Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slyX and slyD are separated by 48 nucleotides slyD and yheV are separated by 94 nucleotides yheV and kefB are separated by 9 nucleotides kefB and kefG overlap by 1 nucleotides kefG and yheS are separated by 130 nucleotides
BNILDI_02430: slyX - Protein SlyX, at 459,611 to 459,829
slyX
BNILDI_02435: slyD - peptidylprolyl isomerase, at 459,878 to 460,468
slyD
BNILDI_02440: yheV - Uncharacterized protein YheV, at 460,563 to 460,763
yheV
BNILDI_02445: kefB - glutathione-regulated potassium-efflux system protein KefB, at 460,773 to 462,578
kefB
BNILDI_02450: kefG - glutathione-regulated potassium-efflux system ancillary protein KefG, at 462,578 to 463,129
kefG
BNILDI_02455: yheS - ABC transporter ATP-binding protein, at 463,260 to 465,173
yheS
Position (kb) 460
461
462
463 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 459.908 kb on - strand at 460.189 kb on + strand, within slyD at 460.190 kb on - strand, within slyD at 460.190 kb on - strand, within slyD at 460.193 kb on + strand, within slyD at 460.193 kb on + strand, within slyD at 460.193 kb on + strand, within slyD at 460.194 kb on - strand, within slyD at 460.221 kb on - strand, within slyD at 460.221 kb on - strand, within slyD at 460.221 kb on - strand, within slyD at 460.228 kb on + strand, within slyD at 460.229 kb on - strand, within slyD at 460.369 kb on - strand, within slyD at 460.420 kb on + strand at 460.420 kb on + strand at 460.421 kb on - strand at 460.421 kb on - strand at 460.424 kb on + strand at 460.424 kb on + strand at 460.424 kb on + strand at 460.424 kb on + strand at 460.424 kb on + strand at 460.431 kb on + strand at 460.471 kb on + strand at 460.471 kb on + strand at 460.677 kb on - strand, within yheV at 460.679 kb on + strand, within yheV at 460.768 kb on - strand at 460.902 kb on + strand at 461.099 kb on + strand, within kefB at 461.168 kb on + strand, within kefB at 461.169 kb on - strand, within kefB at 461.169 kb on - strand, within kefB at 461.237 kb on - strand, within kefB at 461.254 kb on + strand, within kefB at 461.254 kb on + strand, within kefB at 461.301 kb on - strand, within kefB at 461.448 kb on - strand, within kefB at 461.632 kb on + strand, within kefB at 461.650 kb on + strand, within kefB at 461.716 kb on + strand, within kefB at 461.716 kb on + strand, within kefB at 461.716 kb on + strand, within kefB at 461.716 kb on + strand, within kefB at 461.717 kb on - strand, within kefB at 461.717 kb on - strand, within kefB at 461.717 kb on - strand, within kefB at 461.753 kb on + strand, within kefB at 461.846 kb on + strand, within kefB at 461.846 kb on + strand, within kefB at 461.853 kb on - strand, within kefB at 461.886 kb on - strand, within kefB at 461.958 kb on - strand, within kefB at 461.984 kb on - strand, within kefB at 461.984 kb on - strand, within kefB at 462.265 kb on - strand, within kefB at 462.288 kb on + strand, within kefB at 462.384 kb on + strand, within kefB at 462.432 kb on - strand at 462.677 kb on - strand, within kefG at 462.677 kb on - strand, within kefG at 462.677 kb on - strand, within kefG at 462.802 kb on + strand, within kefG at 462.811 kb on + strand, within kefG at 462.869 kb on + strand, within kefG at 462.869 kb on + strand, within kefG at 462.869 kb on + strand, within kefG at 462.870 kb on - strand, within kefG at 462.910 kb on + strand, within kefG at 462.911 kb on - strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.912 kb on + strand, within kefG at 462.913 kb on - strand, within kefG at 462.913 kb on - strand, within kefG at 462.913 kb on - strand, within kefG at 462.913 kb on - strand, within kefG at 462.916 kb on + strand, within kefG at 462.916 kb on + strand, within kefG at 462.917 kb on - strand, within kefG at 462.919 kb on - strand, within kefG at 462.979 kb on + strand, within kefG at 463.052 kb on - strand, within kefG at 463.098 kb on + strand at 463.098 kb on + strand at 463.098 kb on + strand at 463.099 kb on - strand at 463.099 kb on - strand at 463.158 kb on - strand at 463.160 kb on - strand at 463.160 kb on - strand at 463.259 kb on + strand at 463.259 kb on + strand at 463.259 kb on + strand at 463.260 kb on - strand at 463.260 kb on - strand at 463.377 kb on - strand at 463.382 kb on + strand at 463.506 kb on + strand, within yheS at 463.506 kb on + strand, within yheS at 463.506 kb on + strand, within yheS at 463.507 kb on - strand, within yheS at 463.541 kb on - strand, within yheS at 463.553 kb on - strand, within yheS at 463.572 kb on - strand, within yheS
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 459,908 - +0.8 460,189 + slyD BNILDI_02435 0.53 +0.2 460,190 - slyD BNILDI_02435 0.53 +1.0 460,190 - slyD BNILDI_02435 0.53 -0.2 460,193 + slyD BNILDI_02435 0.53 -0.3 460,193 + slyD BNILDI_02435 0.53 -0.5 460,193 + slyD BNILDI_02435 0.53 -0.3 460,194 - slyD BNILDI_02435 0.53 -0.9 460,221 - slyD BNILDI_02435 0.58 -1.0 460,221 - slyD BNILDI_02435 0.58 +1.0 460,221 - slyD BNILDI_02435 0.58 +0.0 460,228 + slyD BNILDI_02435 0.59 +0.3 460,229 - slyD BNILDI_02435 0.59 +1.4 460,369 - slyD BNILDI_02435 0.83 +1.5 460,420 + +0.8 460,420 + -0.1 460,421 - +0.3 460,421 - -0.1 460,424 + +0.1 460,424 + +0.1 460,424 + -1.7 460,424 + -0.2 460,424 + +0.6 460,431 + -0.2 460,471 + +0.3 460,471 + -1.6 460,677 - yheV BNILDI_02440 0.57 -0.4 460,679 + yheV BNILDI_02440 0.58 +0.1 460,768 - -0.0 460,902 + +0.8 461,099 + kefB BNILDI_02445 0.18 +0.1 461,168 + kefB BNILDI_02445 0.22 +0.6 461,169 - kefB BNILDI_02445 0.22 +0.5 461,169 - kefB BNILDI_02445 0.22 +1.2 461,237 - kefB BNILDI_02445 0.26 -0.8 461,254 + kefB BNILDI_02445 0.27 +0.3 461,254 + kefB BNILDI_02445 0.27 -0.3 461,301 - kefB BNILDI_02445 0.29 +0.1 461,448 - kefB BNILDI_02445 0.37 -0.6 461,632 + kefB BNILDI_02445 0.48 -0.5 461,650 + kefB BNILDI_02445 0.49 +0.1 461,716 + kefB BNILDI_02445 0.52 +0.1 461,716 + kefB BNILDI_02445 0.52 -0.1 461,716 + kefB BNILDI_02445 0.52 +0.2 461,716 + kefB BNILDI_02445 0.52 -0.0 461,717 - kefB BNILDI_02445 0.52 -0.3 461,717 - kefB BNILDI_02445 0.52 -0.1 461,717 - kefB BNILDI_02445 0.52 +1.2 461,753 + kefB BNILDI_02445 0.54 -1.1 461,846 + kefB BNILDI_02445 0.59 +0.6 461,846 + kefB BNILDI_02445 0.59 +0.0 461,853 - kefB BNILDI_02445 0.60 -1.6 461,886 - kefB BNILDI_02445 0.62 -2.9 461,958 - kefB BNILDI_02445 0.66 +0.2 461,984 - kefB BNILDI_02445 0.67 +0.1 461,984 - kefB BNILDI_02445 0.67 -0.2 462,265 - kefB BNILDI_02445 0.83 -0.4 462,288 + kefB BNILDI_02445 0.84 -0.0 462,384 + kefB BNILDI_02445 0.89 +0.2 462,432 - +0.2 462,677 - kefG BNILDI_02450 0.18 -0.1 462,677 - kefG BNILDI_02450 0.18 +0.2 462,677 - kefG BNILDI_02450 0.18 -3.1 462,802 + kefG BNILDI_02450 0.41 +0.5 462,811 + kefG BNILDI_02450 0.42 +0.0 462,869 + kefG BNILDI_02450 0.53 +0.5 462,869 + kefG BNILDI_02450 0.53 -0.7 462,869 + kefG BNILDI_02450 0.53 -1.2 462,870 - kefG BNILDI_02450 0.53 -0.7 462,910 + kefG BNILDI_02450 0.60 +0.3 462,911 - kefG BNILDI_02450 0.60 -0.4 462,912 + kefG BNILDI_02450 0.61 +0.7 462,912 + kefG BNILDI_02450 0.61 +0.3 462,912 + kefG BNILDI_02450 0.61 -0.1 462,912 + kefG BNILDI_02450 0.61 +0.6 462,912 + kefG BNILDI_02450 0.61 -0.5 462,912 + kefG BNILDI_02450 0.61 +0.2 462,912 + kefG BNILDI_02450 0.61 +1.1 462,913 - kefG BNILDI_02450 0.61 +0.6 462,913 - kefG BNILDI_02450 0.61 +0.6 462,913 - kefG BNILDI_02450 0.61 +0.0 462,913 - kefG BNILDI_02450 0.61 -1.4 462,916 + kefG BNILDI_02450 0.61 -0.4 462,916 + kefG BNILDI_02450 0.61 -0.2 462,917 - kefG BNILDI_02450 0.61 +0.2 462,919 - kefG BNILDI_02450 0.62 -3.3 462,979 + kefG BNILDI_02450 0.73 +1.4 463,052 - kefG BNILDI_02450 0.86 +0.9 463,098 + -0.1 463,098 + +0.1 463,098 + +0.6 463,099 - -0.7 463,099 - +0.4 463,158 - -0.1 463,160 - -0.1 463,160 - -0.4 463,259 + -0.1 463,259 + -0.2 463,259 + -1.5 463,260 - +0.5 463,260 - -0.1 463,377 - +1.0 463,382 + +0.4 463,506 + yheS BNILDI_02455 0.13 -0.1 463,506 + yheS BNILDI_02455 0.13 -0.8 463,506 + yheS BNILDI_02455 0.13 -0.0 463,507 - yheS BNILDI_02455 0.13 -1.9 463,541 - yheS BNILDI_02455 0.15 +0.1 463,553 - yheS BNILDI_02455 0.15 -0.2 463,572 - yheS BNILDI_02455 0.16 +0.4
Or see this region's nucleotide sequence