Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cgsD and csgB are separated by 754 nucleotides csgB and csgA are separated by 40 nucleotides csgA and csgC are separated by 57 nucleotides
HEPCGN_23645: cgsD - transcriptional regulator CsgD, at 3,524,279 to 3,524,929
cgsD
HEPCGN_23655: csgB - curlin minor subunit CsgB, at 3,525,684 to 3,526,139
csgB
HEPCGN_23660: csgA - curlin major subunit CsgA, at 3,526,180 to 3,526,635
csgA
HEPCGN_23665: csgC - curli assembly protein CsgC, at 3,526,693 to 3,527,025
csgC
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3524.711 kb on - strand, within cgsD at 3524.728 kb on + strand, within cgsD at 3524.783 kb on - strand, within cgsD at 3524.783 kb on - strand, within cgsD at 3524.803 kb on + strand, within cgsD at 3524.897 kb on - strand at 3524.908 kb on + strand at 3524.912 kb on - strand at 3525.055 kb on - strand at 3525.168 kb on + strand at 3525.385 kb on + strand at 3525.483 kb on - strand at 3525.774 kb on + strand, within csgB at 3525.813 kb on - strand, within csgB at 3525.826 kb on + strand, within csgB at 3525.849 kb on - strand, within csgB at 3525.863 kb on - strand, within csgB at 3525.909 kb on - strand, within csgB at 3525.957 kb on + strand, within csgB at 3526.068 kb on + strand, within csgB at 3526.178 kb on - strand at 3526.197 kb on - strand at 3526.291 kb on - strand, within csgA at 3526.291 kb on - strand, within csgA at 3526.515 kb on + strand, within csgA at 3526.516 kb on - strand, within csgA at 3526.525 kb on - strand, within csgA at 3526.630 kb on + strand at 3526.637 kb on - strand at 3526.693 kb on - strand at 3526.743 kb on + strand, within csgC at 3526.765 kb on + strand, within csgC at 3526.765 kb on + strand, within csgC at 3526.765 kb on + strand, within csgC at 3526.766 kb on - strand, within csgC at 3526.766 kb on - strand, within csgC at 3526.891 kb on - strand, within csgC at 3526.922 kb on + strand, within csgC at 3527.123 kb on + strand at 3527.123 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,524,711 - cgsD HEPCGN_23645 0.66 +0.6 3,524,728 + cgsD HEPCGN_23645 0.69 +0.7 3,524,783 - cgsD HEPCGN_23645 0.77 -0.6 3,524,783 - cgsD HEPCGN_23645 0.77 -2.8 3,524,803 + cgsD HEPCGN_23645 0.80 +0.2 3,524,897 - +0.6 3,524,908 + +0.0 3,524,912 - +0.2 3,525,055 - -0.2 3,525,168 + +0.3 3,525,385 + +0.5 3,525,483 - +1.7 3,525,774 + csgB HEPCGN_23655 0.20 +0.2 3,525,813 - csgB HEPCGN_23655 0.28 -0.2 3,525,826 + csgB HEPCGN_23655 0.31 -0.6 3,525,849 - csgB HEPCGN_23655 0.36 +0.2 3,525,863 - csgB HEPCGN_23655 0.39 +0.1 3,525,909 - csgB HEPCGN_23655 0.49 -1.0 3,525,957 + csgB HEPCGN_23655 0.60 +0.2 3,526,068 + csgB HEPCGN_23655 0.84 -0.3 3,526,178 - +0.2 3,526,197 - +0.1 3,526,291 - csgA HEPCGN_23660 0.24 +0.5 3,526,291 - csgA HEPCGN_23660 0.24 +0.4 3,526,515 + csgA HEPCGN_23660 0.73 -0.3 3,526,516 - csgA HEPCGN_23660 0.74 -2.8 3,526,525 - csgA HEPCGN_23660 0.76 -1.0 3,526,630 + +0.4 3,526,637 - +1.3 3,526,693 - -0.5 3,526,743 + csgC HEPCGN_23665 0.15 -0.1 3,526,765 + csgC HEPCGN_23665 0.22 -0.4 3,526,765 + csgC HEPCGN_23665 0.22 +0.0 3,526,765 + csgC HEPCGN_23665 0.22 +0.2 3,526,766 - csgC HEPCGN_23665 0.22 -0.1 3,526,766 - csgC HEPCGN_23665 0.22 +0.0 3,526,891 - csgC HEPCGN_23665 0.59 -0.1 3,526,922 + csgC HEPCGN_23665 0.69 +0.1 3,527,123 + +0.1 3,527,123 + -0.1
Or see this region's nucleotide sequence