Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yraQ and yraR are separated by 72 nucleotides yraR and yhbO are separated by 127 nucleotides yhbO and BNILDI_01535 are separated by 153 nucleotides BNILDI_01535 and yhbQ are separated by 50 nucleotides yhbQ and yhbS overlap by 14 nucleotides
BNILDI_01520: yraQ - UPF0718 protein YraQ, at 300,215 to 301,255
yraQ
BNILDI_01525: yraR - Putative NAD(P)-binding protein YraR, at 301,328 to 301,963
yraR
BNILDI_01530: yhbO - protein/nucleic acid deglycase, at 302,091 to 302,609
yhbO
BNILDI_01535: BNILDI_01535 - UPF0306 protein, at 302,763 to 303,032
_01535
BNILDI_01540: yhbQ - DNA damage response exodeoxyribonuclease YhbQ, at 303,083 to 303,385
yhbQ
BNILDI_01545: yhbS - Uncharacterized N-acetyltransferase YhbS, at 303,372 to 303,875
yhbS
Position (kb) 302
303 Strain fitness (log2 ratio) -2
-1
0
1 at 301.097 kb on - strand, within yraQ at 301.129 kb on + strand, within yraQ at 301.164 kb on + strand at 301.255 kb on + strand at 301.255 kb on + strand at 301.272 kb on - strand at 301.276 kb on + strand at 301.277 kb on - strand at 301.298 kb on - strand at 301.458 kb on - strand, within yraR at 301.458 kb on - strand, within yraR at 301.505 kb on - strand, within yraR at 301.648 kb on + strand, within yraR at 301.648 kb on + strand, within yraR at 301.649 kb on - strand, within yraR at 301.829 kb on - strand, within yraR at 301.953 kb on - strand at 302.020 kb on - strand at 302.020 kb on - strand at 302.034 kb on + strand at 302.035 kb on - strand at 302.097 kb on - strand at 302.144 kb on + strand, within yhbO at 302.144 kb on + strand, within yhbO at 302.325 kb on + strand, within yhbO at 302.357 kb on + strand, within yhbO at 302.732 kb on - strand at 302.786 kb on + strand at 302.806 kb on - strand, within BNILDI_01535 at 302.902 kb on + strand, within BNILDI_01535 at 303.047 kb on - strand at 303.047 kb on - strand at 303.048 kb on + strand at 303.048 kb on + strand at 303.048 kb on + strand at 303.049 kb on - strand at 303.065 kb on + strand at 303.102 kb on - strand at 303.131 kb on + strand, within yhbQ at 303.132 kb on - strand, within yhbQ at 303.132 kb on - strand, within yhbQ at 303.133 kb on + strand, within yhbQ at 303.526 kb on + strand, within yhbS at 303.596 kb on - strand, within yhbS
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 301,097 - yraQ BNILDI_01520 0.85 -0.2 301,129 + yraQ BNILDI_01520 0.88 +0.7 301,164 + +0.5 301,255 + -0.1 301,255 + -0.4 301,272 - +0.5 301,276 + +0.9 301,277 - -0.1 301,298 - +0.6 301,458 - yraR BNILDI_01525 0.20 +0.6 301,458 - yraR BNILDI_01525 0.20 +1.1 301,505 - yraR BNILDI_01525 0.28 +0.1 301,648 + yraR BNILDI_01525 0.50 -0.4 301,648 + yraR BNILDI_01525 0.50 -1.5 301,649 - yraR BNILDI_01525 0.50 -0.4 301,829 - yraR BNILDI_01525 0.79 +0.1 301,953 - +0.0 302,020 - +0.9 302,020 - +0.4 302,034 + +0.1 302,035 - +0.1 302,097 - -0.3 302,144 + yhbO BNILDI_01530 0.10 +0.5 302,144 + yhbO BNILDI_01530 0.10 -0.5 302,325 + yhbO BNILDI_01530 0.45 -0.2 302,357 + yhbO BNILDI_01530 0.51 +0.6 302,732 - +0.2 302,786 + +0.4 302,806 - BNILDI_01535 0.16 +0.7 302,902 + BNILDI_01535 0.51 -0.2 303,047 - -0.2 303,047 - -0.4 303,048 + +0.1 303,048 + +0.2 303,048 + -1.9 303,049 - +0.1 303,065 + -0.1 303,102 - +1.1 303,131 + yhbQ BNILDI_01540 0.16 -0.5 303,132 - yhbQ BNILDI_01540 0.16 -0.1 303,132 - yhbQ BNILDI_01540 0.16 -0.3 303,133 + yhbQ BNILDI_01540 0.17 +0.5 303,526 + yhbS BNILDI_01545 0.31 -0.4 303,596 - yhbS BNILDI_01545 0.44 -0.8
Or see this region's nucleotide sequence