Experiment: Sodium-DL-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnE and ygiF are separated by 22 nucleotides ygiF and ygiM are separated by 241 nucleotides ygiM and cca are separated by 63 nucleotides
BNILDI_01000: glnE - bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase, at 190,461 to 193,301
glnE
BNILDI_01005: ygiF - inorganic triphosphatase, at 193,324 to 194,625
ygiF
BNILDI_01010: ygiM - TIGR04211 family SH3 domain-containing protein, at 194,867 to 195,487
ygiM
BNILDI_01015: cca - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 195,551 to 196,789
cca
Position (kb) 193
194
195 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 192.357 kb on - strand, within glnE at 192.357 kb on - strand, within glnE at 192.442 kb on + strand, within glnE at 192.442 kb on + strand, within glnE at 192.443 kb on - strand, within glnE at 192.443 kb on - strand, within glnE at 192.443 kb on - strand, within glnE at 192.443 kb on - strand, within glnE at 192.490 kb on + strand, within glnE at 192.566 kb on - strand, within glnE at 192.568 kb on + strand, within glnE at 192.569 kb on - strand, within glnE at 192.569 kb on - strand, within glnE at 192.747 kb on - strand, within glnE at 192.783 kb on - strand, within glnE at 192.898 kb on + strand, within glnE at 192.898 kb on + strand, within glnE at 192.898 kb on + strand, within glnE at 193.010 kb on + strand, within glnE at 193.010 kb on + strand, within glnE at 193.068 kb on - strand at 193.187 kb on + strand at 193.271 kb on - strand at 193.311 kb on + strand at 193.469 kb on + strand, within ygiF at 193.470 kb on - strand, within ygiF at 193.470 kb on - strand, within ygiF at 193.470 kb on - strand, within ygiF at 193.489 kb on + strand, within ygiF at 193.501 kb on - strand, within ygiF at 193.648 kb on + strand, within ygiF at 193.716 kb on + strand, within ygiF at 193.913 kb on + strand, within ygiF at 193.913 kb on + strand, within ygiF at 193.914 kb on - strand, within ygiF at 194.160 kb on + strand, within ygiF at 194.278 kb on + strand, within ygiF at 194.332 kb on + strand, within ygiF at 194.374 kb on - strand, within ygiF at 194.376 kb on - strand, within ygiF at 194.392 kb on + strand, within ygiF at 194.436 kb on - strand, within ygiF at 194.457 kb on + strand, within ygiF at 194.512 kb on - strand at 194.592 kb on + strand at 194.598 kb on + strand at 194.632 kb on + strand at 194.633 kb on - strand at 194.633 kb on - strand at 194.633 kb on - strand at 194.636 kb on + strand at 194.644 kb on - strand at 194.644 kb on - strand at 194.652 kb on + strand at 194.652 kb on + strand at 194.653 kb on - strand at 194.653 kb on - strand at 194.675 kb on + strand at 194.697 kb on - strand at 194.700 kb on + strand at 194.701 kb on - strand at 194.703 kb on + strand at 194.704 kb on - strand at 194.828 kb on + strand at 194.829 kb on - strand at 194.832 kb on - strand at 194.892 kb on + strand at 194.965 kb on + strand, within ygiM at 195.039 kb on + strand, within ygiM at 195.042 kb on + strand, within ygiM at 195.043 kb on - strand, within ygiM at 195.065 kb on + strand, within ygiM at 195.066 kb on - strand, within ygiM at 195.066 kb on - strand, within ygiM at 195.066 kb on - strand, within ygiM at 195.122 kb on - strand, within ygiM at 195.345 kb on + strand, within ygiM at 195.345 kb on + strand, within ygiM at 195.388 kb on + strand, within ygiM at 195.388 kb on + strand, within ygiM at 195.388 kb on + strand, within ygiM at 195.388 kb on + strand, within ygiM at 195.389 kb on - strand, within ygiM at 195.392 kb on + strand, within ygiM at 195.485 kb on + strand at 195.515 kb on + strand at 195.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sodium-DL-Lactate remove 192,357 - glnE BNILDI_01000 0.67 +0.7 192,357 - glnE BNILDI_01000 0.67 +0.4 192,442 + glnE BNILDI_01000 0.70 -1.0 192,442 + glnE BNILDI_01000 0.70 -0.4 192,443 - glnE BNILDI_01000 0.70 +0.4 192,443 - glnE BNILDI_01000 0.70 -3.2 192,443 - glnE BNILDI_01000 0.70 +0.3 192,443 - glnE BNILDI_01000 0.70 -0.5 192,490 + glnE BNILDI_01000 0.71 +1.1 192,566 - glnE BNILDI_01000 0.74 -0.2 192,568 + glnE BNILDI_01000 0.74 -0.1 192,569 - glnE BNILDI_01000 0.74 -0.2 192,569 - glnE BNILDI_01000 0.74 -0.2 192,747 - glnE BNILDI_01000 0.80 -0.9 192,783 - glnE BNILDI_01000 0.82 -3.1 192,898 + glnE BNILDI_01000 0.86 -1.1 192,898 + glnE BNILDI_01000 0.86 -0.0 192,898 + glnE BNILDI_01000 0.86 +0.1 193,010 + glnE BNILDI_01000 0.90 -0.2 193,010 + glnE BNILDI_01000 0.90 +0.4 193,068 - +0.1 193,187 + +0.6 193,271 - -0.7 193,311 + +0.1 193,469 + ygiF BNILDI_01005 0.11 +0.5 193,470 - ygiF BNILDI_01005 0.11 +0.4 193,470 - ygiF BNILDI_01005 0.11 -0.2 193,470 - ygiF BNILDI_01005 0.11 -1.2 193,489 + ygiF BNILDI_01005 0.13 -0.3 193,501 - ygiF BNILDI_01005 0.14 -0.9 193,648 + ygiF BNILDI_01005 0.25 +1.2 193,716 + ygiF BNILDI_01005 0.30 +0.3 193,913 + ygiF BNILDI_01005 0.45 -0.0 193,913 + ygiF BNILDI_01005 0.45 -0.3 193,914 - ygiF BNILDI_01005 0.45 -0.1 194,160 + ygiF BNILDI_01005 0.64 -0.9 194,278 + ygiF BNILDI_01005 0.73 +0.1 194,332 + ygiF BNILDI_01005 0.77 +0.4 194,374 - ygiF BNILDI_01005 0.81 +0.0 194,376 - ygiF BNILDI_01005 0.81 +1.2 194,392 + ygiF BNILDI_01005 0.82 -0.3 194,436 - ygiF BNILDI_01005 0.85 -0.5 194,457 + ygiF BNILDI_01005 0.87 -1.6 194,512 - -0.5 194,592 + -1.2 194,598 + -1.7 194,632 + +0.6 194,633 - -0.5 194,633 - +0.2 194,633 - -2.4 194,636 + -0.4 194,644 - +0.5 194,644 - +0.0 194,652 + -2.2 194,652 + -0.1 194,653 - +0.2 194,653 - +0.7 194,675 + +0.2 194,697 - +0.9 194,700 + -1.0 194,701 - -0.8 194,703 + +0.1 194,704 - +0.5 194,828 + -1.2 194,829 - -0.2 194,832 - -2.0 194,892 + +0.2 194,965 + ygiM BNILDI_01010 0.16 -0.9 195,039 + ygiM BNILDI_01010 0.28 +1.5 195,042 + ygiM BNILDI_01010 0.28 +0.3 195,043 - ygiM BNILDI_01010 0.28 -0.7 195,065 + ygiM BNILDI_01010 0.32 +0.1 195,066 - ygiM BNILDI_01010 0.32 +0.5 195,066 - ygiM BNILDI_01010 0.32 +0.2 195,066 - ygiM BNILDI_01010 0.32 -2.4 195,122 - ygiM BNILDI_01010 0.41 +0.2 195,345 + ygiM BNILDI_01010 0.77 -0.4 195,345 + ygiM BNILDI_01010 0.77 +0.9 195,388 + ygiM BNILDI_01010 0.84 +0.2 195,388 + ygiM BNILDI_01010 0.84 +0.4 195,388 + ygiM BNILDI_01010 0.84 +0.0 195,388 + ygiM BNILDI_01010 0.84 +0.5 195,389 - ygiM BNILDI_01010 0.84 +0.1 195,392 + ygiM BNILDI_01010 0.85 +0.4 195,485 + -1.4 195,515 + +0.6 195,518 + -1.0
Or see this region's nucleotide sequence