Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmB and trkA are separated by 21 nucleotides trkA and mscL are separated by 129 nucleotides mscL and arfA overlap by 4 nucleotides arfA and zntR are separated by 55 nucleotides
BNILDI_02200: rsmB - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 432,284 to 433,573
rsmB
BNILDI_02205: trkA - Trk system potassium transporter TrkA, at 433,595 to 434,971
trkA
BNILDI_02210: mscL - large-conductance mechanosensitive channel protein MscL, at 435,101 to 435,511
mscL
BNILDI_02215: arfA - alternative ribosome-rescue factor ArfA, at 435,508 to 435,726
arfA
BNILDI_02220: zntR - Zn(2+)-responsive transcriptional regulator, at 435,782 to 436,207
zntR
Position (kb) 433
434
435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 432.678 kb on + strand, within rsmB at 432.679 kb on - strand, within rsmB at 433.073 kb on - strand, within rsmB at 433.153 kb on - strand, within rsmB at 433.294 kb on + strand, within rsmB at 433.374 kb on + strand, within rsmB at 433.375 kb on - strand, within rsmB at 433.442 kb on - strand, within rsmB at 433.459 kb on + strand at 433.550 kb on + strand at 434.075 kb on + strand, within trkA at 434.126 kb on - strand, within trkA at 434.290 kb on + strand, within trkA at 434.969 kb on + strand at 434.974 kb on - strand at 434.976 kb on + strand at 435.066 kb on - strand at 435.066 kb on - strand at 435.072 kb on - strand at 435.199 kb on - strand, within mscL at 435.388 kb on + strand, within mscL at 435.388 kb on + strand, within mscL at 435.448 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.459 kb on + strand, within mscL at 435.462 kb on + strand, within mscL at 435.509 kb on + strand at 435.509 kb on + strand at 435.510 kb on - strand at 435.612 kb on - strand, within arfA at 435.612 kb on - strand, within arfA at 435.671 kb on - strand, within arfA at 435.680 kb on - strand, within arfA at 435.705 kb on + strand at 435.705 kb on + strand at 435.706 kb on - strand at 435.709 kb on - strand at 435.726 kb on + strand at 435.840 kb on + strand, within zntR at 435.841 kb on - strand, within zntR at 435.901 kb on + strand, within zntR at 435.901 kb on + strand, within zntR
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 432,678 + rsmB BNILDI_02200 0.31 -1.1 432,679 - rsmB BNILDI_02200 0.31 -2.0 433,073 - rsmB BNILDI_02200 0.61 -2.3 433,153 - rsmB BNILDI_02200 0.67 -0.6 433,294 + rsmB BNILDI_02200 0.78 -1.2 433,374 + rsmB BNILDI_02200 0.84 -0.9 433,375 - rsmB BNILDI_02200 0.85 -0.6 433,442 - rsmB BNILDI_02200 0.90 -0.2 433,459 + -0.3 433,550 + -0.2 434,075 + trkA BNILDI_02205 0.35 -3.4 434,126 - trkA BNILDI_02205 0.39 -0.1 434,290 + trkA BNILDI_02205 0.50 -0.7 434,969 + +1.0 434,974 - +1.8 434,976 + +1.1 435,066 - -0.7 435,066 - +0.1 435,072 - +0.1 435,199 - mscL BNILDI_02210 0.24 +0.4 435,388 + mscL BNILDI_02210 0.70 -0.9 435,388 + mscL BNILDI_02210 0.70 -1.4 435,448 + mscL BNILDI_02210 0.84 -1.0 435,459 + mscL BNILDI_02210 0.87 +0.4 435,459 + mscL BNILDI_02210 0.87 +0.5 435,459 + mscL BNILDI_02210 0.87 +1.4 435,462 + mscL BNILDI_02210 0.88 +1.4 435,509 + -1.8 435,509 + -0.2 435,510 - +0.3 435,612 - arfA BNILDI_02215 0.47 -0.4 435,612 - arfA BNILDI_02215 0.47 -0.2 435,671 - arfA BNILDI_02215 0.74 -0.0 435,680 - arfA BNILDI_02215 0.79 +0.3 435,705 + +1.1 435,705 + -0.5 435,706 - +0.3 435,709 - -0.1 435,726 + -2.4 435,840 + zntR BNILDI_02220 0.14 +1.9 435,841 - zntR BNILDI_02220 0.14 +0.3 435,901 + zntR BNILDI_02220 0.28 -1.3 435,901 + zntR BNILDI_02220 0.28 +0.5
Or see this region's nucleotide sequence