Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaJ and wzxC are separated by 1 nucleotides wzxC and wcaK are separated by 371 nucleotides
BNILDI_14620: wcaJ - undecaprenyl-phosphate glucose phosphotransferase, at 2,953,191 to 2,954,585
wcaJ
BNILDI_14625: wzxC - colanic acid undecaprenyl disphosphate flippase WzxC, at 2,954,587 to 2,956,065
wzxC
BNILDI_14640: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 2,956,437 to 2,957,717
wcaK
Position (kb)
2954
2955
2956
2957 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2953.614 kb on + strand, within wcaJ at 2953.616 kb on + strand, within wcaJ at 2953.815 kb on + strand, within wcaJ at 2953.816 kb on - strand, within wcaJ at 2953.914 kb on - strand, within wcaJ at 2954.009 kb on - strand, within wcaJ at 2954.009 kb on - strand, within wcaJ at 2954.043 kb on - strand, within wcaJ at 2954.043 kb on - strand, within wcaJ at 2954.075 kb on + strand, within wcaJ at 2954.383 kb on - strand, within wcaJ at 2954.499 kb on + strand at 2954.500 kb on - strand at 2954.500 kb on - strand at 2954.595 kb on - strand at 2954.719 kb on - strand at 2954.751 kb on + strand, within wzxC at 2954.752 kb on - strand, within wzxC at 2954.752 kb on - strand, within wzxC at 2954.785 kb on - strand, within wzxC at 2954.907 kb on + strand, within wzxC at 2954.931 kb on + strand, within wzxC at 2954.931 kb on + strand, within wzxC at 2955.123 kb on + strand, within wzxC at 2955.123 kb on + strand, within wzxC at 2955.124 kb on - strand, within wzxC at 2955.216 kb on - strand, within wzxC at 2955.216 kb on - strand, within wzxC at 2955.216 kb on - strand, within wzxC at 2955.256 kb on + strand, within wzxC at 2955.256 kb on + strand, within wzxC at 2955.322 kb on + strand, within wzxC at 2955.556 kb on - strand, within wzxC at 2955.556 kb on - strand, within wzxC at 2955.796 kb on - strand, within wzxC at 2955.826 kb on + strand, within wzxC at 2955.853 kb on + strand, within wzxC at 2956.053 kb on - strand at 2956.107 kb on + strand at 2956.108 kb on - strand at 2956.158 kb on + strand at 2956.246 kb on - strand at 2956.426 kb on - strand at 2956.448 kb on + strand at 2956.504 kb on + strand at 2956.505 kb on - strand at 2956.700 kb on - strand, within wcaK at 2956.700 kb on - strand, within wcaK at 2956.736 kb on + strand, within wcaK at 2956.737 kb on - strand, within wcaK at 2956.833 kb on + strand, within wcaK at 2956.871 kb on + strand, within wcaK at 2956.934 kb on + strand, within wcaK at 2956.934 kb on + strand, within wcaK at 2956.943 kb on - strand, within wcaK at 2956.950 kb on + strand, within wcaK at 2957.021 kb on + strand, within wcaK
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,953,614 + wcaJ BNILDI_14620 0.30 +0.5 2,953,616 + wcaJ BNILDI_14620 0.30 -1.6 2,953,815 + wcaJ BNILDI_14620 0.45 +0.5 2,953,816 - wcaJ BNILDI_14620 0.45 +1.6 2,953,914 - wcaJ BNILDI_14620 0.52 -0.0 2,954,009 - wcaJ BNILDI_14620 0.59 +0.3 2,954,009 - wcaJ BNILDI_14620 0.59 -2.3 2,954,043 - wcaJ BNILDI_14620 0.61 -0.2 2,954,043 - wcaJ BNILDI_14620 0.61 +0.7 2,954,075 + wcaJ BNILDI_14620 0.63 -0.7 2,954,383 - wcaJ BNILDI_14620 0.85 +0.1 2,954,499 + -0.4 2,954,500 - -0.9 2,954,500 - -0.5 2,954,595 - +1.8 2,954,719 - -0.3 2,954,751 + wzxC BNILDI_14625 0.11 +0.3 2,954,752 - wzxC BNILDI_14625 0.11 -0.9 2,954,752 - wzxC BNILDI_14625 0.11 +0.6 2,954,785 - wzxC BNILDI_14625 0.13 -0.0 2,954,907 + wzxC BNILDI_14625 0.22 +0.5 2,954,931 + wzxC BNILDI_14625 0.23 -0.3 2,954,931 + wzxC BNILDI_14625 0.23 -1.4 2,955,123 + wzxC BNILDI_14625 0.36 +0.1 2,955,123 + wzxC BNILDI_14625 0.36 +0.3 2,955,124 - wzxC BNILDI_14625 0.36 +0.1 2,955,216 - wzxC BNILDI_14625 0.43 -0.3 2,955,216 - wzxC BNILDI_14625 0.43 -0.0 2,955,216 - wzxC BNILDI_14625 0.43 -0.3 2,955,256 + wzxC BNILDI_14625 0.45 -0.2 2,955,256 + wzxC BNILDI_14625 0.45 -0.1 2,955,322 + wzxC BNILDI_14625 0.50 +0.9 2,955,556 - wzxC BNILDI_14625 0.66 +0.4 2,955,556 - wzxC BNILDI_14625 0.66 +0.3 2,955,796 - wzxC BNILDI_14625 0.82 +0.5 2,955,826 + wzxC BNILDI_14625 0.84 -0.5 2,955,853 + wzxC BNILDI_14625 0.86 -0.6 2,956,053 - +0.1 2,956,107 + -0.0 2,956,108 - -0.3 2,956,158 + -0.7 2,956,246 - +0.5 2,956,426 - -0.2 2,956,448 + -0.2 2,956,504 + -0.0 2,956,505 - -1.1 2,956,700 - wcaK BNILDI_14640 0.21 -0.0 2,956,700 - wcaK BNILDI_14640 0.21 +2.1 2,956,736 + wcaK BNILDI_14640 0.23 -1.5 2,956,737 - wcaK BNILDI_14640 0.23 -0.3 2,956,833 + wcaK BNILDI_14640 0.31 -0.8 2,956,871 + wcaK BNILDI_14640 0.34 -1.8 2,956,934 + wcaK BNILDI_14640 0.39 -0.1 2,956,934 + wcaK BNILDI_14640 0.39 +0.6 2,956,943 - wcaK BNILDI_14640 0.40 +0.7 2,956,950 + wcaK BNILDI_14640 0.40 -0.2 2,957,021 + wcaK BNILDI_14640 0.46 +0.7
Or see this region's nucleotide sequence