within cysGat 480.564 kb on + strand, within cysGat 480.564 kb on + strand, within cysGat 480.564 kb on + strand, within cysGat 480.564 kb on + strand, within cysGat 480.565 kb on - strand, within cysGat 480.565 kb on - strand, within cysGat 480.620 kb on - strand, within cysGat 480.630 kb on - strand, within cysGat 480.844 kb on + strand, within cysGat 480.864 kb on - strand, within cysGat 480.864 kb on - strand, within cysGat 480.868 kb on - strand, within cysGat 480.940 kb on + strand, within cysGat 480.940 kb on + strand, within cysGat 480.940 kb on + strand, within cysGat 480.941 kb on - strand, within cysGat 480.941 kb on - strand, within cysGat 480.941 kb on - strand, within cysGat 481.063 kb on - strandat 481.112 kb on + strandat 481.116 kb on + strandat 481.117 kb on - strandat 481.117 kb on - strandat 481.120 kb on - strandat 481.241 kb on - strandat 481.251 kb on + strandat 481.449 kb on - strand, within yhfLat 481.479 kb on + strand, within yhfLat 481.517 kb on - strand, within yhfLat 481.654 kb on - strandat 481.696 kb on - strandat 481.957 kb on + strand, within yhfSat 481.957 kb on + strand, within yhfSat 482.075 kb on + strand, within yhfSat 482.075 kb on + strand, within yhfSat 482.118 kb on + strand, within yhfSat 482.119 kb on - strand, within yhfSat 482.135 kb on - strand, within yhfSat 482.190 kb on - strand, within yhfSat 482.262 kb on + strand, within yhfSat 482.262 kb on + strand, within yhfSat 482.263 kb on - strand, within yhfSat 482.328 kb on + strand, within yhfSat 482.353 kb on + strand, within yhfSat 482.434 kb on + strand, within yhfSat 482.458 kb on + strand, within yhfSat 482.462 kb on - strand, within yhfS

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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480,420 - cysG BNILDI_02540 0.45 -0.6
480,420 - cysG BNILDI_02540 0.45 -2.7
480,539 - cysG BNILDI_02540 0.54 -1.2
480,564 + cysG BNILDI_02540 0.56 -1.5
480,564 + cysG BNILDI_02540 0.56 -0.8
480,564 + cysG BNILDI_02540 0.56 -2.2
480,564 + cysG BNILDI_02540 0.56 -3.0
480,565 - cysG BNILDI_02540 0.56 -2.5
480,565 - cysG BNILDI_02540 0.56 -2.1
480,620 - cysG BNILDI_02540 0.60 -2.5
480,630 - cysG BNILDI_02540 0.60 -0.3
480,844 + cysG BNILDI_02540 0.76 -0.3
480,864 - cysG BNILDI_02540 0.77 -1.8
480,864 - cysG BNILDI_02540 0.77 -2.1
480,868 - cysG BNILDI_02540 0.78 -2.0
480,940 + cysG BNILDI_02540 0.83 -1.9
480,940 + cysG BNILDI_02540 0.83 -1.5
480,940 + cysG BNILDI_02540 0.83 -1.4
480,941 - cysG BNILDI_02540 0.83 -1.7
480,941 - cysG BNILDI_02540 0.83 -2.6
480,941 - cysG BNILDI_02540 0.83 -0.8
481,063 - -2.0
481,112 + -1.4
481,116 + -1.9
481,117 - -1.4
481,117 - -1.7
481,120 - -0.3
481,241 - -0.8
481,251 + +0.5
481,449 - yhfL BNILDI_02545 0.17 +0.2
481,479 + yhfL BNILDI_02545 0.35 -0.0
481,517 - yhfL BNILDI_02545 0.58 +2.4
481,654 - +0.2
481,696 - +0.3
481,957 + yhfS BNILDI_02550 0.24 -0.4
481,957 + yhfS BNILDI_02550 0.24 +0.8
482,075 + yhfS BNILDI_02550 0.35 +0.3
482,075 + yhfS BNILDI_02550 0.35 -0.1
482,118 + yhfS BNILDI_02550 0.39 -0.0
482,119 - yhfS BNILDI_02550 0.39 +0.3
482,135 - yhfS BNILDI_02550 0.41 +0.6
482,190 - yhfS BNILDI_02550 0.46 -0.1
482,262 + yhfS BNILDI_02550 0.52 +0.1
482,262 + yhfS BNILDI_02550 0.52 -2.6
482,263 - yhfS BNILDI_02550 0.52 -0.9
482,328 + yhfS BNILDI_02550 0.58 +0.2
482,353 + yhfS BNILDI_02550 0.61 +0.2
482,434 + yhfS BNILDI_02550 0.68 +0.7
482,458 + yhfS BNILDI_02550 0.70 +0.0
482,462 - yhfS BNILDI_02550 0.71 +0.0

Or see this region's nucleotide sequence