Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmE and yhbY are separated by 125 nucleotides yhbY and greA are separated by 155 nucleotides greA and dacB are separated by 247 nucleotides
BNILDI_01680: rlmE - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 331,556 to 332,185
rlmE
BNILDI_01685: yhbY - ribosome assembly RNA-binding protein YhbY, at 332,311 to 332,604
yhbY
BNILDI_01690: greA - transcription elongation factor GreA, at 332,760 to 333,236
greA
BNILDI_01695: dacB - serine-type D-Ala-D-Ala carboxypeptidase, at 333,484 to 334,917
dacB
Position (kb) 332
333
334 Strain fitness (log2 ratio) -2
-1
0
1
2 at 332.177 kb on - strand at 332.253 kb on - strand at 332.253 kb on - strand at 332.253 kb on - strand at 332.253 kb on - strand at 332.253 kb on - strand at 332.255 kb on - strand at 332.325 kb on + strand at 332.326 kb on - strand at 332.388 kb on + strand, within yhbY at 332.432 kb on + strand, within yhbY at 332.485 kb on - strand, within yhbY at 332.506 kb on - strand, within yhbY at 332.506 kb on - strand, within yhbY at 332.529 kb on + strand, within yhbY at 332.529 kb on + strand, within yhbY at 332.534 kb on + strand, within yhbY at 332.535 kb on - strand, within yhbY at 332.535 kb on - strand, within yhbY at 332.574 kb on + strand, within yhbY at 332.574 kb on + strand, within yhbY at 332.636 kb on + strand at 332.692 kb on + strand at 332.777 kb on + strand at 332.778 kb on - strand at 332.778 kb on - strand at 332.781 kb on + strand at 332.782 kb on - strand at 332.794 kb on - strand at 332.892 kb on - strand, within greA at 332.924 kb on - strand, within greA at 333.094 kb on + strand, within greA at 333.241 kb on + strand at 333.241 kb on + strand at 333.242 kb on - strand at 333.243 kb on + strand at 333.244 kb on - strand at 333.244 kb on - strand at 333.424 kb on - strand at 333.431 kb on - strand at 333.445 kb on + strand at 333.448 kb on + strand at 333.522 kb on + strand at 333.523 kb on - strand at 333.523 kb on - strand at 333.524 kb on + strand at 333.559 kb on + strand at 333.684 kb on + strand, within dacB at 333.684 kb on + strand, within dacB at 333.785 kb on + strand, within dacB at 333.786 kb on - strand, within dacB at 333.786 kb on - strand, within dacB at 333.893 kb on + strand, within dacB at 333.893 kb on + strand, within dacB at 333.894 kb on - strand, within dacB at 333.894 kb on - strand, within dacB at 333.986 kb on + strand, within dacB at 333.986 kb on + strand, within dacB at 333.986 kb on + strand, within dacB at 333.987 kb on - strand, within dacB at 333.987 kb on - strand, within dacB at 334.018 kb on + strand, within dacB at 334.018 kb on + strand, within dacB at 334.019 kb on - strand, within dacB at 334.041 kb on + strand, within dacB at 334.051 kb on - strand, within dacB at 334.067 kb on - strand, within dacB at 334.067 kb on - strand, within dacB at 334.094 kb on + strand, within dacB
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 332,177 - -1.4 332,253 - +0.2 332,253 - +0.3 332,253 - -0.2 332,253 - +0.3 332,253 - -0.9 332,255 - -0.5 332,325 + +0.2 332,326 - +2.1 332,388 + yhbY BNILDI_01685 0.26 +2.1 332,432 + yhbY BNILDI_01685 0.41 +0.2 332,485 - yhbY BNILDI_01685 0.59 -0.1 332,506 - yhbY BNILDI_01685 0.66 -0.3 332,506 - yhbY BNILDI_01685 0.66 -0.1 332,529 + yhbY BNILDI_01685 0.74 -0.5 332,529 + yhbY BNILDI_01685 0.74 +1.5 332,534 + yhbY BNILDI_01685 0.76 +0.2 332,535 - yhbY BNILDI_01685 0.76 -0.9 332,535 - yhbY BNILDI_01685 0.76 +0.8 332,574 + yhbY BNILDI_01685 0.89 +0.3 332,574 + yhbY BNILDI_01685 0.89 +0.8 332,636 + -1.1 332,692 + +0.1 332,777 + -0.0 332,778 - -0.7 332,778 - -1.2 332,781 + +0.8 332,782 - +0.1 332,794 - +0.2 332,892 - greA BNILDI_01690 0.28 -0.1 332,924 - greA BNILDI_01690 0.34 -1.2 333,094 + greA BNILDI_01690 0.70 -1.9 333,241 + -0.2 333,241 + +0.9 333,242 - -1.8 333,243 + +0.5 333,244 - +0.4 333,244 - -0.5 333,424 - +0.0 333,431 - +1.2 333,445 + +1.2 333,448 + +0.9 333,522 + +0.4 333,523 - +0.5 333,523 - -0.2 333,524 + +0.9 333,559 + +0.8 333,684 + dacB BNILDI_01695 0.14 -0.1 333,684 + dacB BNILDI_01695 0.14 +1.0 333,785 + dacB BNILDI_01695 0.21 +1.3 333,786 - dacB BNILDI_01695 0.21 -0.1 333,786 - dacB BNILDI_01695 0.21 +1.0 333,893 + dacB BNILDI_01695 0.29 -0.3 333,893 + dacB BNILDI_01695 0.29 -0.8 333,894 - dacB BNILDI_01695 0.29 -0.1 333,894 - dacB BNILDI_01695 0.29 +0.4 333,986 + dacB BNILDI_01695 0.35 +0.1 333,986 + dacB BNILDI_01695 0.35 +0.5 333,986 + dacB BNILDI_01695 0.35 -0.1 333,987 - dacB BNILDI_01695 0.35 -1.0 333,987 - dacB BNILDI_01695 0.35 -0.4 334,018 + dacB BNILDI_01695 0.37 +1.4 334,018 + dacB BNILDI_01695 0.37 +0.3 334,019 - dacB BNILDI_01695 0.37 -0.2 334,041 + dacB BNILDI_01695 0.39 -0.5 334,051 - dacB BNILDI_01695 0.40 +0.9 334,067 - dacB BNILDI_01695 0.41 +0.2 334,067 - dacB BNILDI_01695 0.41 +0.3 334,094 + dacB BNILDI_01695 0.43 +1.1
Or see this region's nucleotide sequence