Strain Fitness in Escherichia coli ECRC62 around BNILDI_01320

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdlsT and tdcG are separated by 274 nucleotidestdcG and tdcF are separated by 71 nucleotidestdcF and tdcE are separated by 13 nucleotides BNILDI_01315: dlsT - putative serine transporter, at 256,307 to 257,638 dlsT BNILDI_01320: tdcG - L-serine ammonia-lyase, at 257,913 to 259,277 tdcG BNILDI_01325: tdcF - enamine/imine deaminase, at 259,349 to 259,738 tdcF BNILDI_01330: tdcE - 2-ketobutyrate formate-lyase/pyruvate formate-lyase, at 259,752 to 262,046 tdcE Position (kb) 257 258 259 260Strain fitness (log2 ratio) -2 -1 0 1 2at 256.926 kb on - strand, within dlsTat 256.969 kb on + strand, within dlsTat 256.970 kb on - strand, within dlsTat 256.972 kb on + strand, within dlsTat 256.973 kb on - strand, within dlsTat 257.023 kb on - strand, within dlsTat 257.166 kb on + strand, within dlsTat 257.166 kb on + strand, within dlsTat 257.209 kb on + strand, within dlsTat 257.209 kb on + strand, within dlsTat 257.210 kb on - strand, within dlsTat 257.210 kb on - strand, within dlsTat 257.218 kb on - strand, within dlsTat 257.218 kb on - strand, within dlsTat 257.283 kb on + strand, within dlsTat 257.294 kb on + strand, within dlsTat 257.318 kb on + strand, within dlsTat 257.318 kb on + strand, within dlsTat 257.319 kb on - strand, within dlsTat 257.344 kb on - strand, within dlsTat 257.351 kb on - strand, within dlsTat 257.374 kb on + strand, within dlsTat 257.375 kb on - strand, within dlsTat 257.380 kb on + strand, within dlsTat 257.380 kb on + strand, within dlsTat 257.381 kb on - strand, within dlsTat 257.400 kb on + strand, within dlsTat 257.401 kb on - strand, within dlsTat 257.401 kb on - strand, within dlsTat 257.512 kb on + strandat 257.512 kb on + strandat 257.513 kb on - strandat 257.533 kb on + strandat 257.708 kb on - strandat 257.963 kb on - strandat 257.966 kb on + strandat 257.976 kb on - strandat 257.993 kb on - strandat 257.993 kb on - strandat 258.012 kb on - strandat 258.092 kb on + strand, within tdcGat 258.115 kb on - strand, within tdcGat 258.183 kb on + strand, within tdcGat 258.299 kb on + strand, within tdcGat 258.302 kb on - strand, within tdcGat 258.379 kb on + strand, within tdcGat 258.478 kb on + strand, within tdcGat 258.478 kb on + strand, within tdcGat 258.525 kb on - strand, within tdcGat 258.687 kb on + strand, within tdcGat 258.716 kb on + strand, within tdcGat 258.717 kb on - strand, within tdcGat 258.731 kb on - strand, within tdcGat 258.847 kb on + strand, within tdcGat 258.847 kb on + strand, within tdcGat 258.848 kb on - strand, within tdcGat 258.848 kb on - strand, within tdcGat 258.850 kb on + strand, within tdcGat 258.851 kb on - strand, within tdcGat 258.851 kb on - strand, within tdcGat 258.857 kb on + strand, within tdcGat 258.861 kb on + strand, within tdcGat 258.939 kb on - strand, within tdcGat 259.011 kb on + strand, within tdcGat 259.019 kb on + strand, within tdcGat 259.020 kb on - strand, within tdcGat 259.129 kb on + strand, within tdcGat 259.145 kb on + strandat 259.146 kb on - strandat 259.165 kb on - strandat 259.165 kb on - strandat 259.190 kb on + strandat 259.271 kb on + strandat 259.350 kb on + strandat 259.351 kb on - strandat 259.357 kb on + strandat 259.358 kb on - strandat 259.358 kb on - strandat 259.467 kb on + strand, within tdcFat 259.467 kb on + strand, within tdcFat 259.467 kb on + strand, within tdcFat 259.467 kb on + strand, within tdcFat 259.468 kb on - strand, within tdcFat 259.690 kb on - strand, within tdcFat 259.738 kb on + strandat 259.738 kb on + strandat 259.739 kb on - strandat 259.767 kb on - strandat 259.958 kb on - strandat 259.988 kb on + strand, within tdcEat 259.989 kb on - strand, within tdcEat 260.038 kb on + strand, within tdcEat 260.060 kb on - strand, within tdcEat 260.216 kb on + strand, within tdcE

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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256,926 - dlsT BNILDI_01315 0.46 +0.3
256,969 + dlsT BNILDI_01315 0.50 +0.0
256,970 - dlsT BNILDI_01315 0.50 -0.4
256,972 + dlsT BNILDI_01315 0.50 +0.1
256,973 - dlsT BNILDI_01315 0.50 +0.4
257,023 - dlsT BNILDI_01315 0.54 +0.1
257,166 + dlsT BNILDI_01315 0.64 +1.2
257,166 + dlsT BNILDI_01315 0.64 +0.2
257,209 + dlsT BNILDI_01315 0.68 -0.2
257,209 + dlsT BNILDI_01315 0.68 -0.6
257,210 - dlsT BNILDI_01315 0.68 +0.4
257,210 - dlsT BNILDI_01315 0.68 +0.6
257,218 - dlsT BNILDI_01315 0.68 +0.7
257,218 - dlsT BNILDI_01315 0.68 -0.6
257,283 + dlsT BNILDI_01315 0.73 -1.0
257,294 + dlsT BNILDI_01315 0.74 +0.2
257,318 + dlsT BNILDI_01315 0.76 +0.3
257,318 + dlsT BNILDI_01315 0.76 +0.2
257,319 - dlsT BNILDI_01315 0.76 -0.4
257,344 - dlsT BNILDI_01315 0.78 -1.1
257,351 - dlsT BNILDI_01315 0.78 -0.2
257,374 + dlsT BNILDI_01315 0.80 +0.6
257,375 - dlsT BNILDI_01315 0.80 +1.8
257,380 + dlsT BNILDI_01315 0.81 -0.4
257,380 + dlsT BNILDI_01315 0.81 -0.7
257,381 - dlsT BNILDI_01315 0.81 -0.1
257,400 + dlsT BNILDI_01315 0.82 +0.7
257,401 - dlsT BNILDI_01315 0.82 -2.4
257,401 - dlsT BNILDI_01315 0.82 -1.2
257,512 + +0.3
257,512 + -0.9
257,513 - +2.3
257,533 + -0.7
257,708 - +1.0
257,963 - -1.0
257,966 + +0.3
257,976 - +0.8
257,993 - -0.0
257,993 - -0.1
258,012 - +0.6
258,092 + tdcG BNILDI_01320 0.13 -0.8
258,115 - tdcG BNILDI_01320 0.15 -0.1
258,183 + tdcG BNILDI_01320 0.20 +0.7
258,299 + tdcG BNILDI_01320 0.28 -0.3
258,302 - tdcG BNILDI_01320 0.28 +0.4
258,379 + tdcG BNILDI_01320 0.34 +1.0
258,478 + tdcG BNILDI_01320 0.41 +0.4
258,478 + tdcG BNILDI_01320 0.41 -0.9
258,525 - tdcG BNILDI_01320 0.45 +1.2
258,687 + tdcG BNILDI_01320 0.57 -0.7
258,716 + tdcG BNILDI_01320 0.59 +0.2
258,717 - tdcG BNILDI_01320 0.59 -0.3
258,731 - tdcG BNILDI_01320 0.60 +0.7
258,847 + tdcG BNILDI_01320 0.68 -0.2
258,847 + tdcG BNILDI_01320 0.68 -0.6
258,848 - tdcG BNILDI_01320 0.68 -1.2
258,848 - tdcG BNILDI_01320 0.68 +0.8
258,850 + tdcG BNILDI_01320 0.69 -1.4
258,851 - tdcG BNILDI_01320 0.69 +1.1
258,851 - tdcG BNILDI_01320 0.69 +1.2
258,857 + tdcG BNILDI_01320 0.69 +1.6
258,861 + tdcG BNILDI_01320 0.69 -0.2
258,939 - tdcG BNILDI_01320 0.75 +0.6
259,011 + tdcG BNILDI_01320 0.80 -0.2
259,019 + tdcG BNILDI_01320 0.81 +0.6
259,020 - tdcG BNILDI_01320 0.81 -0.7
259,129 + tdcG BNILDI_01320 0.89 +1.5
259,145 + +0.4
259,146 - +0.1
259,165 - +0.7
259,165 - -0.2
259,190 + +0.4
259,271 + +0.8
259,350 + -0.1
259,351 - +0.3
259,357 + -0.0
259,358 - +1.5
259,358 - -1.6
259,467 + tdcF BNILDI_01325 0.30 -1.5
259,467 + tdcF BNILDI_01325 0.30 +1.2
259,467 + tdcF BNILDI_01325 0.30 +0.3
259,467 + tdcF BNILDI_01325 0.30 -0.2
259,468 - tdcF BNILDI_01325 0.31 +0.4
259,690 - tdcF BNILDI_01325 0.87 -0.2
259,738 + -1.2
259,738 + -0.2
259,739 - +0.2
259,767 - +0.7
259,958 - +0.4
259,988 + tdcE BNILDI_01330 0.10 +1.7
259,989 - tdcE BNILDI_01330 0.10 +0.4
260,038 + tdcE BNILDI_01330 0.12 -0.2
260,060 - tdcE BNILDI_01330 0.13 -1.1
260,216 + tdcE BNILDI_01330 0.20 +0.0

Or see this region's nucleotide sequence