Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_25305 and HEPCGN_25310 are separated by 150 nucleotides HEPCGN_25310 and HEPCGN_25315 are separated by 4 nucleotides HEPCGN_25315 and HEPCGN_25320 overlap by 35 nucleotides HEPCGN_25320 and HEPCGN_25325 are separated by 105 nucleotides
HEPCGN_25305: HEPCGN_25305 - 9-O-acetyl-N-acetylneuraminic acid deacetylase, at 3,803,595 to 3,805,448
_25305
HEPCGN_25310: HEPCGN_25310 - holin, at 3,805,599 to 3,805,814
_25310
HEPCGN_25315: HEPCGN_25315 - DUF1327 domain-containing protein, at 3,805,819 to 3,806,163
_25315
HEPCGN_25320: HEPCGN_25320 - Ybl58, at 3,806,129 to 3,806,401
_25320
HEPCGN_25325: HEPCGN_25325 - SAR-endolysin, at 3,806,507 to 3,807,040
_25325
Position (kb)
3805
3806 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3804.616 kb on - strand, within HEPCGN_25305 at 3804.698 kb on + strand, within HEPCGN_25305 at 3804.710 kb on + strand, within HEPCGN_25305 at 3804.723 kb on - strand, within HEPCGN_25305 at 3804.723 kb on - strand, within HEPCGN_25305 at 3804.926 kb on + strand, within HEPCGN_25305 at 3805.228 kb on - strand, within HEPCGN_25305 at 3805.374 kb on - strand at 3805.392 kb on + strand at 3805.427 kb on - strand at 3805.447 kb on - strand at 3805.768 kb on - strand, within HEPCGN_25310 at 3806.167 kb on + strand, within HEPCGN_25320 at 3806.353 kb on + strand, within HEPCGN_25320 at 3806.392 kb on + strand at 3806.461 kb on + strand at 3806.461 kb on + strand at 3806.468 kb on - strand at 3806.755 kb on + strand, within HEPCGN_25325
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,804,616 - HEPCGN_25305 0.55 -0.6 3,804,698 + HEPCGN_25305 0.59 +0.3 3,804,710 + HEPCGN_25305 0.60 -0.9 3,804,723 - HEPCGN_25305 0.61 +0.4 3,804,723 - HEPCGN_25305 0.61 -1.3 3,804,926 + HEPCGN_25305 0.72 -0.6 3,805,228 - HEPCGN_25305 0.88 +0.8 3,805,374 - -0.7 3,805,392 + -0.4 3,805,427 - -0.5 3,805,447 - -0.0 3,805,768 - HEPCGN_25310 0.78 +0.1 3,806,167 + HEPCGN_25320 0.14 -1.1 3,806,353 + HEPCGN_25320 0.82 -0.8 3,806,392 + -0.5 3,806,461 + +0.4 3,806,461 + +1.2 3,806,468 - +0.5 3,806,755 + HEPCGN_25325 0.46 -5.0
Or see this region's nucleotide sequence