Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purU and ychJ are separated by 49 nucleotides ychJ and rssA are separated by 73 nucleotides
HEPCGN_25030: purU - formyltetrahydrofolate deformylase, at 3,760,588 to 3,761,430
purU
HEPCGN_25035: ychJ - YchJ family protein, at 3,761,480 to 3,761,938
ychJ
HEPCGN_25040: rssA - patatin-like phospholipase RssA, at 3,762,012 to 3,762,956
rssA
Position (kb)
3761
3762 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3760.644 kb on - strand at 3760.710 kb on - strand, within purU at 3760.710 kb on - strand, within purU at 3760.775 kb on + strand, within purU at 3760.775 kb on + strand, within purU at 3760.870 kb on - strand, within purU at 3760.925 kb on + strand, within purU at 3760.956 kb on - strand, within purU at 3761.003 kb on - strand, within purU at 3761.059 kb on + strand, within purU at 3761.059 kb on + strand, within purU at 3761.059 kb on + strand, within purU at 3761.060 kb on - strand, within purU at 3761.064 kb on + strand, within purU at 3761.065 kb on - strand, within purU at 3761.111 kb on + strand, within purU at 3761.112 kb on - strand, within purU at 3761.112 kb on - strand, within purU at 3761.154 kb on + strand, within purU at 3761.203 kb on + strand, within purU at 3761.361 kb on + strand at 3761.361 kb on + strand at 3761.365 kb on - strand at 3761.365 kb on - strand at 3761.398 kb on - strand at 3761.413 kb on - strand at 3761.557 kb on + strand, within ychJ at 3761.567 kb on - strand, within ychJ at 3761.567 kb on - strand, within ychJ at 3761.568 kb on + strand, within ychJ at 3761.569 kb on - strand, within ychJ at 3761.569 kb on - strand, within ychJ at 3761.571 kb on + strand, within ychJ at 3761.572 kb on - strand, within ychJ at 3761.572 kb on - strand, within ychJ at 3761.699 kb on + strand, within ychJ at 3761.723 kb on - strand, within ychJ at 3761.880 kb on + strand, within ychJ at 3761.881 kb on - strand, within ychJ at 3761.894 kb on + strand at 3761.894 kb on + strand at 3761.895 kb on - strand at 3761.895 kb on - strand at 3761.895 kb on - strand at 3761.902 kb on - strand at 3761.942 kb on + strand at 3761.984 kb on - strand at 3761.984 kb on - strand at 3761.984 kb on - strand at 3761.989 kb on + strand at 3761.989 kb on + strand at 3762.006 kb on - strand at 3762.017 kb on + strand at 3762.198 kb on + strand, within rssA at 3762.198 kb on + strand, within rssA at 3762.337 kb on + strand, within rssA at 3762.401 kb on + strand, within rssA at 3762.423 kb on - strand, within rssA at 3762.423 kb on - strand, within rssA at 3762.523 kb on + strand, within rssA at 3762.527 kb on + strand, within rssA at 3762.570 kb on - strand, within rssA at 3762.752 kb on + strand, within rssA at 3762.752 kb on + strand, within rssA at 3762.851 kb on - strand, within rssA at 3762.851 kb on - strand, within rssA at 3762.854 kb on + strand, within rssA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,760,644 - -0.4 3,760,710 - purU HEPCGN_25030 0.14 +0.8 3,760,710 - purU HEPCGN_25030 0.14 -0.5 3,760,775 + purU HEPCGN_25030 0.22 +0.0 3,760,775 + purU HEPCGN_25030 0.22 +1.2 3,760,870 - purU HEPCGN_25030 0.33 +0.4 3,760,925 + purU HEPCGN_25030 0.40 +0.2 3,760,956 - purU HEPCGN_25030 0.44 -0.7 3,761,003 - purU HEPCGN_25030 0.49 -0.3 3,761,059 + purU HEPCGN_25030 0.56 -0.1 3,761,059 + purU HEPCGN_25030 0.56 -1.2 3,761,059 + purU HEPCGN_25030 0.56 -0.1 3,761,060 - purU HEPCGN_25030 0.56 +0.8 3,761,064 + purU HEPCGN_25030 0.56 +1.1 3,761,065 - purU HEPCGN_25030 0.57 -0.0 3,761,111 + purU HEPCGN_25030 0.62 -0.3 3,761,112 - purU HEPCGN_25030 0.62 +0.0 3,761,112 - purU HEPCGN_25030 0.62 +0.6 3,761,154 + purU HEPCGN_25030 0.67 +0.1 3,761,203 + purU HEPCGN_25030 0.73 -1.3 3,761,361 + -0.3 3,761,361 + +0.0 3,761,365 - +0.1 3,761,365 - -0.8 3,761,398 - -0.3 3,761,413 - +0.1 3,761,557 + ychJ HEPCGN_25035 0.17 -0.1 3,761,567 - ychJ HEPCGN_25035 0.19 +1.5 3,761,567 - ychJ HEPCGN_25035 0.19 -3.0 3,761,568 + ychJ HEPCGN_25035 0.19 -0.0 3,761,569 - ychJ HEPCGN_25035 0.19 +0.8 3,761,569 - ychJ HEPCGN_25035 0.19 +0.5 3,761,571 + ychJ HEPCGN_25035 0.20 +0.5 3,761,572 - ychJ HEPCGN_25035 0.20 +0.8 3,761,572 - ychJ HEPCGN_25035 0.20 +0.5 3,761,699 + ychJ HEPCGN_25035 0.48 +0.4 3,761,723 - ychJ HEPCGN_25035 0.53 -0.5 3,761,880 + ychJ HEPCGN_25035 0.87 +0.4 3,761,881 - ychJ HEPCGN_25035 0.87 -0.0 3,761,894 + +0.2 3,761,894 + -0.7 3,761,895 - +0.1 3,761,895 - +0.5 3,761,895 - +0.7 3,761,902 - -0.4 3,761,942 + +0.3 3,761,984 - -0.4 3,761,984 - -1.9 3,761,984 - +0.4 3,761,989 + +0.3 3,761,989 + +0.3 3,762,006 - +0.9 3,762,017 + +0.3 3,762,198 + rssA HEPCGN_25040 0.20 +0.3 3,762,198 + rssA HEPCGN_25040 0.20 +0.1 3,762,337 + rssA HEPCGN_25040 0.34 +0.1 3,762,401 + rssA HEPCGN_25040 0.41 +0.5 3,762,423 - rssA HEPCGN_25040 0.43 -1.9 3,762,423 - rssA HEPCGN_25040 0.43 -2.6 3,762,523 + rssA HEPCGN_25040 0.54 -0.0 3,762,527 + rssA HEPCGN_25040 0.54 +0.2 3,762,570 - rssA HEPCGN_25040 0.59 -0.0 3,762,752 + rssA HEPCGN_25040 0.78 +0.5 3,762,752 + rssA HEPCGN_25040 0.78 -0.5 3,762,851 - rssA HEPCGN_25040 0.89 -0.9 3,762,851 - rssA HEPCGN_25040 0.89 +0.2 3,762,854 + rssA HEPCGN_25040 0.89 +0.8
Or see this region's nucleotide sequence