Strain Fitness in Escherichia coli ECRC62 around BNILDI_16445

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_16430 and BNILDI_16435 are separated by 263 nucleotidesBNILDI_16435 and ydiE are separated by 3 nucleotidesydiE and aroH are separated by 131 nucleotidesaroH and ppsR are separated by 156 nucleotides BNILDI_16430: BNILDI_16430 - Replication protein, at 3,288,531 to 3,291,113 _16430 BNILDI_16435: BNILDI_16435 - Uncharacterized conserved protein, at 3,291,377 to 3,291,718 _16435 BNILDI_16440: ydiE - Uncharacterized protein YdiE, at 3,291,722 to 3,291,913 ydiE BNILDI_16445: aroH - 3-deoxy-7-phosphoheptulonate synthase AroH, at 3,292,045 to 3,293,091 aroH BNILDI_16450: ppsR - posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR, at 3,293,248 to 3,294,081 ppsR Position (kb) 3292 3293 3294Strain fitness (log2 ratio) -3 -2 -1 0 1at 3291.184 kb on - strandat 3291.262 kb on - strandat 3291.430 kb on + strand, within BNILDI_16435at 3291.599 kb on - strand, within BNILDI_16435at 3291.662 kb on + strand, within BNILDI_16435at 3291.662 kb on + strand, within BNILDI_16435at 3291.724 kb on - strandat 3291.768 kb on + strand, within ydiEat 3291.812 kb on + strand, within ydiEat 3291.813 kb on - strand, within ydiEat 3291.899 kb on - strandat 3291.904 kb on + strandat 3291.907 kb on - strandat 3292.061 kb on - strandat 3292.243 kb on - strand, within aroHat 3292.288 kb on - strand, within aroHat 3292.322 kb on - strand, within aroHat 3292.373 kb on + strand, within aroHat 3292.398 kb on + strand, within aroHat 3292.399 kb on - strand, within aroHat 3292.401 kb on + strand, within aroHat 3292.402 kb on - strand, within aroHat 3292.402 kb on - strand, within aroHat 3292.402 kb on - strand, within aroHat 3292.434 kb on - strand, within aroHat 3292.434 kb on - strand, within aroHat 3292.639 kb on - strand, within aroHat 3292.696 kb on + strand, within aroHat 3292.814 kb on + strand, within aroHat 3292.815 kb on - strand, within aroHat 3292.825 kb on + strand, within aroHat 3292.826 kb on - strand, within aroHat 3293.093 kb on - strandat 3293.312 kb on + strandat 3293.705 kb on + strand, within ppsRat 3293.706 kb on - strand, within ppsRat 3293.710 kb on - strand, within ppsRat 3293.718 kb on + strand, within ppsRat 3293.809 kb on - strand, within ppsRat 3293.904 kb on - strand, within ppsRat 3294.016 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,291,184 - -1.5
3,291,262 - -0.6
3,291,430 + BNILDI_16435 0.15 +0.0
3,291,599 - BNILDI_16435 0.65 -0.3
3,291,662 + BNILDI_16435 0.83 -0.3
3,291,662 + BNILDI_16435 0.83 -0.1
3,291,724 - -0.2
3,291,768 + ydiE BNILDI_16440 0.24 +0.7
3,291,812 + ydiE BNILDI_16440 0.47 -0.7
3,291,813 - ydiE BNILDI_16440 0.47 -0.1
3,291,899 - -2.9
3,291,904 + +0.3
3,291,907 - -1.0
3,292,061 - +0.2
3,292,243 - aroH BNILDI_16445 0.19 +0.3
3,292,288 - aroH BNILDI_16445 0.23 -0.5
3,292,322 - aroH BNILDI_16445 0.26 -0.4
3,292,373 + aroH BNILDI_16445 0.31 -0.2
3,292,398 + aroH BNILDI_16445 0.34 +0.5
3,292,399 - aroH BNILDI_16445 0.34 -0.1
3,292,401 + aroH BNILDI_16445 0.34 +0.7
3,292,402 - aroH BNILDI_16445 0.34 +0.0
3,292,402 - aroH BNILDI_16445 0.34 +0.4
3,292,402 - aroH BNILDI_16445 0.34 -0.4
3,292,434 - aroH BNILDI_16445 0.37 -0.8
3,292,434 - aroH BNILDI_16445 0.37 -0.5
3,292,639 - aroH BNILDI_16445 0.57 -0.0
3,292,696 + aroH BNILDI_16445 0.62 -0.9
3,292,814 + aroH BNILDI_16445 0.73 -0.5
3,292,815 - aroH BNILDI_16445 0.74 -0.1
3,292,825 + aroH BNILDI_16445 0.74 +0.5
3,292,826 - aroH BNILDI_16445 0.75 +0.0
3,293,093 - -0.3
3,293,312 + +1.1
3,293,705 + ppsR BNILDI_16450 0.55 -1.1
3,293,706 - ppsR BNILDI_16450 0.55 -1.9
3,293,710 - ppsR BNILDI_16450 0.55 -0.2
3,293,718 + ppsR BNILDI_16450 0.56 -2.5
3,293,809 - ppsR BNILDI_16450 0.67 +0.6
3,293,904 - ppsR BNILDI_16450 0.79 +0.3
3,294,016 + +0.2

Or see this region's nucleotide sequence