Strain Fitness in Escherichia coli ECRC62 around BNILDI_15635

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyk and hexR are separated by 127 nucleotideshexR and zwf are separated by 337 nucleotides BNILDI_15630: pyk - pyruvate kinase, at 3,140,949 to 3,142,391 pyk BNILDI_15635: hexR - DNA-binding transcriptional regulator HexR, at 3,142,519 to 3,143,388 hexR BNILDI_15640: zwf - glucose-6-phosphate dehydrogenase, at 3,143,726 to 3,145,201 zwf Position (kb) 3142 3143 3144Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3141.519 kb on - strand, within pykat 3141.654 kb on + strand, within pykat 3141.776 kb on - strand, within pykat 3141.776 kb on - strand, within pykat 3141.818 kb on - strand, within pykat 3141.822 kb on + strand, within pykat 3141.908 kb on + strand, within pykat 3141.914 kb on + strand, within pykat 3141.979 kb on - strand, within pykat 3142.283 kb on + strandat 3142.284 kb on - strandat 3142.287 kb on + strandat 3142.326 kb on - strandat 3142.393 kb on + strandat 3142.394 kb on - strandat 3142.394 kb on - strandat 3142.473 kb on - strandat 3142.491 kb on + strandat 3142.493 kb on + strandat 3142.539 kb on + strandat 3142.539 kb on + strandat 3142.539 kb on + strandat 3142.539 kb on + strandat 3142.539 kb on + strandat 3142.597 kb on - strandat 3142.648 kb on + strand, within hexRat 3142.649 kb on - strand, within hexRat 3142.686 kb on + strand, within hexRat 3142.688 kb on + strand, within hexRat 3142.688 kb on + strand, within hexRat 3142.688 kb on + strand, within hexRat 3142.689 kb on - strand, within hexRat 3142.719 kb on + strand, within hexRat 3142.720 kb on - strand, within hexRat 3142.870 kb on - strand, within hexRat 3142.878 kb on + strand, within hexRat 3142.879 kb on - strand, within hexRat 3142.879 kb on - strand, within hexRat 3142.929 kb on + strand, within hexRat 3142.930 kb on - strand, within hexRat 3142.944 kb on + strand, within hexRat 3142.944 kb on + strand, within hexRat 3142.944 kb on + strand, within hexRat 3142.944 kb on + strand, within hexRat 3142.945 kb on - strand, within hexRat 3143.047 kb on - strand, within hexRat 3143.109 kb on - strand, within hexRat 3143.109 kb on - strand, within hexRat 3143.150 kb on + strand, within hexRat 3143.280 kb on + strand, within hexRat 3143.457 kb on + strandat 3143.461 kb on - strandat 3143.665 kb on + strandat 3143.680 kb on + strandat 3143.680 kb on + strandat 3143.692 kb on + strandat 3143.704 kb on + strandat 3143.708 kb on + strandat 3143.709 kb on - strandat 3143.959 kb on - strand, within zwfat 3143.992 kb on + strand, within zwfat 3144.257 kb on + strand, within zwf

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,141,519 - pyk BNILDI_15630 0.40 +0.7
3,141,654 + pyk BNILDI_15630 0.49 +0.3
3,141,776 - pyk BNILDI_15630 0.57 +0.3
3,141,776 - pyk BNILDI_15630 0.57 +0.9
3,141,818 - pyk BNILDI_15630 0.60 -0.3
3,141,822 + pyk BNILDI_15630 0.60 -0.5
3,141,908 + pyk BNILDI_15630 0.66 +0.3
3,141,914 + pyk BNILDI_15630 0.67 -0.3
3,141,979 - pyk BNILDI_15630 0.71 -0.0
3,142,283 + -0.1
3,142,284 - -0.2
3,142,287 + +0.2
3,142,326 - -0.3
3,142,393 + -0.5
3,142,394 - -0.1
3,142,394 - -0.3
3,142,473 - +0.4
3,142,491 + -0.3
3,142,493 + +0.2
3,142,539 + +0.0
3,142,539 + -0.3
3,142,539 + +0.2
3,142,539 + +0.1
3,142,539 + +0.3
3,142,597 - +1.2
3,142,648 + hexR BNILDI_15635 0.15 -1.9
3,142,649 - hexR BNILDI_15635 0.15 -0.1
3,142,686 + hexR BNILDI_15635 0.19 +0.1
3,142,688 + hexR BNILDI_15635 0.19 -0.2
3,142,688 + hexR BNILDI_15635 0.19 -0.3
3,142,688 + hexR BNILDI_15635 0.19 -0.2
3,142,689 - hexR BNILDI_15635 0.20 +0.0
3,142,719 + hexR BNILDI_15635 0.23 +1.0
3,142,720 - hexR BNILDI_15635 0.23 -0.0
3,142,870 - hexR BNILDI_15635 0.40 +0.6
3,142,878 + hexR BNILDI_15635 0.41 +0.1
3,142,879 - hexR BNILDI_15635 0.41 -1.4
3,142,879 - hexR BNILDI_15635 0.41 -0.7
3,142,929 + hexR BNILDI_15635 0.47 -0.5
3,142,930 - hexR BNILDI_15635 0.47 -0.5
3,142,944 + hexR BNILDI_15635 0.49 +0.1
3,142,944 + hexR BNILDI_15635 0.49 -0.3
3,142,944 + hexR BNILDI_15635 0.49 +0.3
3,142,944 + hexR BNILDI_15635 0.49 -0.1
3,142,945 - hexR BNILDI_15635 0.49 +0.3
3,143,047 - hexR BNILDI_15635 0.61 +0.4
3,143,109 - hexR BNILDI_15635 0.68 -0.4
3,143,109 - hexR BNILDI_15635 0.68 -0.5
3,143,150 + hexR BNILDI_15635 0.73 -0.3
3,143,280 + hexR BNILDI_15635 0.87 -0.3
3,143,457 + +0.9
3,143,461 - -1.3
3,143,665 + +3.1
3,143,680 + +0.2
3,143,680 + +0.0
3,143,692 + -0.4
3,143,704 + -3.4
3,143,708 + +0.9
3,143,709 - -0.4
3,143,959 - zwf BNILDI_15640 0.16 -0.8
3,143,992 + zwf BNILDI_15640 0.18 -0.5
3,144,257 + zwf BNILDI_15640 0.36 -1.2

Or see this region's nucleotide sequence