Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nirD and nirC are separated by 126 nucleotides nirC and cysG are separated by 18 nucleotides cysG and yhfL are separated by 246 nucleotides yhfL and yhfS are separated by 106 nucleotides
BNILDI_02530: nirD - nitrite reductase small subunit NirD, at 478,522 to 478,848
nirD
BNILDI_02535: nirC - nitrite transporter NirC, at 478,975 to 479,781
nirC
BNILDI_02540: cysG - siroheme synthase CysG, at 479,800 to 481,173
cysG
BNILDI_02545: yhfL - Uncharacterized protein YhfL, at 481,420 to 481,587
yhfL
BNILDI_02550: yhfS - Uncharacterized protein YhfS, at 481,694 to 482,779
yhfS
Position (kb) 479
480
481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 478.804 kb on + strand, within nirD at 478.854 kb on + strand at 478.894 kb on + strand at 478.907 kb on + strand at 478.989 kb on + strand at 479.083 kb on + strand, within nirC at 479.141 kb on + strand, within nirC at 479.141 kb on + strand, within nirC at 479.341 kb on + strand, within nirC at 479.341 kb on + strand, within nirC at 479.342 kb on - strand, within nirC at 479.342 kb on - strand, within nirC at 479.343 kb on + strand, within nirC at 479.343 kb on + strand, within nirC at 479.344 kb on - strand, within nirC at 479.376 kb on + strand, within nirC at 479.377 kb on - strand, within nirC at 479.377 kb on - strand, within nirC at 479.521 kb on - strand, within nirC at 479.544 kb on + strand, within nirC at 479.623 kb on + strand, within nirC at 479.624 kb on - strand, within nirC at 479.674 kb on - strand, within nirC at 479.707 kb on + strand at 479.718 kb on + strand at 479.718 kb on + strand at 479.755 kb on - strand at 479.783 kb on + strand at 479.783 kb on + strand at 479.783 kb on + strand at 479.784 kb on - strand at 479.784 kb on - strand at 479.786 kb on + strand at 479.787 kb on - strand at 479.935 kb on - strand at 480.099 kb on + strand, within cysG at 480.133 kb on - strand, within cysG at 480.135 kb on + strand, within cysG at 480.135 kb on + strand, within cysG at 480.135 kb on + strand, within cysG at 480.136 kb on - strand, within cysG at 480.282 kb on - strand, within cysG at 480.282 kb on - strand, within cysG at 480.400 kb on - strand, within cysG at 480.419 kb on + strand, within cysG at 480.420 kb on - strand, within cysG at 480.420 kb on - strand, within cysG at 480.539 kb on - strand, within cysG at 480.564 kb on + strand, within cysG at 480.564 kb on + strand, within cysG at 480.564 kb on + strand, within cysG at 480.564 kb on + strand, within cysG at 480.565 kb on - strand, within cysG at 480.565 kb on - strand, within cysG at 480.620 kb on - strand, within cysG at 480.630 kb on - strand, within cysG at 480.844 kb on + strand, within cysG at 480.864 kb on - strand, within cysG at 480.864 kb on - strand, within cysG at 480.868 kb on - strand, within cysG at 480.940 kb on + strand, within cysG at 480.940 kb on + strand, within cysG at 480.940 kb on + strand, within cysG at 480.941 kb on - strand, within cysG at 480.941 kb on - strand, within cysG at 480.941 kb on - strand, within cysG at 481.063 kb on - strand at 481.112 kb on + strand at 481.116 kb on + strand at 481.117 kb on - strand at 481.117 kb on - strand at 481.120 kb on - strand at 481.241 kb on - strand at 481.251 kb on + strand at 481.449 kb on - strand, within yhfL at 481.479 kb on + strand, within yhfL at 481.517 kb on - strand, within yhfL at 481.654 kb on - strand at 481.696 kb on - strand at 481.957 kb on + strand, within yhfS at 481.957 kb on + strand, within yhfS at 482.075 kb on + strand, within yhfS at 482.075 kb on + strand, within yhfS at 482.118 kb on + strand, within yhfS at 482.119 kb on - strand, within yhfS at 482.135 kb on - strand, within yhfS
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 478,804 + nirD BNILDI_02530 0.86 +2.1 478,854 + +1.1 478,894 + +0.2 478,907 + -0.3 478,989 + -0.4 479,083 + nirC BNILDI_02535 0.13 -0.4 479,141 + nirC BNILDI_02535 0.21 +0.6 479,141 + nirC BNILDI_02535 0.21 -1.2 479,341 + nirC BNILDI_02535 0.45 -0.3 479,341 + nirC BNILDI_02535 0.45 +0.4 479,342 - nirC BNILDI_02535 0.45 +0.5 479,342 - nirC BNILDI_02535 0.45 +0.3 479,343 + nirC BNILDI_02535 0.46 +0.3 479,343 + nirC BNILDI_02535 0.46 +0.5 479,344 - nirC BNILDI_02535 0.46 -1.5 479,376 + nirC BNILDI_02535 0.50 -1.0 479,377 - nirC BNILDI_02535 0.50 -0.9 479,377 - nirC BNILDI_02535 0.50 +1.2 479,521 - nirC BNILDI_02535 0.68 -0.1 479,544 + nirC BNILDI_02535 0.71 -1.2 479,623 + nirC BNILDI_02535 0.80 -0.2 479,624 - nirC BNILDI_02535 0.80 -1.4 479,674 - nirC BNILDI_02535 0.87 +1.0 479,707 + -0.5 479,718 + -0.4 479,718 + -0.2 479,755 - +1.7 479,783 + +0.0 479,783 + -0.1 479,783 + +0.0 479,784 - +1.3 479,784 - +0.0 479,786 + -1.2 479,787 - -0.1 479,935 - -1.6 480,099 + cysG BNILDI_02540 0.22 -1.0 480,133 - cysG BNILDI_02540 0.24 -1.0 480,135 + cysG BNILDI_02540 0.24 -2.1 480,135 + cysG BNILDI_02540 0.24 -0.1 480,135 + cysG BNILDI_02540 0.24 -1.1 480,136 - cysG BNILDI_02540 0.24 -3.9 480,282 - cysG BNILDI_02540 0.35 -1.9 480,282 - cysG BNILDI_02540 0.35 -1.5 480,400 - cysG BNILDI_02540 0.44 -3.0 480,419 + cysG BNILDI_02540 0.45 -1.9 480,420 - cysG BNILDI_02540 0.45 -0.6 480,420 - cysG BNILDI_02540 0.45 -2.7 480,539 - cysG BNILDI_02540 0.54 -1.2 480,564 + cysG BNILDI_02540 0.56 -1.5 480,564 + cysG BNILDI_02540 0.56 -0.8 480,564 + cysG BNILDI_02540 0.56 -2.2 480,564 + cysG BNILDI_02540 0.56 -3.0 480,565 - cysG BNILDI_02540 0.56 -2.5 480,565 - cysG BNILDI_02540 0.56 -2.1 480,620 - cysG BNILDI_02540 0.60 -2.5 480,630 - cysG BNILDI_02540 0.60 -0.3 480,844 + cysG BNILDI_02540 0.76 -0.3 480,864 - cysG BNILDI_02540 0.77 -1.8 480,864 - cysG BNILDI_02540 0.77 -2.1 480,868 - cysG BNILDI_02540 0.78 -2.0 480,940 + cysG BNILDI_02540 0.83 -1.9 480,940 + cysG BNILDI_02540 0.83 -1.5 480,940 + cysG BNILDI_02540 0.83 -1.4 480,941 - cysG BNILDI_02540 0.83 -1.7 480,941 - cysG BNILDI_02540 0.83 -2.6 480,941 - cysG BNILDI_02540 0.83 -0.8 481,063 - -2.0 481,112 + -1.4 481,116 + -1.9 481,117 - -1.4 481,117 - -1.7 481,120 - -0.3 481,241 - -0.8 481,251 + +0.5 481,449 - yhfL BNILDI_02545 0.17 +0.2 481,479 + yhfL BNILDI_02545 0.35 -0.0 481,517 - yhfL BNILDI_02545 0.58 +2.4 481,654 - +0.2 481,696 - +0.3 481,957 + yhfS BNILDI_02550 0.24 -0.4 481,957 + yhfS BNILDI_02550 0.24 +0.8 482,075 + yhfS BNILDI_02550 0.35 +0.3 482,075 + yhfS BNILDI_02550 0.35 -0.1 482,118 + yhfS BNILDI_02550 0.39 -0.0 482,119 - yhfS BNILDI_02550 0.39 +0.3 482,135 - yhfS BNILDI_02550 0.41 +0.6
Or see this region's nucleotide sequence