Strain Fitness in Escherichia coli ECRC62 around BNILDI_14850

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcbtA and yeeT are separated by 73 nucleotidesyeeT and tnp-ISL3 are separated by 352 nucleotidestnp-ISL3 and BNILDI_14855 are separated by 651 nucleotidesBNILDI_14855 and BNILDI_14860 are separated by 21 nucleotides BNILDI_14840: cbtA - type IV toxin-antitoxin system toxin CbtA, at 2,993,427 to 2,993,795 cbtA BNILDI_14845: yeeT - Uncharacterized protein YeeT, at 2,993,869 to 2,994,090 yeeT BNILDI_14850: tnp-ISL3 - ISL3 family transposase, at 2,994,443 to 2,995,822 tnp-ISL3 BNILDI_14855: BNILDI_14855 - phosphotriesterase-related protein, at 2,996,474 to 2,996,800 _14855 BNILDI_14860: BNILDI_14860 - IS3 element transposase, at 2,996,822 to 2,997,358 _14860 Position (kb) 2994 2995 2996Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2993.518 kb on + strand, within cbtAat 2993.518 kb on + strand, within cbtAat 2993.977 kb on - strand, within yeeTat 2993.978 kb on + strand, within yeeTat 2993.978 kb on + strand, within yeeTat 2994.263 kb on + strandat 2994.466 kb on + strandat 2994.466 kb on + strandat 2994.477 kb on + strandat 2994.478 kb on - strandat 2994.484 kb on - strandat 2994.515 kb on - strandat 2994.517 kb on + strandat 2994.518 kb on - strandat 2994.634 kb on - strand, within tnp-ISL3at 2994.634 kb on - strand, within tnp-ISL3at 2994.634 kb on - strand, within tnp-ISL3at 2994.654 kb on + strand, within tnp-ISL3at 2994.664 kb on - strand, within tnp-ISL3at 2994.798 kb on + strand, within tnp-ISL3at 2994.799 kb on - strand, within tnp-ISL3at 2994.801 kb on + strand, within tnp-ISL3at 2994.862 kb on + strand, within tnp-ISL3at 2994.970 kb on - strand, within tnp-ISL3at 2994.972 kb on + strand, within tnp-ISL3at 2995.062 kb on + strand, within tnp-ISL3at 2995.193 kb on + strand, within tnp-ISL3at 2995.193 kb on + strand, within tnp-ISL3at 2995.201 kb on + strand, within tnp-ISL3at 2995.201 kb on + strand, within tnp-ISL3at 2995.202 kb on - strand, within tnp-ISL3at 2995.212 kb on + strand, within tnp-ISL3at 2995.264 kb on - strand, within tnp-ISL3at 2995.264 kb on - strand, within tnp-ISL3at 2995.360 kb on + strand, within tnp-ISL3at 2995.397 kb on - strand, within tnp-ISL3at 2995.506 kb on + strand, within tnp-ISL3at 2995.528 kb on + strand, within tnp-ISL3at 2995.529 kb on - strand, within tnp-ISL3at 2995.532 kb on + strand, within tnp-ISL3at 2995.533 kb on - strand, within tnp-ISL3at 2995.642 kb on + strand, within tnp-ISL3at 2995.738 kb on + strandat 2995.738 kb on + strandat 2995.739 kb on - strandat 2995.741 kb on + strandat 2995.742 kb on - strandat 2995.836 kb on + strandat 2995.836 kb on + strandat 2995.837 kb on - strandat 2995.924 kb on - strandat 2996.006 kb on + strandat 2996.007 kb on - strandat 2996.007 kb on - strandat 2996.007 kb on - strandat 2996.032 kb on + strandat 2996.122 kb on + strandat 2996.127 kb on - strandat 2996.160 kb on - strandat 2996.359 kb on + strandat 2996.432 kb on + strandat 2996.476 kb on - strandat 2996.506 kb on + strandat 2996.773 kb on + strandat 2996.794 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,993,518 + cbtA BNILDI_14840 0.25 -2.7
2,993,518 + cbtA BNILDI_14840 0.25 +0.3
2,993,977 - yeeT BNILDI_14845 0.49 +1.0
2,993,978 + yeeT BNILDI_14845 0.49 -0.7
2,993,978 + yeeT BNILDI_14845 0.49 -0.2
2,994,263 + -0.2
2,994,466 + +0.5
2,994,466 + +1.2
2,994,477 + -0.6
2,994,478 - -0.4
2,994,484 - +1.0
2,994,515 - +0.4
2,994,517 + -0.7
2,994,518 - +1.7
2,994,634 - tnp-ISL3 BNILDI_14850 0.14 -1.0
2,994,634 - tnp-ISL3 BNILDI_14850 0.14 +0.3
2,994,634 - tnp-ISL3 BNILDI_14850 0.14 -0.9
2,994,654 + tnp-ISL3 BNILDI_14850 0.15 -1.2
2,994,664 - tnp-ISL3 BNILDI_14850 0.16 +0.7
2,994,798 + tnp-ISL3 BNILDI_14850 0.26 -0.6
2,994,799 - tnp-ISL3 BNILDI_14850 0.26 -0.3
2,994,801 + tnp-ISL3 BNILDI_14850 0.26 +0.1
2,994,862 + tnp-ISL3 BNILDI_14850 0.30 +0.1
2,994,970 - tnp-ISL3 BNILDI_14850 0.38 -1.4
2,994,972 + tnp-ISL3 BNILDI_14850 0.38 +0.4
2,995,062 + tnp-ISL3 BNILDI_14850 0.45 +1.2
2,995,193 + tnp-ISL3 BNILDI_14850 0.54 +0.1
2,995,193 + tnp-ISL3 BNILDI_14850 0.54 -0.7
2,995,201 + tnp-ISL3 BNILDI_14850 0.55 -0.2
2,995,201 + tnp-ISL3 BNILDI_14850 0.55 +0.2
2,995,202 - tnp-ISL3 BNILDI_14850 0.55 -0.5
2,995,212 + tnp-ISL3 BNILDI_14850 0.56 -1.1
2,995,264 - tnp-ISL3 BNILDI_14850 0.59 +0.1
2,995,264 - tnp-ISL3 BNILDI_14850 0.59 +0.8
2,995,360 + tnp-ISL3 BNILDI_14850 0.66 -0.2
2,995,397 - tnp-ISL3 BNILDI_14850 0.69 +0.4
2,995,506 + tnp-ISL3 BNILDI_14850 0.77 -0.2
2,995,528 + tnp-ISL3 BNILDI_14850 0.79 -2.0
2,995,529 - tnp-ISL3 BNILDI_14850 0.79 +0.1
2,995,532 + tnp-ISL3 BNILDI_14850 0.79 -0.1
2,995,533 - tnp-ISL3 BNILDI_14850 0.79 -0.6
2,995,642 + tnp-ISL3 BNILDI_14850 0.87 -1.1
2,995,738 + +0.8
2,995,738 + +3.6
2,995,739 - +0.0
2,995,741 + +0.3
2,995,742 - -0.1
2,995,836 + +0.9
2,995,836 + -1.7
2,995,837 - -0.3
2,995,924 - +0.3
2,996,006 + -0.9
2,996,007 - +1.1
2,996,007 - -1.0
2,996,007 - -0.3
2,996,032 + -1.9
2,996,122 + +0.1
2,996,127 - +0.9
2,996,160 - -0.1
2,996,359 + -1.9
2,996,432 + -1.2
2,996,476 - -0.0
2,996,506 + +0.3
2,996,773 + -0.6
2,996,794 - +0.4

Or see this region's nucleotide sequence