Strain Fitness in Escherichia coli ECRC62 around BNILDI_00545

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_00535 and yghD are separated by 414 nucleotidesyghD and BNILDI_00545 are separated by 38 nucleotidesBNILDI_00545 and gspS2 are separated by 158 nucleotidesgspS2 and pppA are separated by 65 nucleotides BNILDI_00535: BNILDI_00535 - type II toxin-antitoxin system RelE/ParE family toxin, at 100,030 to 100,356 _00535 BNILDI_00540: yghD - GspM family type II secretion system protein YghD, at 100,771 to 101,307 yghD BNILDI_00545: BNILDI_00545 - Type II secretion protein GspC, at 101,346 to 101,711 _00545 BNILDI_00550: gspS2 - Pilotin AspS 2, at 101,870 to 102,280 gspS2 BNILDI_00555: pppA - prepilin peptidase PppA, at 102,346 to 103,155 pppA Position (kb) 101 102Strain fitness (log2 ratio) -3 -2 -1 0 1at 100.407 kb on - strandat 100.497 kb on + strandat 100.497 kb on + strandat 100.500 kb on - strandat 100.701 kb on - strandat 100.702 kb on + strandat 100.756 kb on + strandat 100.757 kb on - strandat 100.767 kb on + strandat 100.772 kb on + strandat 100.930 kb on - strand, within yghDat 101.043 kb on + strand, within yghDat 101.150 kb on + strand, within yghDat 101.150 kb on + strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.151 kb on - strand, within yghDat 101.188 kb on + strand, within yghDat 101.311 kb on - strandat 101.360 kb on + strandat 101.401 kb on + strand, within BNILDI_00545at 101.401 kb on + strand, within BNILDI_00545at 101.402 kb on - strand, within BNILDI_00545at 101.478 kb on - strand, within BNILDI_00545at 101.700 kb on - strandat 101.856 kb on - strandat 101.935 kb on + strand, within gspS2at 102.070 kb on + strand, within gspS2at 102.249 kb on - strandat 102.365 kb on + strandat 102.445 kb on + strand, within pppAat 102.446 kb on - strand, within pppAat 102.446 kb on - strand, within pppAat 102.507 kb on + strand, within pppAat 102.508 kb on - strand, within pppAat 102.508 kb on - strand, within pppAat 102.508 kb on - strand, within pppAat 102.511 kb on + strand, within pppAat 102.512 kb on - strand, within pppAat 102.512 kb on - strand, within pppAat 102.512 kb on - strand, within pppAat 102.526 kb on + strand, within pppAat 102.527 kb on - strand, within pppAat 102.529 kb on + strand, within pppAat 102.530 kb on - strand, within pppAat 102.530 kb on - strand, within pppA

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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100,407 - +0.4
100,497 + +0.0
100,497 + +0.4
100,500 - -0.1
100,701 - -2.8
100,702 + +1.3
100,756 + -0.6
100,757 - -2.0
100,767 + +0.9
100,772 + +1.0
100,930 - yghD BNILDI_00540 0.30 +1.2
101,043 + yghD BNILDI_00540 0.51 -2.9
101,150 + yghD BNILDI_00540 0.71 -1.0
101,150 + yghD BNILDI_00540 0.71 -0.3
101,151 - yghD BNILDI_00540 0.71 +0.0
101,151 - yghD BNILDI_00540 0.71 +0.3
101,151 - yghD BNILDI_00540 0.71 -0.2
101,151 - yghD BNILDI_00540 0.71 -0.8
101,188 + yghD BNILDI_00540 0.78 +0.5
101,311 - -0.4
101,360 + -2.0
101,401 + BNILDI_00545 0.15 +1.1
101,401 + BNILDI_00545 0.15 +0.5
101,402 - BNILDI_00545 0.15 -2.6
101,478 - BNILDI_00545 0.36 +0.8
101,700 - -1.3
101,856 - +1.3
101,935 + gspS2 BNILDI_00550 0.16 -0.2
102,070 + gspS2 BNILDI_00550 0.49 +0.0
102,249 - -0.0
102,365 + -0.6
102,445 + pppA BNILDI_00555 0.12 +0.4
102,446 - pppA BNILDI_00555 0.12 +1.3
102,446 - pppA BNILDI_00555 0.12 -0.3
102,507 + pppA BNILDI_00555 0.20 +0.3
102,508 - pppA BNILDI_00555 0.20 -0.3
102,508 - pppA BNILDI_00555 0.20 +1.1
102,508 - pppA BNILDI_00555 0.20 +0.1
102,511 + pppA BNILDI_00555 0.20 +0.3
102,512 - pppA BNILDI_00555 0.20 -0.3
102,512 - pppA BNILDI_00555 0.20 +0.6
102,512 - pppA BNILDI_00555 0.20 +1.7
102,526 + pppA BNILDI_00555 0.22 -0.9
102,527 - pppA BNILDI_00555 0.22 +0.2
102,529 + pppA BNILDI_00555 0.23 +0.3
102,530 - pppA BNILDI_00555 0.23 -0.4
102,530 - pppA BNILDI_00555 0.23 +1.2

Or see this region's nucleotide sequence