Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolC and araJ are separated by 8 nucleotides araJ and sapF are separated by 47 nucleotides sapF and sapD are separated by 1 nucleotides
HEPCGN_25710: tolC - multidrug transporter, at 3,880,477 to 3,881,850
tolC
HEPCGN_25715: araJ - Bcr/CflA family drug resistance efflux transporter, at 3,881,859 to 3,883,022
araJ
HEPCGN_25720: sapF - peptide ABC transporter ATP-binding protein SapF, at 3,883,070 to 3,883,876
sapF
HEPCGN_25725: sapD - peptide ABC transporter ATP-binding protein SapD, at 3,883,878 to 3,884,870
sapD
Position (kb)
3881
3882
3883
3884 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3880.993 kb on - strand, within tolC at 3881.196 kb on + strand, within tolC at 3881.255 kb on + strand, within tolC at 3881.255 kb on + strand, within tolC at 3881.502 kb on + strand, within tolC at 3881.651 kb on - strand, within tolC at 3881.859 kb on - strand at 3881.859 kb on - strand at 3881.859 kb on - strand at 3881.864 kb on + strand at 3881.934 kb on + strand at 3881.987 kb on - strand, within araJ at 3882.040 kb on + strand, within araJ at 3882.064 kb on + strand, within araJ at 3882.065 kb on - strand, within araJ at 3882.097 kb on + strand, within araJ at 3882.104 kb on + strand, within araJ at 3882.140 kb on + strand, within araJ at 3882.141 kb on - strand, within araJ at 3882.141 kb on - strand, within araJ at 3882.229 kb on - strand, within araJ at 3882.347 kb on - strand, within araJ at 3882.397 kb on + strand, within araJ at 3882.398 kb on - strand, within araJ at 3882.445 kb on + strand, within araJ at 3882.531 kb on + strand, within araJ at 3882.533 kb on + strand, within araJ at 3882.576 kb on - strand, within araJ at 3882.731 kb on - strand, within araJ at 3882.827 kb on + strand, within araJ at 3882.827 kb on + strand, within araJ at 3882.974 kb on + strand at 3882.975 kb on - strand at 3882.975 kb on - strand at 3883.020 kb on + strand at 3883.020 kb on + strand at 3883.071 kb on + strand at 3883.440 kb on + strand, within sapF at 3883.720 kb on + strand, within sapF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,880,993 - tolC HEPCGN_25710 0.38 +0.1 3,881,196 + tolC HEPCGN_25710 0.52 -0.4 3,881,255 + tolC HEPCGN_25710 0.57 -0.1 3,881,255 + tolC HEPCGN_25710 0.57 +0.7 3,881,502 + tolC HEPCGN_25710 0.75 -0.9 3,881,651 - tolC HEPCGN_25710 0.85 +0.4 3,881,859 - +0.5 3,881,859 - +0.1 3,881,859 - -0.2 3,881,864 + -0.1 3,881,934 + -0.1 3,881,987 - araJ HEPCGN_25715 0.11 +0.2 3,882,040 + araJ HEPCGN_25715 0.16 -0.2 3,882,064 + araJ HEPCGN_25715 0.18 -0.1 3,882,065 - araJ HEPCGN_25715 0.18 -2.3 3,882,097 + araJ HEPCGN_25715 0.20 +0.2 3,882,104 + araJ HEPCGN_25715 0.21 -0.1 3,882,140 + araJ HEPCGN_25715 0.24 -0.1 3,882,141 - araJ HEPCGN_25715 0.24 +0.3 3,882,141 - araJ HEPCGN_25715 0.24 -0.1 3,882,229 - araJ HEPCGN_25715 0.32 +1.8 3,882,347 - araJ HEPCGN_25715 0.42 -0.5 3,882,397 + araJ HEPCGN_25715 0.46 +0.2 3,882,398 - araJ HEPCGN_25715 0.46 -0.1 3,882,445 + araJ HEPCGN_25715 0.50 -0.6 3,882,531 + araJ HEPCGN_25715 0.58 -0.8 3,882,533 + araJ HEPCGN_25715 0.58 -0.2 3,882,576 - araJ HEPCGN_25715 0.62 -0.8 3,882,731 - araJ HEPCGN_25715 0.75 +0.3 3,882,827 + araJ HEPCGN_25715 0.83 +0.3 3,882,827 + araJ HEPCGN_25715 0.83 -0.5 3,882,974 + +0.0 3,882,975 - -1.1 3,882,975 - +0.8 3,883,020 + +1.0 3,883,020 + -0.3 3,883,071 + -1.9 3,883,440 + sapF HEPCGN_25720 0.46 -2.9 3,883,720 + sapF HEPCGN_25720 0.81 -0.9
Or see this region's nucleotide sequence